Determination of Ketoconazole in Disinfectant by HPLC / 环境与健康杂志

Objective To develop a method to determine the content of ketoconazole in the disinfectant by HPLC. Methods The separation was carried out on an ODS C18 column. Methanol-water (80+20) was used as the mobile phases. The flow rate was 1.00 ml/min, the detection wavelength was 239 nm. Results The linearity was obtained in the range of 1.00-100 ?g/ml for ketoconazole, the average recovery rates were 98.1%-99.8%. The difference in a day and in another day was less than 2%. The detection limit was 1.08 ?g/ml. Conclusion This method is simple, accurate, rapid and can be used as a quantitative analysis method for ketoconazole.