Effect of Sinisan on Macrophage Polarization of RAW264.7 Cells Induced by Lipopolysaccharide / 中国实验方剂学杂志

Objective:To investigate the regulatory effect of Sinisan(SNS) on the polarization of RAW264.7 macrophages induced by lipopolysaccharide (LPS). Method:RAW264.7 cells stimulated by LPS were used as the in vitro model. The cells were intervened with the different concentrations of SNS in advance. The effects of different concentrations of SNS on the proliferation of RAW264.7 cells were detected by methyl thiazolyl tetrazolium (MTT) colorimetry. The degree of cell differentiation was detected by enzyme-linked immuno sorbent assay(ELISA) method. The contents of M1 polarization factors tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β) and M2 polarization factors interleukin-10 (IL-10) in cell culture supernatant were detected by ELISA method, mRNA levels of M1 polarization factors TNF-α, IL-6 and M2 polarization factors IL-10, arginase-1 (Arg-1) were detected by Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR) method. Result:SNS had no effect on the cell viability of RAW264.7 cells, inhibited LPS-induced cell proliferation, decreased LPS-stimulated cell differentiation, down-regulated M1 polarizing factors TNF-α, IL-6, IL-1β release and TNF-α, IL-6 mRNA levels, and increased the release of IL-10 and mRNA levels of IL-10 and Arg-1. Conclusion:SNS inhibits the inflammation of RAW264.7 cells induced by LPS, and its mechanism may be related to the regulation of polarization balance of M1/M2 macrophages.

Effect of health-promoting lifestyle on outcomes of suboptimal health status / 南方医科大学学报

<p><b>OBJECTIVE</b>To determine evaluate the effect of health-promoting lifestyle on the outcomes of suboptimal health status (SHS).</p><p><b>METHODS</b>A prospective population cohort was conducted by consecutively enrolling 5676 college students who took routine health examination from March to May 2013. The participants were assessed for baseline health status and lifestyle and 2972 participants with SHS were followed up for 1.5 years. Exposure was defined as an unhealthy lifestyle. The health-promoting lifestyle was assessed via the Health-promoting Lifestyle Profile (HPLP-II). SHS was evaluated using the medical examination report and Sub-health Measurement Scale V1.0 (SHMS V1.0).</p><p><b>RESULTS</b>Among the 2972 students with SHS, 422 showed recovery of the healthy status at 1.5 year follow-up, 579 showed progression into disease conditions, and 1971 remained in SHS. The participants with recovered health status presented with significant increase of SHMS V1.0 scores by 8.75∓6.95 points compared to the baseline assessment (t=-2.14, P=0.000) in physiological, psychological and social dimensions; they also showed a marked improvement of HPLP-II scores by 14.73 points in 6 dimensions (t=-15.34, P=0.000). Multivariable regression analyses with adjusted demographic variables revealed a significant association between health status and health-promoting lifestyle (P<0.05). Compared with a healthy lifestyle (minimal exposure), a 'poor' lifestyle (the highest level of exposure) was associated with a 30 times higher risk of developing SHS (OR: 30.598, 95% CI: 3.928-238.331), while a 'moderate' lifestyle (a relatively high-level exposure) had a 24 times higher risk of SHS (OR: 23.988, 95%CI: 14.695-39.158), and a suboptimal lifestyle had a nearly 4 times higher risk of SHS (OR: 4.306, 95%CI: 2.767-6.702).</p><p><b>CONCLUSION</b>s SHS may evolve into either a healthy or a disease condition. A unhealthy lifestyle is the important risk factor contributing to the progression of SHS into a disease condition, suggesting the importance of intervention of unhealthy lifestyles in promoting good health.</p>

