Clinical efficacy of Bobath therapy combined with traditional Chinese medicine fumigation and intradermal acupuncture on shoulder hand syndrome after stroke / 中国基层医药

Objective:To explore the clinical effect of Bobath manipulation combined with traditional Chinese medicine fumigation and intradermal acupuncture on shoulder hand syndrome(SHS) after stroke.Methods:From April 2017 to August 2019, 80 patients with SHS after stroke admitted to the People's Hospital of Deqing County were selected, and they were divided into control group and observation group according to the random digital table method, with 40 cases in each group.The control group was treated by Bobath therapy, and the observation group was treated by Bobath therapy combined with acupuncture(intradermal acupuncture) and traditional Chinese medicine fumigation.After 8 weeks of treatment, the ROM scale, FMA scale, BI scale and VAS scores were used to evaluate the improvement in the mobility of the shoulder and wrist joints, motor function of the upper limbs, quality of life and pain, and the clinical efficacy of the two groups was compared.Results:Compared with before treatment, the ROM scale score of all dimensions of shoulder and wrist mobility, FMA scale score and BI scale score of all patients after treatment increased significantly, and the VAS score decreased significantly, the differences were statistically significant(all P<0.05). Compared with the control group after treatment, the improvement of the indicators mentioned above of the observation group were better[shoulder joint flexion ROM score: (154.83±25.63)points vs.(133.82±22.03)points; shoulder joint abduction ROM score: (152.36±25.68)points vs.(133.35±19.96 )points; shoulder joint external rotation ROM score: (75.87±14.69)points vs.(60.82±16.57 )points; wrist joint palm flexion ROM score: (73.94±14.37)points vs.(57.37±9.47)points; wrist joint back extension ROM score: (60.83±7.61)points vs.(42.27±6.37 )points; FMA scale score: (45.74±6.82)points vs.(34.19±4.07)points; BI scale score: (70.36±12.09)points vs.(58.70±12.53)points; VAS score: (1.05±0.49)points vs.(3.37±1.14)points, t=3.703, 3.715, 3.257, 5.576, 7.964, 3.037, 8.746, 3.153, all P<0.05]. The total effective rate of the observation group was significantly higher than that of the control group[97.5%(39/40) vs.62.5%(25/40), χ 2=15.313, P<0.05)]. Conclusion:The combination of Bobath therapy with traditional Chinese medicine fumigation and intradermal acupuncture can improve the pain degree, joint mobility disorder, upper limb motor function and quality of life of SHS patients, the efficacy is better than single Bobath therapy.The clinical effect is accurate, and it is worthy of further promotion and application.

Diagnosis and treatment progress of peritoneal metastasis in gastric cancer / 肿瘤研究与临床

The prognosis of gastric cancer patients is still poor although big progress has been made in the diagnosis and therapy.Peritoneal metastasis is always considered as the end stage of gastric cancer.The treatments such as cytoreductive surgery,intraoperative peritoneal hyperthermic chemotherapy,combined chemotherapy,molecular targeted therapy and gene therapy are applied to clinical management.However,no standard regimens are recommended.The multidisciplinary and individualized treatment will be put more emphasis in the future.This paper reviews the current status and progress of the diagnosis and treatment of peritoneal metastasis in gastric cancer.

The role of Zuckerkandl's tubercle in revealing recurrent laryngeal nerve / 中华内分泌外科杂志

