RESUMO
<p><b>OBJECTIVE</b>To investigate the effects of B7H4 on human bone marrow mesenchymal stem cells (HBMSC) mediating immune suppression.</p><p><b>METHODS</b>The expression of the negative immunoregulatory factor B7H4 on HBMSC were analyzed by RT-PCR and flow cytometry (FCM), respectively. The blocking experiment was used to detect the effects of B7H4 on HBMSC mediating suppression on PHA induced T cell activation, proliferation and cell cycle. HBMSC inhibiting T cell proliferation was examined by transwell cell culture system.</p><p><b>RESULTS</b>B7H4 was highly expressed on HBMSC. Blocking the B7H4 expression by B7H4mAb significantly attenuated the inhibitory effects of HBMSC on T cell proliferation. Compared with that of the unblocking group, T cell stimulator index (SI) of the B7H4 blocked group was significantly increased (53 +/- 5 vs 15 +/- 8, P < 0.01) and the inhibitory effects of HBMSC on T cell cycle were weakened significantly through down-regulating the cell number in G(0)/G(1) phase \[(85.6 +/- 9.9)% vs (95.8 +/- 9.9)%\] and up-regulating those in S phase\[(5.8 +/- 3.2)% vs (2.3 +/- 2.2)%, P < 0.05\]. The suppressive effects of HBMSC on T cell proliferation were significantly weakened after separating HBMSC from T cells by transwell cell culture system. Compared with the cell to cell contact group, T cell SI was significantly increased (27 +/- 17 vs 15 +/- 3, P < 0.01).</p><p><b>CONCLUSION</b>HBMSC highly express B7H4, which plays an important role in the suppressive effects of HBMSC on T cell proliferation.</p>
Assuntos
Humanos , Antígeno B7-1 , Metabolismo , Fisiologia , Células da Medula Óssea , Alergia e Imunologia , Metabolismo , Ciclo Celular , Alergia e Imunologia , Proliferação de Células , Células Cultivadas , Ativação Linfocitária , Alergia e Imunologia , Células-Tronco Mesenquimais , Alergia e Imunologia , Metabolismo , Fito-Hemaglutininas , Farmacologia , Linfócitos T , Biologia Celular , Alergia e Imunologia , Inibidor 1 da Ativação de Células T com Domínio V-SetRESUMO
The immunoregulatory effects of mescenchymal stem cell (MSC) and its application have become a hot research topic in recent years. This article reviews the up-to-dated research advances in the features and mechanisms of immune regulation of MSC and its application.
Assuntos
Animais , Humanos , Subpopulações de Linfócitos , Alergia e Imunologia , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais , Fisiologia , Linfócitos T Reguladores , Alergia e ImunologiaRESUMO
<p><b>OBJECTIVE</b>To study the effects of rhizoma sparganii and radices zedoariae on hepatic fibrosis.</p><p><b>METHOD</b>The rat immunohepatic fibrosis model was made by intraperitoneal injection of porcine serum and treated with rhizoma sparganii and radices zedoariae. The ALT, GGT, TP, ALb, A/G, IVC, LN, HA and the pathological change of the liver were observed.</p><p><b>RESULT</b>Rhizoma sparganii and radices zedoariae could increase TP, ALb, A/G, decrease ALT, GGT, IVC, LN, HA and improve the pathological change.</p><p><b>CONCLUSION</b>Rhizoma sparganii and radices zedoariae can protect hepatic cells, alleviate degeneration and necrosis, recover structure and function, and reduce the proliferation of fibrous tissue.</p>