Identification of Fel Serpentis and its adulterants with specific PCR method / 中国中药杂志

The study aims at developing a convenient and specific method for the identification of Fel Serpentis DNA. The methods of Fel Serpentis genomic DNA purification were tested and optimized, four pairs of specific primers for the amplification of COⅠ, Cyt b and 16S were designed. Then the best pair of primers were selected according to the specificity and efficiency. The DNA fragment about 400 bp was amplified from 20 kinds of Fel Serpentis, whereas no DNA fragment was amplified from other animal samples under the same condition. This method is specific,accurate and reproducible, which provides a useful tool for the quality control of Fel Serpentis.

Market quality surveillance and analysis of chinese medicinal materials and decoction pieces in 2017 / 中国中药杂志

With the rapid development of traditional Chinese medicine (TCM) in China as well as the implementation of the four most strict requirements, the quality of Chinese medicinal materials and decoction pieces had beem improved in recent years, however new problems and challenges were occurred. All the data of Chinese medicinal materials and decoction piece in special inspection,supervision test and evaluation inspection of drug administration department to were summarized and analyzed evaluate and analyze of the quality of Chinese medicinal materials and decoction pieces in 2017. On this basis, the relevant quality control strategies and suggestions were put forward for the relevant departments of China Food and Drug Administration to formulate and implement regulatory measures, furthermore to improve drug standards, and ensure the safety of medication.

The analysis of multivariate image and chemometrics in TLC fingerprinting of artificial cow-bezoar / 中国中药杂志

A method of thin-layer fingerprinting chromatogram of artificial cow-bezoar was established with the developing solvent consisting of cyclohexane, ethyl acetate, acetic acid and methanol (2∶7∶1∶2), and 10% sulfuric acid ethanol solution sprayed as colour-developing agent. After heated at 105 ℃, TLC was recorded as an image in ultraviolet light at 366 nm which was converted into grayscale. By the gray value extracted from the grayscale, the multivariate data obtained from TLC of samples could be analyzed by chemometric method. The results indicated that samples from different manufacturers could be distinguished by this method and some specific bands were found out. All in one, this simple and practical method was suitable for the evaluation of quality difference.

Identification study of glue medicines / 中国药学杂志

OBJECTIVE: To find the marker peptides of five kinds of glue medicines including deerhorn glue, tortoise shell glue, donkey-hide gelatin, bovine-hide gelatin, and pig-hide gelatin. METHODS: The characteristic peptides of different gelatins were identified by tripsin digestion and HPLC-MS/MS. RESULTS: The marker peptides of five glue medicines were found, ie, donkey-hide gelatin m/z 539.8, deerhorn glue m/z 765.4, tortoise shell glue m/z 631.3, bovinehide gelatin m/z 641.3, pig-hide gelatin m/z 774.5, and the methods which can be used to identify the glue medicines were established according to the marker peptides. CONCLUSION: The marker peptides that we found can be used for the identification of glue medicines.

Detection of artificial musk in Xihuangwan by gas chromatography-mass spectrometery / 中国医学科学院学报

<p><b>OBJECTIVE</b>To control the quality of Xihuangwan by improving the qualitative detection of artificial musk inside this pill.</p><p><b>METHOD</b>The qualitative detection of artificial musk was carried out by gas chromatography-mass spectrometery(GC-MS).</p><p><b>RESULT</b>The established GC-MS successfully detected artificial musk in Xihuangwan.</p><p><b>CONCLUSION</b>GC-MS is reliable,accurate,and practical in the identification of artificial must inside Xihuangwan.</p>

Determination of the contents of five heavy metals in artificial musk / 中国医学科学院学报

<p><b>OBJECTIVE</b>To evaluate the safety of artificial musk by determining its content of five hazard heavy metals.</p><p><b>METHOD</b>The contents of Pb, Cd,As,Hg,and Cu in artificial musk were detected using inductively coupled plasma-mass spectrometry.</p><p><b>RESULT</b>The contents of Pb,Cd,As,Hg,and Cu was less than 0.35,0.05,0.30,0.20,and 4.50 mg/kg in artificial musk.</p><p><b>CONCLUSION</b>Artificial musk has low contents of heavy metals and therefore meets the safety and quality requirments.</p>

Chemical constituents of surface layer of Poria cocos and their pharmacological properties (I) / 中国中药杂志

The surface layer of the sclerotia of Poria cocos, named Fu-Ling-Pi, is used as a diuretic in traditional Chinese medicine to treat edema and urinary dysfunction. Recent studies have showed that the triterpenes (lanostane and 3,4-secolanostane skeletons) and polysaccharides (beta-pachyman) are the main components of Fu-Ling-Pi and they exhibited various biological activities, such as anti-tumor, antibacterial and antioxidant, etc. This review was focused on the chemistry, pharmacology, and clinical uses of this drug and it may provide scientific foundation for further development and utilization of Fulingpi.

Euphorbia kansui roots induced-diarrhea in mice correlates with inflammatory response / 中国天然药物

AIM@#Euphorbia kansui (E. KS) is a traditional medicine used in China for thousands of years with the effect of propulsion in the gastrointestines. However, there is no reported study of E. KS on gastrointestinal motility until now. The aim of this work is to study the effect of E. KS on the propulsion of gastrointestines, and to elucidate the possible mechanism of action.@*METHODS@#E.KS was prepared as a 30% ethanol extract and used for the experiment of small and large intestines of mice by oral administration with three different dosages (1.2, 0.6 and 0.3 g·kg(-1)). The feces were observed in vivo. The morphology was carried out to detect if there are any changes in the intestines after the extract of E. KS administration. The assays of mRNA and protein expression were employed to observe IL-1β, TNFα and caspase 3.@*RESULTS@#It was shown that the extract of E.KS promoted diarrhea in mouse feces after administration, inhibited the contraction of smooth muscle of mouse small intestine and caused the inflammatory exudation on the mucosa of the intestines, enhanced the expression of both mRNA and the protein levels of IL-1β and TNFα in the small or large intestines.@*CONCLUSION@#The results showed that the extract of E. KS acted on the intestinal smooth muscle with propulsion of feces involving the irritation of the intestines with acute inflammatory reactions.