Objective To analyze the anatomical relationship between Zuckerkandl's tubercle (ZT) and the recurrent laryngeal nerve(RLN),to reduce the incidence of RLN injury risk.Methods 280 patients undergoing total thyroidectomy from Jan.2008 to Jan.2013 were included in our study.A meticulous technipue of excapsular dissection was used to dissect thyroid.ZT's size,classification,and its relationship with RLN were studied and classified.Results A total of 280 thyroid gland lobes were dissected.ZT identified in the left was 94.1％(128/136),right 96.4％(160/166),and bilateral 72.8％(220/302).ZT grades were as the following:Grade 0,left 11.8％(16/136) and right 11.4％(19/166);Grade I,left 30.1％(41/136) and right 25.3％(42/166);Grade II,left 44.1％(60/136) and right 43.4％(72/166);Grade III,left 8.1％(11/136) and right 16.3％(27/166).There was no significant difference on ZT classification between the left side and right side.For ZT with grade I or above,112 cases were at the left side,among which type A was 90.2％(101/112),type B 0.9％(1/112),type C 7.1％(8/112),and type D 1.8％(2/112),and 141 cases were at the right side,among which type A was 92.9 ％ (131/141),type B 0.7％(1/141),type C 5.0％(7/141),and type D 1.4％(2/141).There was no significant difference between the left side and the right side in terms of type.Type A of ZT was the most common type.Conclusion As an important anatomic landmark,ZT is essential for locating and dissecting RLN during thyroid surgery,however,due to its complex anatomical relationship with adjacent organs and the variability of RLN and its branches here,from which to reveal RLN has the possibility of increasing the injury risk.

Methods for exposure of recurrent laryngeal nerve in thyroid surgery / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To evaluate different methods to explose recurrent laryngeal nerve (RLN) based on the location of thyroid diseses and anatomic path of the RLN, to avoid the RLN damage in thyroid surgery.</p><p><b>METHODS</b>A total of 755 cases underwent total lobectomy was studied retrospectively. RLN was explosed in each case.</p><p><b>RESULTS</b>A total of 963 RLN was exposed in 755 cases, among those 658 RLN were exposed by lateral approach, 106 by inferior approach, and 199 by superior approach. It was showed that 694 RLN traveled deep to the inferior throid artery and 119 superficial to the artery, 98 through between two branches of the artery, and 62 with the cross of the nerve branches and the artery branches. Before entering larynx, 578 RLN gave off branches and 385 had no branches. Non-recurrent laryngeal nerves were found in 2 cases. There were 6 cases who presented with hoarseness after thyroidectomy and undergoing reexploration, among them RLN were legated in 4 cases and severed in 2 cases.</p><p><b>CONCLUSIONS</b>The anatomic relation of RLN is relatively complicated. Lateral, inferior or superior aproach may be used for exposure of RLN to decrease risks of injury to the nerve.</p>

Diagnostic value of fetal single ventricle types and accompanied malformations by echocardiography / 中华超声影像学杂志

Objective To improve detection rate and diagnostic accuracy of single ventricle(SV) in obstetric fetal echocardiography,to investigate the common types and complicated malformations of SV in the fetus,and to summarize the differential announcements in diagnosing fetal SV.Methods In 345 fetal hearts which were diagnosed as congenital heart disease by fetal echocardiography in our hospital,73 cases diagnosed as SV,including 3 cases appeared as ones of twins,were included in this study.Systemic scanning and multiple-views fetal echocardiography were used to examine these enrolled fetuses.Results In all 73 SV eases,3 cases were diagnosed as simple SV,the others were diagnosed as SV accompanied with other abnormalities,among them 44 cases accompanied with single atrium,18 cases with single atrium and persistent truncus arteriosus,2 cases with pulmonary atresia,20 cases with pulmonary artery stenosis,4 cases with partial atrioventricular septal defect,3 eases with aorta dysplasia or aortic valve dysplasia.SV types were classified as 24.7％ in type A,13.7％ in type B,46.6％ in type C and 15.0％ in type D respectively.68.2％ of the cases were diagnosed with aortic D-transposition,and 45.2 ％ with common inlet,42.5 ％ with single inlet and 12.3 ％ with double inlet respectively.42 SV cases were executed termination of pregnancy which 11 cases were confirmed by pathology and the other 31 cases were out of following-up.Conclusions Most cases of fetal SV were accompanied other abnormalities and simple SV was rare.Type C in which ventricular structure was combined with left and right ventricle was the most common type.To avoid the false diagnosis,much attention must be paid to distinguish big papillary muscle and abnormal muscle bundle from interventricular septum during ultrasonic examination.

