Effect of operative time on prognosis in patients with complicated ocular trauma undergoing vitrectomy / 眼科新进展

Objective To explore the effects of operative time on the prognosis in patients with complicated ocular trauma undergoing vitrectomy.Methods Clinical data of 120 patients (128 eyes) with complicated ocular trauma from May 2012 to May 2017 in our hospital were retrospectively analyzed.Then all the subjects were divided into five groups according to the different operative time of vitrectomy,and the functioncure rate was compared in the 5 groups.Results There were 6 patients (6 eyes) receiving operation on day 0 to 5 after the injury,and the functional cure rate was 66.67％,48 patient (50 eyes) receiving operation on day 6 to 10 after the injury,followed by 32 patients (34 eyes) being functional cured with the curative rate of 68.00％,30 patient (34 eyes) receiving operation on day 11 to 15 after the injury,followed by 24patients (26 eyes) being functionally cured with the curative rate of 76.47％,18 patient (20 eyes) receiving operation on day 16 to 20 after the injury,followed by 8 patients (8eyes) being functionally cured with the curative rate of 40.00％,10 patient (10 eyes)receiving operation on day 21 to 25 after the injury,followed by 2 patients (2 eyes) being functionally cured with the curative rate of 20.00％,8 patient (8 eyes) receiving operationover 25 days after the injury,followed by 2 patients (2 eyes) being functional cured with the curative rate of 25.00％,which suggested that patients suffered the injury with 11 days to 15 days had the highest function-cure rate (all P ＜0.05).Conclusion The operative time does matter,which can affect the cure rate of patients with complicated ocular trauma undergoing vitrectomy,and the most suitable time of ocular trauma surgery is 11-15 days after the injury.

Effects 'of β3 adrenoceptors on the contractility of rat thoracic aorta smooth muscle and the mechanism / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To observe the effect of β₃adrenoceptors (β₃-AR) activation on rat thoracic aorta smooth muscle contractility and the possible related mechanism.</p><p><b>METHODS</b>The endothelium removed thoracic aorta was pre-contracted with 30 mmol/L KCl physiological saline solution (PSS). Then the tension of the thoracic aorta was recorded in presence of BRL37344 (BRL) to determine the action of β₃-AR. The tension of the thoracic aorta was also recorded in the presence of Propranolol (PRA), SR59230A (SR), L-NNA, H-89 and Iberiotoxin (IBTX) respectively to reveal the underling mechanism of β₃-AR activation on rat vascular smooth muscle. Immunohistochemistry was adopted to confirm the existence and the distribution of β₃-AR in rat thoracic aorta.</p><p><b>RESULTS</b>The results showed that: (1) The thoracic aorta was relaxed by β₃-AR activation, with a relaxation percentage of (10.59 ± 0.79). (2) β₃-AR was expressed in both endothelial and smooth muscle layer in thoracic aorta sections of rats. (3) PRA did not block the effect of BRL on the thoracic aorta. The relaxation actions of BRL could be antagonized by pre-incubating the thoracic aorta with SR. (4) L-NNA (a NOS inhibitor) and H-89 (a PKA inhibitor) reversed the relaxation effect of BRL on vascular smooth muscle. (5) The effect of BRL was decreased after application of Ibriotoxin (IBTX), a large conductance calcium dependent potassium channel blocker.</p><p><b>CONCLUSION</b>The results confirmed that activation of β₃-AR led to relaxation of thoracic aorta smooth muscle. The relaxation action of β₃-AR on smooth muscle of rat thoracic aorta was related to activation of NOS and PKA signaling pathway. Large conductance Ca²⁺-K⁺ channels were involved in the relaxation action of β₃-AR activation on rat thoracic aorta smooth muscle.</p>

Experimental study of evodiamine on reversing multidrug resistance of K562/Adr cells / 中草药

