HLA-E siRNA silence expression of HLA-E gene in hepatocarcinomal cells / 中国药理学通报

Aim According to various target sites of HLA-E mRNA,to design and synthesize 3 pieces of HLA-E siRNA chain,to compare quantitatively their efficiency of silencing gene in BEL-7402 with HLA-E(+)in order to select the dominant siRNA.Methods The hepatocarcinomal BEL-7402 cells,induced by 5×10~5 IU·L~(-1) IFN-γ,expressed HLA-E(+) and was pured by flow cytometry selecting as target cells for research.3 pieces of specific siRNA(A,B,C group)were designed and chemically synthesized,then the concentration of which(0.1 mmol·L~(-1))was respectively transfected through Lipofectamin 2000 into target cells.After 48 h,the gene silent effect on HLA-E gene in A,B and C groups was quantitatively observed by cytoimmunofluorence,flow cytometery,Western blot and real-time PCR,as well as on NK cytotoxicity to target cells tested by NK killing rate.Results Compared with those of control or non-specific siRNA group,HLA-E antigen,protein product,HLA-E mRNA and HLA-E molecule on cell surface were statistically down-regulated in A,B,and C group(P<0.01),whose were silenced more (above 90%) in B or C group than in A group (P<0.01).The NK killing rate in A,B and C groups was dominantly improved(P<0.01),which in B or C group was higher than in A group (P<0.01).Conclusion The targeted siRNA can specifically and high-efficiently silence HLA-E expression in hepatocarcinomal cells,and may keep them from immunoescape through non-classic HLAⅠ pathway to imply new strategy for hepatocarcinomal gene-immunotherapy.

Prevention of acute renal failure in obstructive jaundice patients in perioperative period / 中国普通外科杂志

ObjectiveTo investigate the protection of renal function and the prevention of acute renal failue(ARF) in perioperative period in obstructive jaundice(OJ) patients. Methods In experimantal group, short time parenteral nutrition(PN) was given preoperatirely.Small dosage of dopamine was intravenously dripped.and rapid infusion of 20% mannitol periodically was done.In control group routine treatment was done. Postoperative blood BUN and Cr dynamic changes were examined and occurrence rate of ARF were recorded. Results No ARF occurred in the experimental group;but ARF occurred in 5 cases(29.4%)and 2 cases(17.6%) died (P