Diagnosis and classification of fetal congenital heart disease by echocardiography / 中华超声影像学杂志

Objective To investigate the common types of fetal cardiac malformations and complicated malformations,and to assess the value of classifying on these types.Methods 3201 pregnant women were undergone with fetal echocardiography (FECG),239 fetuses of them were diagnosed to be suffered with congenital heart disease(CHD),and 8 cases were one of twins with abnormal heart confirmed by FECG.All new-births were examined by echocardiography within half year after their births.Results 155 complex CHD in 239 fetuses were diagnosed by FECG,in them the common malformations were in turn 59 cases with diagnosed univentricular heart,29 cases with double outlet right ventricle,19 with atrio ventricular septum defect,12 with tetralogy of Fallot or quinalogy of Fallot,11 with persistent truncus arteriosus,6 with right ventricular dysplasia syndrome,6 cases transposition of the great vessels.100 cases were induced labor,41 of them were comfirmed by pathology.16 fetuses were born,123 cases were being pregnanted or un-followed up.Conclusions Complex and multi-malformation were common in fetal cardiocascular abnormalities.Diagnosing rate of fetal CHD(FCHD) in our enroll fetuses was 7.47 ％,rate of complex CHD vs CHD was 64.85 ％.According different types of FCHD,able to be operated or not after birth,surgery methods,as well as prognosis evaluations,all FCHD cases were classified into three subtypes:curable type,curable palliative type and untreatable type.This newly viewpoint will help pregnant women and their family to make reasonable selection.

High expression of antimicrobial peptide Cecropin AD in Escherichia coli by fusion with EDDIE / 生物工程学报

In this study, we efficiently expressed the active antimicrobial peptide (CAD), which fused with the site-mutated coat protein (EDDIE) of the classical swine fever virus, in Escherichia coli. First, we obtained the e-cad fusion gene from the CAD gene and the EDDIE gene using overlapping PCR. Then to get the recombinant expression vector (pETED), the e-cad fusion gene was cloned into the pET30a vector by a site-directed homologous recombination technique. The EDDIE-CAD fusion protein expressed in E. coli as inclusion bodies, and its yield was more than 40% of total bacterial proteins. After renaturated in vitro and self-cleavage of the fusion protein, we obtained the antimicrobial peptide Cecropin AD. Antimicrobial experiments showed that the Cecropin AD efficiently inhibited the growth of G+ and G- bacteria, but it weakly inhibited the growth of Saccharomyces. This method provides an excellent way for high expression of antimicrobial peptides when fused with EDDIE.

Detection of differently expressed genes in human ovarian carcinoma by cDNA Microarray / 解放军医学杂志

Objective To investigate the differently expressed genes in human ovarian carcinoma, and to reveal the molecular mechanism of the cancerous development. Methods The specimens of human ovarian serous adenocarcinoma tissues in stage III, and of normal human ovarian tissues as control were excised during surgery for present study. Clinical stages were determined by the Federation International of Gynecology and Obstetrids (FIGO). Total RNA was isolated from human ovarian tissues, and cDNA probe was labeled and purified. The amount of radioactivity incorporated into the cDNA probes was checked by a scintillation counter. The profiles of gene expression were compared between carcinomas and normal ovarian tissues by cDNA microarray which contained 588 genes totally. Results Forty-four differentially expressed genes were identified from the 588 genes which were from ovarian carcinoma and normal ovarian tissues and compared with cDNA expression array and analyzed by AtlasImage 1.01 software. 11 of the 44 genes were up-regulated in ovarian carcinoma tissues (including c-erbB2, neu, c-fos, c-myc proto-oncogenes, HER2 receptor, and so on), and the other 33 genes were down-regulated (including RAR, MMP18, MMP19, p21, DNA-PK, and so on). Conclusion The gene expressions in human ovarian carcinoma have been detected in present study. It is the differently expressed genes that help us to disclose the potential molecular mechanisms of the developmental process of human ovarian carcinogenesis. The differently expressed genes may provide a useful hallmark for the early diagnosis of human ovarian carcinoma.