Objective: To study the effect of evodiamine (EVO) on the proliferation, cell cycle, and multidrug resistance (MDR) of leukemia K562 and K562/Adr cells. Methods: The effect on the proliferation was detected by CCK-8 kit and/or daunorubicin (DNR). The resistance index (RI) and reversal fold (RF) were calculated. The effect of EVO on cell cycle was tested by flow cytometry; Flow cytometry was used to check the fluorescence intensity of DNR. The expression of MDR1 gene in leukemia K562 and K562/Adr cells was detected by q-PCR. The expression of MDR1 and BCRP proteins in leukemia K562 and K562/Adr cells were detected by Western blotting. Results: CCK-8 test results showed that the proliferation of K562 and K562/Adr cells induced by EVO and DNR was inhibited in a dose and time dependent manner. The RI of DNR on K562/Adr cells was 30.54, the RI of EVO on K562/Adr cells was 19.09|, compared with K562 cells; The RF of DNR + EVO on K562/Adr cells was 12.07.The expression of BCRP and MDR1 protein of K562/Adr cells was significantly decreased in K562/Adr cells induced by EVO and DNR. Conclusion: Therefore EVO can effectively reverse the DNR resistance in K562/Adr cells, which may be related to reduce the expression of MDR1 on cell membrane.

Changes of left ventricular function in cirrhotic patients and their correlation with the model for end-stage liver disease score / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the changes of left ventricular structure and function in patients with liver cirrhosis and their correlation with the model for end-stage liver disease (MELD) score.</p><p><b>METHODS</b>A total of 89 cirrhotic patients admitted between June, 2012 and June, 2014 and 30 healthy control subjects were enrolled in the study. According to MELD score, the cirrhotic patients were divided into 3 groups with MELD scores ≤9, between 10 and 19, and ≥20. The parameters of the left ventricle in resting state were measured using Doppler echocardiography, including left ventricular end systolic diameter (LVESD), left ventricular end diastolic diameter (LVEDD), interventricular septal thickness (IVST), left ventricular posterior wall thickness (LVPWT), left atrial diameter (LAD), ejection fraction (LVEF), cardiac output (CO), mitral flow velocity, and E wave deceleration time (DT), and evaluated their relationship with MELD score.</p><p><b>RESULTS</b>Compared with the control subjects, the cirrhotic patients showed significantly increased LVESD, LVEDD, IVST, LAD, CO and DT but reduced VE/VA ratio (P<0.05 or 0.01). The values of LVESD, LVEDD, IVST, LAD and DT increased gradually with MELD scores (P<0.05 or 0.01). VE/VA ratio was higher in patients with MELD score of 10-19 than in those with MELD score ≤9, and decreased significantly in those with MELD score ≥20. Of the cirrhotic patients, 55% were found to have left atrial enlargement and 44% had a VE/VA ratio ≤1; left atrial enlargement and a VE/VA ratio below 1 were more common in patients with a MELD score ≥20 than in those with lower MELD scores. The LAD, LVEDD and DT were positively correlated with MELD scores (r=0.208, 0.319 and 0.197, respectively; P<0.05 or 0.01).</p><p><b>CONCLUSIONS</b>The patients with liver cirrhosis can have cardiac function deficiency manifested mainly by left ventricular diastolic dysfunction in positive correlation with the severity of liver disease.</p>

Changes of lacrimal film after phacoemulsification / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To observe of cataract phacoemulsification and intraocular lens implantation in patients with postoperative tear film, and to explore its clinical significance. METHODS: A total of 106 patients ( 140 eyes ) undergone phacoemulsification were randomly chosen. Subjective dry foreign body sensation were observed at six nodes of period 1d, 1, 2, 3wk, and 1mo. Corneal fluorescein ( FSC ) , basal tear secretion ( SIT ) and tear film break-up time ( BUT) were used to detect functional changes of the tear film. And the correlation between tear film stability and corneal sensitivity was analyzed. RESULTS: Dry eye cumulative score of postoperative 1d, 1, 2wk was higher than preoperative ( t= 8. 53, P=0.000;t=6. 27, P=0. 000; t=9. 02, P=0. 000). There was no significant difference in dry eye cumulative score at postoperative 3wk, 1mo compared with preoperative ( t=1.91, P= 0. 824; t= 1. 27, P= 0. 069). Corneal epithelial fluorescein staining points of postoperative 1d, 1, 2wk were increased compared with preoperative (t=11. 64, P=0. 000;t=9. 61, P=0. 000; t=8. 87, P=0. 001). There was no significant difference in corneal epithelial fluorescein staining points of postoperative 3wk and 1mo compared with preoperative (t=2. 52, P=0. 746; t=1. 16, P=0. 094). Corneal sensitivity detection values of postoperative 1d, 1, 2wk were significantly higher than that of preoperative (t=9.61, P=0.000;t=9.27, P=0.000;t=11.39, P=0.024), and there was no difference postoperative 3wk and 1mo compared with preoperative (t=1. 19, P=0. 562;t=2. 17, P=0. 501). CONCLUSION: Phacoemulsification with intraocular lens implantation will reduce the tear film stability in the short term, but after a long rest will be improved to a certain extent.

