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Background@#At the end of 2019, a novel coronavirus outbreak emerged in Wuhan, China, and its causative organism has been subsequently designated the 2019 novel coronavirus (2019-nCoV). The virus has since rapidly spread to all provinces and autonomous regions of China, and to countries outside of China. Patients who become infected with 2019-nCoV may initially develop mild upper respiratory tract symptoms. However, a significant fraction of these patients goes on to subsequently develop serious lower respiratory disease. The effectiveness of adjunctive glucocorticoid therapy uses in the management of 2019-nCoV infected patients with severe lower respiratory tract infections is not clear, and warrants further investigation.@*Methods@#The present study will be conducted as an open-labelled, randomised controlled trial. We will enrol 48 subjects from Chongqing Public Health Medical Center. Each eligible subject will be assigned to an intervention group (methylprednisolone via intravenous injection at a dose of 1-2mg/kg/day for 3 days) or a control group (no glucocorticoid use) randomly, at a 1:1 ratio. Subjects in both groups will be invited for 28 days of follow-up which will be scheduled at 4 consecutive visit points. We will use the clinical improvement rate as our primary endpoint. Secondary endpoints include the timing of clinical improvement after intervention, duration of mechanical ventilation, duration of hospitalization, overall incidence of adverse events, as well as rate of adverse events at each visit, and mortality at 2 and 4 weeks.@*Discussion@#The present coronavirus outbreak is the third serious global coronavirus outbreak in the past two decades. Oral and parenteral glucocorticoids have been used in the management of severe respiratory symptoms in coronavirus-infected patients in the past. However, there remains no definitive evidence in the literature for or against the utilization of systemic glucocorticoids in seriously ill patients with coronavirus-related severe respiratory disease, or indeed in other types of severe respiratory disease. In this study, we hope to discover evidence either supporting or opposing the systemic therapeutic administration of glucocorticoids in severe coronavirus disease 2019 (COVID-19) patients.@*Trial registration@#ClinicalTrials.gov, ChiCTR2000029386, http://www.chictr.org.cn/showproj.aspx?proj=48777.
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Objective To evaluate sanitary quality of drinking water of small centralized water supply in rural areas using fuzzy comprehensive evaluation method.Methods Fifteen rural small centralized water supply facilities in Jinhua City were randomly selected.The health -related indicators were detected,and fuzzy comprehensive evaluation was used to evaluate the test results.Results The qualification rate of fifteen rural small centralized water supply facilities detected was 6. 67%.The qualified rate of the heat-resistant coliform bacteria in all items was 26. 67%,and the qualified rate of total coliforms was 6. 67%.The qualified rate of total numbers of colony,fluorid,chloride,sulfate,total dissolved solids, iron,oxygen consumption,total hardness and nitrate was all 1 00%.Sanitary quality of the water supply facilities was judged by the fuzzy membership evaluation.There were 4 water supply facilities evaluated as level Ⅰ(good),level Ⅱ(qualified ) and level Ⅳ (bad ) respectivley,and 3 water supply facilities evaluated as level Ⅲ (poor ).Fuzzy comprehensive index of sanitary quality was less than 0. 5 .Conclusion There is a risk of security in rural small centralized water supply water of Jinhua City.The low qualified rate of rural small centralized water supply water was due to heat-resistant coliform bacteria and total coliforms.The relevant departments should strengthen the hygiene management. Disinfection equipment should be installed,and standardized disinfection should be promoted to improve water quality.
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Objective To assess the hygienic status of the central air conditioning ventilation system in public places by fuzzy comprehensive evaluation method.Methods According to the comprehensive evaluation method of public place (WS /T 199—2001),a fuzzy comprehensive evaluation method was applied to evaluate central air conditioning ventilation systems in 15 large public places of Jinhua City.Results The qualified rate of central air ventilation system was 53.30%, and the qualified rates of total number of bacteria,the fungi,β-hemolytic streptococcus in air blow and Legionella bacteria in the cooling water all reached 100.00%.At the same time,the qualified rate of bacteria,fungi and dust volume in internal surface of pipe were 93.33%,86.67% and 80.00% respectively.The qualified rate of PM10 was 86.67%. Among 15 central air conditioning ventilation systems,13 systems were evaluated as level Ⅰ,and the other two was as level Ⅱand Ⅳ respectively.TAAlso,14 evaluated units got the fuzzy comprehensive indexes of central air ventilation less than 0.5.Conclusion The hygiene status of central air conditioning ventilation system in Jinhua is acceptable,and the management of hygiene quality should be strengthened.
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Objective To study oxidative damage for occupational lead exposure, the relationship between serum lead and oxidative stress enzymes, and the mechanism of lead poisoning. Methods The lead content in the air was determined by flame atomic absorption spectrophotometric method, the lead concentration in serum was determined by graphite furnace atomic absorption spectrophotometric method. Superoxide dismutase (SOD) and malondialdehyde (MDA) as the effect indicators of oxidative stress were used to analyze the relationship between blood lead and the indicators. Results Five workplaces were monitored. The lead concentration in exposed group (244.27±124.59ug/L) was significantly higher than that in control group (P<0.01). The SOD activity in exposure group was 61.27±6.97KU/L not significantly different from that in control group (P>0.05), while MDA concetration in exposure group (9.42±3.89mmol/L) was significantly higher than in control group (P< 0.01). There was positive correlation between serum MDA and blood lead concentration (r = 0.3, P < 0.01) . The effects of smoking and drinking on the SOD activity and MDA content were not statistically significant. Conclusion Occupational lead exposure increases the blood lead level. It is inconsistent between the changes of lead concentration in workplace air and in blood lead. Blood lead is a sensitive indicator as the lead internal exposure. The higher blood lead level is, the higher the SOD activity and the MDA concentration, the more seriousthe oxidative damage is.
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<p><b>OBJECTIVE</b>To construct antisense c-Jun N-terminal kinase 1 (JNK1) eukaryotic fluorescent expressing vector and JNK1-/- human embryo lung fibroblasts cell line.</p><p><b>METHODS</b>Trizol reagent was used to extract total RNA in HELF. The proper primers of JNK1 were chosen and synthesized. RT-PCR and gene recombinant techniques were used to construct the fragment of JNK1. After purification, the PCR products were cut, and JNK1 were inserted reversely into eukaryotic fluorescent expressing vector pEGFP-C1. Enzyme-cutting and DNA auto-sequencing were used to prove the successful construction of JNK1 eukaryotic expressing vector. Then plasmids were extracted and transfected into HELF cells and screen by G418 24 h later. Monoclone was chosen and cultured. Fluorescent imaging and Western blot were used to identify the JNK1-/- HELF cell line.</p><p><b>RESULTS</b>Sequence analysis of pEGFP-C1-as JNK1 plasmids was same as expected. The expression level of JNK1 was inhibited markedly.</p><p><b>CONCLUSION</b>Construction of antisense JNK1 eukaryotic fluorescent expressing vectors and JNK1-/- HELF cell line is successful.</p>