Clinical features and therapy of the elderly hepatic portal cholangiocarcinoma / 中国癌症杂志

Background and purpose:The morbidity of the elderly patients with hepatic portal cholangiocarcinoma is rising. Due to the characteristics of pathology,physiology and anatomy of the disease, most of the patients with cancer are not resectable, the main treatment consists of the diverting drainage and postoperative chemoradiation.We studied the clinical features and the effective therapeutic method for the elderly patients with hepatic portal cholangiocarcinoma. Methods:A retrospective analysis was performed in 28 cases of the elderly patients with hepatic portal cholangiocarcinoma who were treated between January 1995 and December 2005 in our hospital.Results:3 cases received surgery, 2 of them survived for over three years, another 3 cases were given operative exploration with 14 months of medium survival time. 22 cases were given internal or external drainage and received radiotherapy after operation, their survival time ranged from 5 months to 40 months.Conclusions:Surgery is the primary therapeutic method for elderly patients with hepatic portal cholangiocarcinoma. Internal or external drainage and radiotherapy could prolong survival time of the patients with unresectabke disease.

Hepatocyte growth factor and c-met's expression in ovarian tumor cell / 肿瘤研究与临床

Objective To approach the relationship of the expression of hepatocyte growth factor (HGF)/c-met and clinical characteristics of patients with ovarian tumor. Methods Immunohistochemistry (SABC)was used to detect the expressions of HGF and c-met in different pathology group with ovarian cancer. Results HGF positive cells are chromatosised to yellow and take strong expression in the ovarian cancer tissue. C-met takes strong expression in epithelial cell and amply-chromatosis in interstitial cell. HGF and c-met have significant deviation in ovarian tumors, commissurotome tumors and benign tumors (P

Identification of differentially expressed genes involved in diabetes-induced embryopathy by cDNA microarray / 解放军医学杂志

Objective Our purpose in this study is to investigate genes involved in the development of diabetes-induced embryonic malformations. Methods Two groups of 70-90 day old Sprague-Dawley rats were employed in our study: group 1 was normal control rats receiving a normal diet (n=3); group 2 consisted of experimentally-induced diabetic rats by intravenous injection of 65mg/kg of streptozotocin(STZ) on pregnancy day 6 with an attempt to reproduce malformations in embryos (n=3). Embryos were examined on day 12 under light microscopy to look for morphological defect of the neural tube (NTD). Yolk sac cells were harvested from each group and RNA was isolated. Genes expression profiles in yolk sac cells were analyzed using a DNA microarray technique. Results Gene expression patterns were compared in a total of 1200 genes between experimentally-induced diabetic rats and normal control rats, and 79 of genes were found to express differently between the two groups. Forty-two of genes were up-regulated in yolk sac cells of diabetic rats, such as apoptosis related genes BAX, bcl-2, heat shock 70kD protein and glucose-transporter 3; 37 of genes were down-regulated, such as phospholipase A2, insulin-like growth factor II receptor. Conclusion Understanding of differently expressed genes should help us disclose the potential molecular mechanisms underlying the developmental process during diabetes-associated embryonic morphogenesis, and it also might provide a useful tool in rapid diagnosis and prevention of malformation in early gestation stage of diabetic subjects.

Effect of gestrinone on growth and apoptosis in isolated ectopic endometrium cells in vitro / 中华妇产科杂志

Objective To investigate the effects of gestrinone on growth and apoptosis, as well as the expression of phosphatase and tension homologue deleted on chromosome 10(PTEN) in isolated ectopic endometrium cells in vitro and the underlying mechanisms. Methods Ectopic endometrium cells were cultured and exposed to gestrinone of different doses of 0, 10 -6 and 10 -4 mol/L respectively. The inhibition of the cells during 48 hours was determined by methylthiazolyl tetrazolium (MTT) assay, and the cell growth curve was made. Gestrinone was administered to the cells and at 24 hours the morphological changes were observed by transmission electron microscopy and the apoptosis rate, cell cycle and PTEN expression were monitored by flow cytometry (FCM) at the same time. Results Gestrinone at different concentrations could inhibit the growth and proliferation of ectopic endometrium cells in a dose- and time-dependent manner. The inhibition rate of cell growth after exposed to gestrinone for 8,16,24,32,40 and 48 h was 99.6%,87.3%,79.8%,62.3%,51.7% and 44.2% in the 10 -6 mol/L group,and 99.2%,77.1%,69.6%,51.1%,33.7% and 23.6% in the 10 -4 mol/L group (P

