RESUMO
Objective To explore the antibacterial activity of extract of Ginkgo biloba leaves on Porphyromonas gingivalis in vitro ,and to provid pharmacological reference for developing a new type of antibacterial drugs in the treatment of periodontal disease.Methods This experiment was divided into negative control group,imipenem control group and different concentrations and forms of extract of Ginkgo biloba leaves groups.Solvent extraction method was used to extract the extract of Ginkgo biloba leaves, punching method and test tube method were performed to detect the antibacterial activity of extract of Ginkgo biloba leaves in anaerobic environment invitro and compared with Staphylococcusaureus and E.coli.By observing the antibacterial ring diameter and determination of the minimum bacteriostasis concentration (MIC),the antibacterial activities of extract of Ginkgo biloba leaves in vitro were measured.Results In the experiment of bacteriostatic ring,Porphyromonas gingivalis was treated with extract of Ginkgo biloba leaves,Ginkgo biloba leaf tablet and Ginkgo biloba soft capsule concentrate and 1∶4 diluent,the bacteriostatic ring diameters were decreased with the decreasing of the concentration.The maximum bacteriostatic diameter of Ginkgo biloba extract was 1 6.5 mm,and the maximum bacteriostasis diameters of Ginkgo biloba leaf tablet and soft capsule were 15.3 and 14.5 mm,respectively;the bacteriostatic diameter of the exact of Ginkgo biloba leaves was bigger than those of Ginkgo biloba leaf tablet and Ginkgo biloba soft capsule (P 0.05);E.coli and Staphylococcusaureus groups get the same results.When the concentration of extract of Ginkgo biloba leaves was more than 1.95 mg·L-1 ,there was no growth of Porphyromonas gingivalis but E. coli and Staphylococcus aureusa still grew;only the concentrations of exact of Girkgo biloba leaves were more than 6.25 and 12.5 mg· L-1 ,E. coli and Staphylococcus aureus didn’t grow;the bacteriostatic effect of extract of Ginkgo biloba on Porphromonas gingivalis was better than E.coli and Staphylococcus aureus . Conclusion Extract of Ginkgo biloba leaves has antibacterial effect on Porphyromonas gingivalis.
RESUMO
Objective To discuss the anatomy,manufacture,merit and demerit of dorsum pedis flap based on anatomic observation and comprehension using free dorsum pedis flap to repair the coloboma of oromaxillo-facial region soft tissue.Methods The courser and disposition of the skin blood vessel and nerve of dorsum of foot of 22 sides of adult cadaver lower limbs were dissected and observed under naked eye and operation microscope. 2 cases got troubled with oromaxillo-facial region malignant tumor,who were prepared 9.5 cm?7.5 cm and 8 cm?6 cm size of free dorsum pedis flap. The site of blood supply and recipient was anastomosed by blood vessel and nerve,and the coloboma of soft tissue was primary repaired after the operation of oromaxillo-facial region malignant tumor. Results Blood circulation of dorsum pedis flap was provided by dorsalis pedis artery,great saphenous vein and small saphenous vein,and fibular nerve was sensory nerve. During two years of follow-up visit,the two cases whose skin flaps were take were satisfactory with the reparation,and tumor recurrence did not take place. Conclusion The blood supply of free dorsum pedis flap is reliable,and it can become ideal skin flap to repair the soft tissue coloboma of oromaxillo-facial region.