Expression of GDNF in rat Sertoli cells cultured in vitro at different temperatures / 中华男科学杂志

<p><b>OBJECTIVE</b>To explore the mechanism of hyperthermia inducing infertility by observing the expression of glial cell line-derived neurotrophic factor (GDNF) in rat Sertoli cells cultured in vitro at different temperatures.</p><p><b>METHODS</b>Using combination enzyme digestion and selective adhesion, we isolated Sertoli cells from male Wistar rats and cultured them in vitro at different temperatures, followed by observation of the changes in their adhesion and morphology and identification by FasL immunohistochemical staining. We divided the Sertoli cells into a control group (35 degrees C) and four experimental groups (36 degrees C, 37 degrees C, 38 degrees C, and 39 degrees C), measured their proliferation by CCK-8, observed their morphology and structure by HE staining, and determined the expression of GDNF by RT-PCR, immunofluorescence and Western blot.</p><p><b>RESULTS</b>Sertoli cells were successfully isolated and in vitro-cultured, with a purity of (95.30 +/- 2.15)% (n = 10). The CCK-8 assay showed that the proliferation of the Sertoli cells was the highest at 36 degrees C, gradually decreasing with the temperature above 36 degrees C, and significantly inhibited at 39 degrees C (P < 0.01). Immunofluorescence revealed the expression of GDNF in the cytoplasm, with the highest fluorescence intensity at 36 degrees C. RT-PCR and Western blot exhibited a decreasing trend of the GDNF expression with the increasing temperature above 36 degrees C. There were statistically significant differences in the expression of GDNF between the control group and the four experimental groups (P < 0.01).</p><p><b>CONCLUSION</b>The proliferation and GDNF expression of in vitro-cultured Sertoli cells differ significantly at different temperatures. At > 36 degrees C, the higher the temperature is, the lower the Sertoli cell proliferation and GDNF expression are. Our findings suggest that high temperature above 36 degrees C suppresses the function of Sertoli cells and may also damage spermatogenesis.</p>

Influences of bone marrow mesenchymal stem cells in patients with acute myeloid leukemia and non-leukemia on HL-60 cells -- a comparison study / 中国实验血液学杂志

This study was aimed to compare the influences of bone marrow mesenchymal stem cells (BMMSCs) from patients with acute myeloid leukemia (AML), AML patients with complete remission (CR) and non-leukemia patients on HL-60 cells. The HL-60 cells were divided into three groups: group of co-cultivation with BMMSCs of AML patients, group of co-cultivation with BMMSCs of AML patients with CR and group of co-cultivation with BMMSCs of non-leukemia patients. The count of HL-60 cells, the CD11b and survivin expression of HL-60 cells, the cell cycle distribution of the HL-60 cells in 3 groups were compared by flow cytometry, the morphology and differentiation rate of HL-60 cells in 3 groups were observed and compared by microscopy. The results showed that there were no differences in HL-60 cell count at five and seven days, in HL-60 distribution at the G(0)/G(1) phase, in survivin and CD 11b expressions in 3 groups. All cells of 3 groups began to mature, and the differentiation rates in 3 groups were 18.0 +/- 3, 17.0 +/- 1.3 and 19.0 +/- 2.0 respectively, therefore there were no significant differences between the 3 groups (p = 0.23). It is concluded that there is no influence of BMMSCs in 3 groups on the proliferation and differentiation of HL-60 cells.