ISOLATION AND IDENDTIFICATION OF CYCLIC ?-(1,2)-GLUCANS / 微生物学通报

Rhizobium leguminosarum biov.trifolii H954 has been fermented for eight days on the GMS medium.Culture supernatant is extracted by ethanol precipitation,sample is purified by Sephadex G\|50,Sephadex G\|10 and DEAE\|Cellulose chromatoraphy.Gas chromatography(GC), 13 C nuclear magnetic resonance spectroscopy(NMR)and mass spectrometry(MS) analysis indicated that the sample is cyclic,neutral glucan,and composes of glucose linked solely by 1→2 glucosidic bonds.Cyclic glucan has degrees of polymerization ranging form 17 to 22,with a degree of ploymerization of 19 as the major glucan cycles.

Different concentrations of intracellular calcium in uterine myometrial cells at term and non-term / 中国病理生理杂志

AIM: To investigate different intracellular concentration of Ca 2+ in uterine myometrial cells at term and non-term.METHODS: The living cells suspensions were made to measure intracellular Ca 2+ concentrations after stainned by calcium fluorescent indicator Fluo-3 AM, then examined by laser scanning confocal microscope (LSCM).RESULTS: Intracelluar Ca 2+ showed very stronger red positive signal in myometrial cells at term than that in non-term cells. [Ca 2+ ]i were (35?8.1) nmol/L at non term and (75?7.3) nmol/L at term, which had significant difference compared with each other ( P

THE REGULATION EFFECT OF ALL-TRANS-RETINOIC ACID ON SIGNAL TRANSDUCTION PATHWAY OF GAP JUNCTION GENE CONNEXIN 43 IN HELA CELL LINE / 解放军医学杂志

Cell culture, Fluo-3 AM loading and laser scanning confocal microscope(LSCM), flow cytometer(FCM)and Western blot were employed to detect the regulation effect of retinoic acid on signal transduction pathway of gap junction gene connexin cx43 in HeLa. The results showed that after treated by ATRA , the second intercellular messenger [Ca 2+]i was much higher in HeLa cells(58.16 nmol/L)than in untreated cells(35.73 nmol/L). A detectable and up-regulation of Cx43 in 43kDa protein in HeLa cells increased from 1.9% to 26.3% in RA-treated cells than in untreated cells examined by FCM and Western blot. Immunoblot showed that only the treated cells had phosphorylated Cx43 protein of 43 kDa. The results suggest that the anti-tumor effect of ATRA in HeLa might be due to up-regulation of cx43 gene and its signal transduction pathway which mediates GJIC. The decreased expression of Cx genes, and the disorder and abnormal signal transduction pathway of Cx gene should be responsible for the uncontrolled tumor cell growth.

Effects of methoxychlor on placenta of gravid rats and their progeny / 解放军医学杂志

Objective To investigate the effects of exposure of gravid rats to the persistent organic pollutants (POPs), methoxychlor (MXC), on the placenta and their progeny during gestation periods. Methods According to different dosage levels of MXC [16, 32, 64 and 0 mg/(kg?d)], forty female SD rats aged 3-month were randomly divided into low-dosage group, mid-dosage group, high-dosage group and control group, with 10 animals for each. All the rats received intraperitoneal injection of MXC for 20 days. The influence of MXC on placenta of gravid rats and their progeny were observed in all aspects. Results With the increase in MXC dosage, intense changes were found in the rats, including an inerease in the number of corpus luteum of ovary, the number of nidation, fetal death and merging fetal (P