Determination of aconitine in biological human sample by UPLC-MS/MS / 中国法医学杂志

Objective To develop a simple and fast UPLC-MS/MS method for determination of aconitine in biological human sample. Methods The biological human sample was treated by acetonitrile precipitation, and then analyzed on a UPLC C18(2.1mm×50mm, 1.7μm)column. The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution, at a flow rate of 0.4 mL/min, at 40℃. UPLC-MS/MS was performed in ESI source with MRM mode for quantification. Results The linear range of the concentration were 0.5~500ng/mL in blood and 1~1000ng/mL for aconitine in blood for aconitine (r>0.995). The relative recoveries of aconitine were in the range of 91.3%~110.2%, and the extraction recoveries were in the range of 72.8%~83.5%. The RSDs of intra-days and inter-day were both less than 14%. Conclusion The method is a simple, fast and could be used for determination of aconitine in biological human sample.

Determination of methomyl in human blood by LC/MS / 中国法医学杂志

Objective To develop a sensitive and simple LC/MS method for determination of methomyl in blood samples.Methods Methomyl was extracted from blood by liquid-liquid extraction with ethyl acetate, and then analyzed on a Zorbax SB-C18 (2.1mm×50mm, 5μm) column. The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution, at a lfow rate of 0.5 mL/min, at 40℃. LC-MS was performed in ESI source with MRM mode for quantiifcation.ResultsThe linear range of the concentration were 0.05~2.0μg/mL for methomyl (r>0.995). The relative recoveries of methomyl were in the range of 90%~108%. The RSDs of intra-days and inter-day were both less than 15%.Conclusion The method is a simple, quick, sensitive and could be used for determination of methomyl in blood samples.

Thoughts and exploration on studying pharmacological mechanism of traditional Chinese medicine using network biology approach / 中国中药杂志

The mechanism of synergetic effects of multi-components on multi-targets in traditional Chinese medicine (TCM) is one of the bottlenecks for TCM modernization and internationalization. Network biology approach was developed in recent years and provided an important and novel idea to study the pharmacological mechanism of TCM. This review introduced the mathematical basis of network biology, as well as the feasibility and research idea of using network biology to study the pharmacological mechanism of TCM. Network biology was expected to be further used for analyzing the pharmacological mechanism of TCM, guiding drug development of TCM, inheriting and carrying forward the fundamental principle of TCM.

Advice on how to get further improvements on experimental lessons of science of Chinese Medical Herbs Preparation / 中华医学教育探索杂志

According to the experience of teaching the science of Chinese Medical Herbs Preparation,the author gives some advice such as choosing typical drugs,providing examples and setting up comprehensive and contriving experiments etc,which is worth referring to in teaching es-pecially for those who have less time in experiments.