Comparison between auditory steady-state response and 40 Hz auditory event related potential in the evaluation of hearing thresholds / 法医学杂志

OBJECTIVE@#To compare the results between auditory steady-state response (ASSR) and 40 Hz auditory event related potential (AERP), and explore the accuracy of hearing thresholds by using ASSR and AERP and the clinic forensic value.@*METHODS@#Thirty seven ears were tested with pure-tone audiometer, 40Hz AERP and ASSR, respectively. All the volunteers in our study were awake during 40 Hz AERP test and ASSR test.@*RESULTS@#Thresholds acquired with ASSR and 40Hz AERP test had a close correlativity and showed higher than those acquired with PTA test. There was no significant difference between the accuracy of ASSR and 40Hz AERP in estimating pure-tone thresholds.@*CONCLUSION@#After determining the correct value, ASSR can be used directly to evaluate hearing loss objectively.

Establishment of the sweep pattern visual evoked potential system and its application / 法医学杂志

OBJECTIVE@#To establish an acuity inspection system with sweep pattern visual evoked potential (SPVEP) so as to provide the evidence for acuity objective inspection.@*METHODS@#Based on the domestic sweep pattern visual evoked apparatus, sections of hardware were reformed and a manipulation program possessing false random control software was compiled. The SPVEP acuity for the 78 eyes (10 normal eyes, 10 ametropia eyes, 48 prevalence eyes, 10 false ametropia eyes) was estimated with our acuity objective inspection system, then compared with the E visual acuity of those eyes by statistical procedure.@*RESULTS@#There was a close correlation between the SPVEP acuity and E visual acuity for 78 eyes (r2 = 0.946).@*CONCLUSION@#SPVEP acuity inspection system can be applied to estimate objective acuity.

Apoptosis-dependent acute pulmonary injury after intratracheal instillation of angiotensin II / 生理学报

To test the hypothesis that exogenous purified angiotensin II (ANG) might cause apoptosis of alveolar epithelial cells (AECs) and acute lung injury, male Wistar rats were intratracheally instilled with purified ANG (10 mumol/L), ANG plus the caspase inhibitor ZVAD-fmk (60 mumol/L), ANG plus the ANG receptor AT1 antagonist losartan (LOS, 100 mumol/L) or sterile phosphate-buffered saline (PBS) vehicle alone. Six or 20 h later, the lungs were lavaged in situ for determination of bronchoalveolar lavage (BAL) fluid content of hemoglobin (Hb) and fluorescent (BODIPY)-albumin, a bolus of which was injected intravenously 15 min prior to BAL. Terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) revealed that instillation of ANG, but not PBS alone, increased labeling of fragmented DNA in bronchiolar epithelial cells and in AECs (P<0.05) at 6 h post-ANG. Increased TUNEL was abrogated by concurrent instillation of ZVAD-fmk or LOS. Significant increased numbers of caspase-positive cells were observed by anti-caspase 3 immunolabeling after instillation of ANG (P<0.01); the same doses of LOS or ZVAD-fmk that blocked TUNEL also blocked the activation of caspase 3 (P<0.01). Intratracheal instillation of ANG also remarkably increased BAL BODIPY-albumin (P< 0.01) and Hb (P<0.05), both of which were eliminated by ZVAD-fmk or LOS. These data indicate that exposure of AECs to ANG in vivo is sufficient to induce apoptosis and alveolar epithelial barrier injury mediated by ANG receptor AT1.

Accuracy of age estimation from orthopantomograph using Demirjian's method / 法医学杂志

OBJECTIVE@#Developing teeth are used to assess maturity and estimate age in a number of disciplines. The aim of this study was to determine the accuracy of Demirjian method (Panoramic radiographs were examined and seven mandibular teeth staged according to Demirjian's dental maturity scale) in the forensic clinical medicine.@*METHODS@#Tooth formation was assessed with orthopantomographs in healthy children in dental teaching hospital. There were total 828 children, with 279 boys and 549 girls, aged from 11 to 19 years. The difference between dental and real age was compared and measured, using t-test.@*RESULTS@#The Demirjian method overestimated age in the aged 11-16 years group and had limitations in aged group over 17 years.@*CONCLUSION@#The 95% confidence interval of the mean was least for mean of all developing teeth using Demirjian method (age 11-16 years).