Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Adicionar filtros








Intervalo de ano
1.
Chinese Journal of Experimental and Clinical Virology ; (6): 182-184, 2009.
Artigo em Chinês | WPRIM | ID: wpr-325597

RESUMO

<p><b>OBJECTIVE</b>To construct chimerical DNA vaccine plasmid of human papillomavirus type 11 (HPV11) L1-E7, and to evaluate its immunogenicity.</p><p><b>METHODS</b>Molecular cloning techniques were used to construct recombinant plasmid pcDNA3 L1-E7 as a DNA vaccine. BALB/c mice were vaccinated with DNA recombinants through muscle injection.IL-2 and gamma-INF secreted by immunized spleens lymphocyte and HPV 11 L1 or E7 specific antibodies were assayed by ELISA method. Spleens lymphocyte proliferation was measured by MTT assay.</p><p><b>RESULTS</b>The chimerical DNA plasmid of pcDNA3 L1-E7 was constructed correctly. Specific anti-HPV11 E7 and L1 antibodies, specific lymphocyte proliferation and secretions of IL-2 and gamma-INF were detected in vaccinated mice.</p><p><b>CONCLUSION</b>Specific immune response, including cellular immunity and humoral immunity, could been detected in mice vaccinated with chimerical DNA vaccine of pcDNA3 L1-E7.</p>


Assuntos
Animais , Feminino , Humanos , Camundongos , Anticorpos Antivirais , Sangue , Alergia e Imunologia , Sequência de Bases , Engenharia Genética , Papillomavirus Humano 11 , Genética , Alergia e Imunologia , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Infecções por Papillomavirus , Sangue , Alergia e Imunologia , Virologia , Vacinas contra Papillomavirus , Genética , Alergia e Imunologia , Distribuição Aleatória , Vacinas de DNA , Genética , Alergia e Imunologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA