RESUMO
Objective: To study Zuojin Pills with different extraction methods and select the best one. Methods: To establish the fingerprints and determination the content of five alkaloids in Zuojin Pills by ultra performance liquid chromatography (UPLC), compare the water extraction, 70% ethanol extraction, HCl-methanol (1:100) extraction, and semi-bionic extraction according to the similarity of fingerprints and content of five alkaloids. Results: The appearance time of all chromatographic peaks of Zuojin Pills was within 8 min and 9 common chromatographic peaks were obtained. The linear is stable and peak form is better. The linear relationship and resolution of the five alkaloids are nice. The similarity of the fingerprints exceed 0.98; The contents of the five alkaloids are much more different, the content of the five alkaloids in the HCl-methanol sample is the most, however, the least is the water extraction sample. Conclusion: This study shows that the UPLC method is convenient with quick analysis rate. On account of indicated component quantitative analysis, it could be used to synthetically evaluate the extraction method. HCl-methanol (1:100) extraction method is the best one.
RESUMO
<p><b>OBJECTIVE</b>To develop a quantitative method for determination of the total organic acids and salicyclic acid in the extract of Radix Isatidis.</p><p><b>METHOD</b>The total organic acids were determined by acid-base titration and the salicylic acid was determined by HPLC.</p><p><b>RESULT</b>It was shown that contents of total organic acids and salicylic acid in the extract of Radix Isatidis were 13.0% and 0.22%, respectively.</p><p><b>CONCLUSION</b>The method can control the quality of this extract effectively and accurately.</p>