Establishment and evaluation of the VX2 orthotopic lung cancer rabbit model: a ultra-minimal invasive percutaneous puncture inoculation method

The purpose of the present work is to establish an ultra-minimal invasive percutaneous puncture inoculation method for a VX2 orthotopic lung cancer rabbit model with fewer technical difficulties, lower mortality of rabbits, a higher success rate and a shorter operation time, to evaluate the growth, metastasis and apoptosis of tumor by CT scans, necropsy, histological examination, flow cytometry and immunohistochemistry. The average inoculation time was 10–15 min per rabbit. The tumor-bearing rate was 100%. More than 90% of the tumor-bearing rabbits showed local solitary tumor with 2–10 mm diameters after two weeks post-inoculation, and the rate of chest seeding was only 8.3% (2/24). The tumors diameters increased to 4–16 mm, and irregularly short thorns were observed 3 weeks after inoculation. Five weeks post-inoculation, the liquefaction necrosis and a cavity developed, and the size of tumor grew further. Before natural death, the CT images showed that the tumors spread to the chest. The flow cytometry and immunohistochemistry indicated that there was less apoptosis in VX2 orthotopic lung cancer rabbit model compared to chemotherapy drug treatment group. Minimal invasive percutaneous puncture inoculation is an easy, fast and accurate method to establish the VX2 orthotopic lung cancer rabbit model, an ideal in situ tumor model similar to human malignant tumor growth.

The value of 64-row helical CT multi-phase enhancement scan combined with angiography in the diagnosis of pulmonary mass / 重庆医学

Objective To explore the value of 64-row helical CT multi-phase enhancement scan combined with angiography (CTA)in the diagnosis of pulmonary mass.Methods Two hundred and sixty-five patients with pulmonary mass confirmed by pa-thology were checked,analyzed the CT sign of multi-phase enhancement scan and the blood supply of pulmonary mass displayed by CTA.Results Lung cancer was mainly supplied by bronchial arteries,some by body arteries,the feeding arteries display rate of lung cancer group was significantly higher than that of benign disease group(P 0.05).Enhancement dynamic curves of lung cancer group was differ-ent from benign lesion groups:Lung cancer without obvious enhancement in pulmonary artery phase,CT value increased rapidly in aorta phase,120 s reached peak,and declined slowly in delay phase;CT value of tuberculoma was increased slowly without obvious peak;CT value of inflammatory increased gradually in pulmonary artery phase,90 s reached the peak;hamartoma was no obvious enhancement;Hemangioma enhanced rapidly after strengthening in the pulmonary artery phase,reached the peak at about 15 s,and then decreased slowly.Conclusion 64-row helical CT multi-phase enhancement scan combined with angiography have important clinical value,which can differentiate malignant mass from benign ones.

Comparative study of MRI and pathological findings in senile endometrial polyp / 实用放射学杂志

Objective To analyze the MRI features of senile endometrial polyp and compared MRI features with pathological find-ings,to improve the ability for the diagnosis.Methods The study included 58 cases with senile endometrial polyp proved by diag-nostic curettage or hysterectomy.MRI and pathological findings were analyzed retrospectively.Results Among 58 cases,there were 46 cases with and 12 cases without uterine cavity enlargement.Lesion combined solid portions with cryptomere cystics areas filled in uterine cavity in 35 cases,and MRI showed hypointensity on T1 WI,mixed intensity with hypointensity separation lines on T2 WI, and marked heterogeneous enhancement.The signal of uterine cavity was heterogeneous in 23 cases with hypointensity or isointensi-ty on T1 WI,hyperintensity or isointensity on T2 WI,and no obviously enhancement.Conclusion MRI combined with clinical fea-tures can improve the diagnostic accuracy of senile endometrial polyp.

The pituitary MRI appearances and clinical symptoms in primary hypothyroidism / 重庆医科大学学报

Objective:To report the pituitary sella MRI appearances,clinical symptoms and treatment of primary hypothyroidism in 7 cases,in order to improve the recognition of the pituitary sella MRI appearances,especially the MRI appearances of pituitary caused by the primary hypothyroidism,and to avoid misdiagnosis.Methods:The pituitary sella MRI appearances,clinical symptoms and clinical treatment in the primary hypothyroidism in those 7 cases were analyzed.Results:Features of the pituitary sella MRI appearances in those 7 cases:The pituitary increased in size,which appeared like the tumor in extreme.The MRI appearances of the superior carina were intermediate signal intensity,and the posterior lobe of the pituitary,with an obvious enhancement after Gd-DTPA administration,which was compressed by its anterior lobe,were high signal intensity;other MRI features included enlargement of the sella turcica,invasion of its adjacent bones and opticus nervus,and bad development of sphenoid sinus,etc.With T3,T4 decreasing and TSH markedly increasing that was measured by clinical laboratory examination,7 young children with hypothyroidism visited doctor due to a short stature,but their condition had been improved after an anti-hypothyroidism therapy for 1～ 3 months.The MRI features after treatment:The pituitary reduced largely in size.Conclusion:The primary hypothyroidism,with typical clinical symptoms,stimulates the pituitary that grows abnormally by the feedback regulation;it’s highly recommended to pay attention to clinical symptoms and laboratory examination so as to avoid misdiagnosis when the Cerebral MRI appearances mainly shows that the pituitary enlarges abnormally.

MRI characteristics and clinical manifestation of primary intracranial hypotension syndrome / 重庆医科大学学报

Objective:Toreport the MRI characteristics and clinical manifestation in 3 cases ofprimaryintracranial hypotension syndrome(PIH)and put forward the main points in its diagnosis and examination.Methods:The MRI characteristics and clinical manifestation in 3 cases diagnosed as PIH were retrospectively analyzed.Results:The brain MRI found diffuse pachymeningeal gadolinium enhancement in 2 cases,downward displacement of amygdala cerebelli and narrowing of pontine cistern and cisterna magna in 1 case.In 2 cases by spinal cord MRI,subarachnoid cavity became narrow in multi-series and multi-aspect scanning and it's boundary became unclear;one was located on T5～T7level and the other on T8～T9level;epidural collection of fluid showed strip or comma shape.All the 3 cases had lower CSF pressure(