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AIM: To investigate the effect and mechanism of Astragalus polysaccharide (APS) on the amelioration of hepatic insulin resistance in high fat-fed mouse model.METHODS: C57BL/6J mice (n=26) were divided into three groups randomly: C group (an animal model for control, n=10); IR group ( an animal model of insulin resistance, n=8) and IA group (an animal model in high-fat diet with APS treatment for12 weeks, 700mg?kg-1?d-1, ig). High-fat diet was used to induce the formation of insulin resistant. The parameters and insulin sensitivity of the animals were observed. The pathological features of the liver were presented through microscope and TEM. The expression changes of hepatic GSK3? were measured by Western blotting.RESULTS: In this study, the fat-fed mouse model of insulin resistance was established successfully. The mice in IA group responded to the 12-week APS therapy with a significant decrease in the level of blood glucose, plasma insulin, body weight, hepatic TG/FFA and improved glucose tolerance compared with those in IR group. In addition, the expression and the activity of GSK3? were lower in IA group (vs IR group,P
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Objective To investigate the ameliorative effect and a mechanism of APS on the hepatic steatosis in diabetic KKAy mice.Methods KKAy and C57BL/6J mice were respectively seperated into KK and K+A(n=8)as well as C and C+A(n=10)groups.Blood and hepatic biochemical parameters were observed.Insulin sensitivity was analyzed by OGTT & HOMA-IR.Hepatic pathological changes were presented through microscope and TEM.The expressions of hepatic GSK3? and insulin-induced Ser9GSK3? were performed by Western blot.Results With 8-week APS therapy in K+A group,the levels of blood glucose and hepatic TG and FFA were decreased,insulin sensitivity was improved,the hepatic steatosis was significantly alleviated(P