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@#Abstract: Objective To analyze the whole genome characteristic of influenza A(H1N1) pdm09 virus outbreak in a school in Jining, so as to serve as a reference for prevention and control of influenza A (H1N1) pdm09．Methods Real time fluorescence quantitative PCR were used to detected the 12 nasopharyngeal swab specimens from patients. The positive specimens were cultured and sequenced the whole genome. DNASTAR was used to analyze the homology. MEGA was used to construct the phylogenetic tree. NetNGly, GPS-SUMO, NetPhos were used to predict the glycosylation sites, the small ubiquitin-like modifier sites and the phosphorylation sites respectively. Results Among of 12 specimens, 4 specimens were showed positive for influenza A (H1N1) pdm09 virus nucleic acid, and 4 virus strains were isolated. Compared to the vaccine strain A/Brisbane/02/2018, the homology of nucleic acids of 8 gene segments was 98.5%-99.8%. The homology of amino acids of 10 proteins was 98.2%-100%. In the evolutionary tree analysis, four strains were located in two evolutionary clusters, three strains in the same evolutionary cluster and one strain in a different evolutionary cluster. A total of 50 amino acid site substitutions occurred in the 10 proteins encoded by the four strains, including two amino acid sites in the hemagglutinin antigen epitope, and there was no mutation in the neuraminidase (NA) protease active site, polymerase acidic protein (PA), polymerase basic protein 1 (PB1) and polymerase basic protein 2 (PB2) inhibitor resistance sites, and neuraminidase inhibitor resistance sites. In addition, the glycosylation sites and SUMO sites were not mutated, while multiple phosphorylation sites were substituted. Conclusions There are 2 different sources of influenza A (H1N1) virus causing the school influenza outbreak. School influenza outbreak surveillance and school influenza prevention and control should be strengthened should be enhanced.
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<p><b>OBJECTIVE</b>To study the value and the clinical application of the Medical Image three-dimensional Visualization System of Abdomen (MI-3DVS) in diagnosis and evaluating resectability of pancreatic tumor.</p><p><b>METHODS</b>Twelve patients with pancreatic tumor were tested with 64-slice helical CT (64-MSCT) angiography, and the CT data was reconstructed with MI-3DVS from November 2008 to August 2009. The 3D findings were adopted in diagnosis and evaluating resectability, and the results were compared with surgical operation and the pathological finding. There were 7 male and 5 female, aged from 14 to 83 years. Within the 12 cases, there were 4 cases with pancreatic carcinoma, 5 cases with pancreatic solid pseudopapillary tumor, 2 cases with pancreatic serous cystadenoma, 1 case with pancreatic cyst (ductal epithelial papillary hyperplasia).</p><p><b>RESULTS</b>Nine tumors which had been regarded as removable pre-operatively with MI-3DVS were removed successfully. Three patients who were considered unresectable by other hospitals with CT were operated successfully with MI-3DVS. The other 3 patients' tumors were actually not able to be removed as pre-operative evaluation.</p><p><b>CONCLUSION</b>MI-3DVS plays an important role in diagnosis and assessment of resectability of pancreatic tumor.</p>