Progress of reasonable application and control in long QT syndrome associated with drugs / 中国药学杂志

This article has made an review including definition of drug-related long QT interval, clinical features, mechanisms and influencing factors, prevention and treatment of drug-related QT long syndrome, and its clinical research progresses. It is proposed that clinical pharmacists need to focus more attention on QT prolongation drugs. It can prevent acute arrhythmic events, and promote rationality and safety of drug.

Study of screening nephroprotective bioactive substances based on triple-color fluorescence probes in Carthami flos / 中国中药杂志

In this study, an approach based on triple-color fluorescence probes was developed for screening potential nephro-protective bioactive substances. Three fluorescent probes (i. e. FDA, MTR and Hoechst 33342) were used to label HK-2 cells injured by doxorubicin hydrochloride, and cellular fluorescence images were subsequently acquired and analyzed by a cellular-fluorescence image microscopy platform. The established method was applied to screening 53 components of Carthami Flos, and three components C17, C18 and C19 were found to exhibit nephroprotective effects against doxorubicin hydrochloride induced injury on HK-2 cells. Eight compounds (i. e. hydroxysafflor yellow A, 6-hydroxykaempferol-3-O-rutinoside-6-O-glucoside, 6-hydroxykaempferol-3,6-di-O-gluco-side or 6-hydroxykaempferol-6, 7-di-O-glucoside, 6-hydroxykaempferol-3-O-rutinoside, 6-hydroxykaempferol-3-O-glucoside or 6-hydroxykaempferol-7-O-glucoside, rutin, isoquercetin, and kaempferol-3-O-rutinoside) in components C17, C18 and C19 were preliminarily identified by liquid chromatography-mass spectrometry (LC-MS). Isoquercetin, rutin, kaempferol-3-O-rutinoside, and hydroxysafflor yellow A were confirmed by comparing with reference substances, Further study indicated that these four compounds had moderate nephroprotective effects, while isoquercetin showed a significant nephroprotective effect in a dose-dependent manner. These results suggest that isoquercetin, rutin, kaempferol-3-O-rutinoside and hydroxysafflor yellow A might be the nephroprotective bioactive substances in Carthami Flos.

Study on rapid screening and identifying hepatotoxic compounds in toosendan fructus / 中国中药杂志

Fluorescein diacetate-labeled HepG2 cells model and flouresence automatic microscopy screening assay were used for fast screening 23 components from Toosendan Fructus, in which 5 components showed significant toxicity on HepG2 cells. The 10 compounds in the 2 components were tentatively identified with LC-MS(n), and 3 of them (meliasenin B, trichilinin D and 1-O-tigloy-1-O-debenzoylohchinal) were prepared and identified. Further experiments showed that the 3 compounds displayed dose-dependent toxicity on HepG2 cells, suggesting that these compounds in Toosendan Fructus may cause hepatotoxicity.

Establishment and evaluation of a nicotine-treatment and -withdrawal rat model / 第二军医大学学报

Objective: To establish a nicotine-treatment and -withdrawal rat model and to evaluate its characteristics and application through analyzing 3 parameters. Methods: Male Sprague-Dawley rats, aged 10-11 weeks old, were randomly divided into normal saline group(subcutaneous injection of saline [0.5 ml/kg] for 6 weeks), nicotine-treated group (injection with nicotine [0.5 ml/kg, 3 mg/(kg · d)] for 6 weeks), and nicotine-withdrawn group (injection with nicotine [0.5 ml/kg, 3 mg/(kg · d)] for 3 weeks and followed by saline injection for additional 3 weeks). Body weight, food intake, and water intake of animals were recorded during the treatment in 3 groups. The model was evaluated through analyzing body weight, serum parameters and adipose tissue weights. Results: The body weight of rats, as well as the serum levels of triglyceride and insulin, were all decreased after nicotine treatment; the weights of subcutaneous fat, visceral fat and periaortic fat were also decreased. The above indicators increased after withdrawal of nicotine. Conclusion: The established model can be used to study multiple pharmacological effects of nicotine; it can also be used for smoking and smoking cessation related studies.