Study on relationship between vascular endothelial growth factor and tumor burden / 中国免疫学杂志

Objective:To investigate the relationship between vascular endothelial growth factor ( VEGF ) and tumor burden. Methods: 100 cases of healthy volunteers,high risk of malignancy (family history of malignant tumors),without site lesions in patients with malignant tumor after operation,patients with stable disease after treatment,newly diagnosed malignancies were enrolled respectively,and recorded as control group,high-risk group,operation group,stable group and newly diagnosed group. The serum VEGF levels in the groups were quantitatively detected respectively,and the peripheral blood CD4+and CD8+were measured,then the CD4+/CD8+was calculated. The increasing rates and grading of VEGF, the serum VEGF levels and the CD4+/CD8+values were compared. Results: There was a significant difference in serum VEGF classification among the groups (P＜0. 05). The increasing rates of serum VEGF and the serum VEGF levels in the high-risk group,the operation group,the stable group and the newly diagnosed group were much higher than those in the control group (P＜0. 05),of which the CD4+and CD4+/CD8+values were much lower than those in the control group ( P＜0. 05) . The increasing rates of serum VEGF and the levels of serum VEGF in the operation group,the stable group and the newly diagnosed group were much higher than those in the high-risk group (P＜0. 05),of which the CD4+and CD4+/CD8+values were much lower than those in the high-risk group (P＜0. 05). The increasing rates of serum VEGF and the levels of serum VEGF in the stable group and the newly diagnosed group were much higher than those in the operation group (P＜0. 05),of which the CD4+and CD4+/CD8+values were much lower than those in the operation group (P＜0. 05). The increasing rates of serum VEGF and the level of serum VEGF in the newly diagnosed group were much higher than those in the stable group (P＜0. 05),of which the CD4+and CD4+/CD8+value were much lower than those in the stable group (P＜0. 05). The CD4+levels in the stable group and the newly diagnosed group were much lower than those in the control group,high-risk group,operation group (P＜0. 05). In the newly diagnosed group,serum VEGF level was negatively correlated with CD4+/CD8+(r=-0. 578,P＜0. 05). Conclusion: The level of serum VEGF in patients with malignant tumor increase in varying degrees,which also has certain relationships with cellular immune function,and it has reference value to determine the effect of the control of the tumor.

Expression of aquaporin 4 in 1,2-dichloroethane-induced toxic brain edema in rats / 中国职业医学

OBJECTIVE: To explore the effects of aquaporin 4( APQ4) in rat toxic brain edema induced by subacute 1,2-dichloroethane( 1,2-DCE) exposure. METHODS: Thirty-two specific pathogen free healthy adult female SD rats were randomly divided into control( 8 rats),low-dose( 12 rats) and high-dose( 12 rats) groups. The treatment groups were exposed to 1,2-DCE( low-dose: 600 mg / m3; high-dose: 1 800 mg/m3,nose-only) and the control group was exposed to fresh air by dynamic inhalation for 8 hours per day for consecutive 7 days. After exposure,histopathologic changes were examined in the cerebral cortex. Real-time polymerase chain reaction was used to detect the mRNA relative expression of matrix metalloproteinase 2( MMP2),Na-K-Cl cotransporter-1( NKCC1) and AQP4. The Western blotting was used to detect the expression of AQP4 protein in the cerebral cortex. RESULTS: The pathological results showed that the cerebral cortex tissues were loose around the peripheral vessels and the vessels tissue space appeared widen in low-dose exposure group. The pathological change was more serious in high-dose group than low-dose group,with obvious loosen vessels and vacuole. Compared with those of the control group and the low-dose group,the relative expression level of MMP2 mRNA in the high-dose group increased significantly[( 1. 07 ± 0. 41) vs( 1. 56 ± 0. 55),( 1. 21 ± 0. 59) vs( 1. 56 ± 0. 55),P <0. 05],while the the relative expression level of AQP4 mRNA in the high-dose group significantly decreased [( 1. 03 ±0. 25) vs( 0. 81 ± 0. 12),( 1. 00 ± 0. 20) vs( 0. 81 ± 0. 12),P < 0. 05]. The relative expression levels of NKCC1 mRNA in all groups showed no statistical difference [( 1. 03 ± 0. 31) vs( 1. 14 ± 0. 43) vs( 1. 36 ± 0. 50),P > 0. 05]. The relative expression level of AQP4 protein in the high-dose group was lower than that of the control group [( 0. 80 ± 0. 25) vs( 1. 19 ± 0. 42),P < 0. 05]. CONCLUSION: The brain edema induced by subacute inhalation of 1,2-DCE is of mixed types with vasogenic edema as its main symptom. Its pathogenesis is related to the changes of AQP4 expression.

Effect of rapamycin on proliferation, apoptosis and regulation of chemokine receptor CXCR4 in RPMI8226 cells / 中国实验血液学杂志

This study was purposed to investigate the effect of rapamycin on proliferation, apoptosis, cell cycle progression and the regulation of chemokine receptor CXCR4 on RPMI8226 cells. Different concentrations of rapamycin were used to treat the multiple myeloma cell line RPMI8226 for different times. The proliferation of the cells was detected by MTT assay; the apoptosis rate and cell cycle were determined by flow cytometry (FCM); apoptosis of cells was observed by inverted microscopy; the cylin D1, CXCR4 and mTOR mRNA expressions were detected by RT-PCR or FQ-PCR after treating RPMI8226 cells with different concentrations of rapamycin. The results indicated that the rapamycin could inhibit the proliferation of RPMI8226 cells and induce their apoptosis. The cell cycle was arrested at the G(0)/G(1) phase. PCR results showed the down-regulation of mTOR, cyclin D1 and mTOR mRNA expressions after treating RPMI8226 cells with different concentrations of rapamycin for 24 hours. It is concluded that the rapamycin significantly inhibits the growth of RPMI8226 cells in a dose-and time-dependent mannes and induce cell apoptosis. Cell cycle arrests at the G(0)/G(1) phase, may be due to the down-regulation of the mTOR and cyclin D1 expressions. In additions, the down-regulation of CXCR4 mRNA expression is correlated with the reduction of adhesion between myeloma cells and stromal cells.

Expression and clinical value of SHP-1 and c-kit in acute leukemia / 中国实验血液学杂志

The aim of study is to investigate the expression of hematopoietic cell phosphatase (SHP-1) gene and c-kit pro-oncogene in acute leukemia (AL) and its impact on prognosis in AL. Semi-quantity reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of SHP-1 mRNA and c-kit mRNA in 60 AL patients and 33 normal controls (NC). The results showed that the positive rates of SHP-1 expression from high to low level were found orderly in complete remission group, newly diagnosed group and relapsed group, there was significance difference between each group and NC group (P < 0.05). The positive rates of c-kit expression were opposite order in each groups as compared with SHP-1. there was also significance difference between each group and NC group (P < 0.05). The positive rate of SHP-1 and c-kit expressions in AML was higher than that in ALL (P < 0.05), there was negative correlation between expressions of SHP-1 and c-kit (r = -0.502, P < 0.05); The difference between the complete remission rate in SHP-1 positive and in SHP-1 negative patients from 30 newly diagnosed AML patients was significant (P < 0.05), the same result was found between c-kit(+) complete remission and c-kit(-) complete remission. It is concluded that SHP-1 gene is a potentially anti-oncogene and inhibits the growing of tumor by negatively modulating c-kit gene. Simultaneous detection of SHP-1 and c-kit gene may act as a factor for predicting prognosis in AL.