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1.
Journal of Forensic Medicine ; (6): 15-17, 2004.
Artigo em Chinês | WPRIM | ID: wpr-983009

RESUMO

OBJECTIVE@#The sensitivity of heart-type fatty acid binding-protein (H-FABP) in the postmortem diagnosis of myocardial ischemia was explored.@*METHODS@#The changes of H-FABP staining in normal, infarcted and suspected ischemia of myocardial cells were studied by immunohistochemistry.@*RESULTS@#There was no depletion in normal control group, and obvious depletion was observed in myocardial infarcted group. Among 9 suspected myocardial ischemia group, 3 cases showed obvious depletion and 3 cases showed vague depletion for H-FABP, there were obvious depletion of Mb in 4 suspected myocardial ischemia cases and vague depletion in 2 cases for Mb. It is indicated that H-FABP can be used to diagnose early myocardial ischemia.@*CONCLUSION@#H-FABP is quite sensitive and useful for the diagnosis of early myocardial ischemia.


Assuntos
Humanos , Biomarcadores/análise , Proteínas de Transporte/análise , Creatina Quinase/sangue , Ensaio de Imunoadsorção Enzimática , Proteínas de Ligação a Ácido Graxo , Imuno-Histoquímica , Isquemia Miocárdica/metabolismo , Miocárdio/patologia , Mioglobina/metabolismo , Sensibilidade e Especificidade
2.
Chinese Journal of Experimental and Clinical Virology ; (6): 112-115, 2003.
Artigo em Chinês | WPRIM | ID: wpr-250528

RESUMO

<p><b>OBJECTIVE</b>To understand the molecular epidemiologic characteristics of hantavirus Shandong isolate JNL virus strain.</p><p><b>METHODS</b>The complete M and S gene of the JNL virus isolated from Shandong Province was amplified by RT- PCR, and the purified PCR product was cloned into T vector for sequencing.</p><p><b>RESULTS</b>The results revealed that the JNL M segment was 3615 bp in length, encoding 1135 amino acids, and the S segment was 1698 bp encoding 429 amino acids, JNL belongs to HTN virus. The comparison of homology with HTN and SEO types showed that the difference of M and S complete sequences between JNL and all other HTN virus strains reached 20.0%-20.6%, and 15.5%-16.0%, respectively. Phylogenetic tree also showed that the position of JNL is located at a different clade.</p><p><b>CONCLUSIONS</b>HTN virus Shandong local isolate JNL strain is a new specific HTN subtype virus.</p>


Assuntos
Humanos , DNA Viral , Vírus Hantaan , Classificação , Genética , Febre Hemorrágica com Síndrome Renal , Virologia , Filogenia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico
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