Associated study on interleukin 10 gene promoter polymorphisms related to hepatitis B virus infection in Chinese Han population / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To investigate the association that the polymorphisms of interleukin 10 gene (IL10) promoter region are related to the susceptibility and clinical phenotypes of hepatitis B virus (HBV) in Chinese Han population.</p><p><b>METHODS</b>With polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) method, the single nucleotide polymorphisms (SNP) of the promoter region of IL10 gene at position -1082G/A, -819T/C, -592A/C were detected in 231 patients with chronic hepatitis B, 165 individuals spontaneously recovered from HBV infection and 135 normal controls.</p><p><b>RESULTS</b>No significant difference was found in frequencies of genotypes and alleles of IL10 gene promoter region at position -1082G/A, -819T/C, -592A/C among normal controls, individuals spontaneously recovering from HBV infection and patients with chronic hepatitis B (P>0.05), also between patients with HBV infection with HBV-DNA<1x10(3)copies/mL and those with HBV-DNA> or =1 x 10(3)copies/mL (P>0.05). However, frequencies of TT genotype at position -819T/C and AA genotype at position -592A/C in chronic hepatitis B were significantly higher than that in asymptomatic HBV carriers (P<0.05).</p><p><b>CONCLUSION</b>It is possible that genetic polymorphisms of IL10 promoter region are not associated with both susceptibility of HBV infection and HBV-DNA replication after infected HBV in Chinese Han population. However, the polymorphisms of the promoter region IL10 at position -819T/C and -592A/C are related to inflammatory reaction to liver of the patients with HBV infection.</p>

Relationship of interleukin-18 gene promoter polymorphisms with chronic hepatitis B in Chinese Han population / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To investigate the polymorphisms of interleukin-18(IL-18) gene promoters, and to disclose whether such polymorphisms are associated with susceptibility to chronic hepatitis B in Chinese Han population.</p><p><b>METHODS</b>Using polymerase chain reaction with sequence specific primers method, the authors detected the single nucleotide polymorphisms of the promoter region of IL-18 gene at position -607C/A and -137G/C in 231 patients with chronic hepatitis B and 300 normal controls.</p><p><b>RESULTS</b>The frequency of CC genotype in IL-18 gene promoter region at position -607 was 0.22(66/300) in normal controls and 0.27(62/231) in patients. The frequency of CA genotype was 0.53(160/300) in normal controls and 0.50(116/231) in patients. The frequency of AA genotype was 0.25(74/300) in normal controls and 0.23(53/231) in patients. The frequencies of -137GG, GC and CC genotype were 0.67, 0.30 and 0.03 in normal controls respectively; whereas in chronic hepatitis B patients the frequencies were 0.79, 0.19 and 0.02. The genotype frequency of -137GG in chronic hepatitis B groups was significantly higher than that in normal controls(chi2: 8.55, P=0.003), but the frequencies of -607C/-137C and -607A/-137C haplotypes in chronic hepatitis B groups were significantly lower than those in normal controls. The association between genotype of IL-18 promoter region polymorphisms and hepatitis B virus(HBV) copies showed that the frequency of -607AA genotype in high HBV-DNA copies groups was lower than that in low HBV-DNA copies groups(chi2: 6.03, P=0.014).</p><p><b>CONCLUSION</b>The polymorphisms of the promoter region of IL-18 gene at position -607C/A and -137G/C are correlated with chronic hepatitis B in Chinese Han population. The people with -137C allele in the promoter region of IL-18 gene may be protected against HBV infection, and the IL-18 -607AA genotype may be linked to HBV-DNA copy.</p>