Associations between health-promoting lifestyle and suboptimal health status in Guangdong: a cross sectional study / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate associations between health-promoting lifestyle and suboptimal health status (SHS) in the population of Guangdong province.</p><p><b>METHODS</b>A cross-sectional survey was conducted in a clustered sample of 24 159 individuals aged 12-80 years from 2012 to 2013. Health-promoting lifestyle was assessed via the Health-Promoting Lifestyle Profile (HPLP-II), and SHS was evaluated using the medical examination report and Sub-health Measurement Scale V1.0 (SHMS V1.0).</p><p><b>RESULTS</b>Of the 24159 participants, subjects with SHS (46.0%) and disease status (35.2%) accounted for a much higher percentage than healthy subjects (18.8%). Regression analyses revealed a significant association between health status and healthy lifestyle (P<0.001). Unhealthy lifestyle was an important risk factor for SHS and disease, especially the former. Compared with the participants with a healthy lifestyle (minimal exposure), after demographic adjustment, subjects with a 'poor' lifestyle (maximal exposure) were at a 43 times higher risk of developing SHS (OR: 42.825, 95% CI: 30.567-59.997), those with a general lifestyle were at a 21 times higher risk of SHS (OR: 21.072, 95%CI: 17.258-25.729), and those with a suboptimal lifestyle had a 4 times higher risk (OR: 4.085, 95%CI: 3.352-4.979). In the general population, the major risk factors for SHS included poor stress management, poor self-actualization, inactive exercise and poor interpersonal relationship.</p><p><b>CONCLUSION</b>s Unhealthy lifestyles are significantly related to an increased risk of SHS. Intervention of unhealthy lifestyles, controlling the risk factors of SHS, and rigorous management of the time window of SHS are necessary to promote the heath status.</p>

Plasma metabonomics of Guifu Dihuang Wan in the treatment of yang deficiency / 南方医科大学学报

<p><b>OBJECTIVE</b>To assess the effect of Guifu Dihuang Wan (GFDHW) in the treatment of yang deficiency and explore the underlying molecular mechanism.</p><p><b>METHODS</b>Sixty-two participants without diseases were randomized into control group (n=31) and experimental group (n=31) and were given lifestyle intervention additional GFDHW treatment for a month. NMR technology was used for metabonomics analysis.</p><p><b>RESULTS</b>Intervention with GFDHW resulted in significantly decreased conversion scores of yang deficiency in the experimental group compared with the control group (P<0.005). The concentrations of lactate, valine, proline, arginine and 3-hydroxybutyrate were increased in the plasma of yang-deficient subjects after lifestyle intervention. GFDHW treatment with lifestyle intervention significantly increased the concentrations of lactate, valine, proline, arginine and 3-hydroxybutyrate and also the levels of alanine, glutamine, alpha glucose, isoleucine, betaine and propylene glycol.</p><p><b>CONCLUSION</b>GFDHW treatment improves yang deficiency possibly by increasing the concentrations of alanine, glutamine, alpha glucose, isoleucine, betaine and propylene glycol and promoting energy metabolism of the body.</p>

Assessment of the reliability and validity of the Sub-health Measurement Scale Version1.0 / 南方医科大学学报

<p><b>OBJECTIVE</b>To assess the reliability and validity of the Sub-health Measurement Scale Version 1.0 (SHMS V1.0).</p><p><b>METHODS</b>A spot trial sampling of 2000 individuals was conducted to study the test-retest reliability, Cronbach alpha coefficient, split-half reliability, contract validity, content validity and criterion-related validity of SHMS V1.0.</p><p><b>RESULTS</b>The spot trial results indicated a test-retest reliability of SHMS V1.0 of 0.644 (P<0.001) with a Cronbach α coefficient of 0.917 and a split-half reliability of 0.831. The results showed a close correlation between each item of SHMS V1.0 and its dimension, but a low correlation between a particular item and other dimensions. The dimension score was significantly correlated to its subscale scores, but not to other subscale scores. The results of factor analysis matched the theoretical conception of SHMS V1.0. The correlation coefficient between SHMS V1.0 and SF-36 was 0.664 (P<0.001).</p><p><b>CONCLUSION</b>SHMS V1.0 has a good reliability and validity, and is a reliable and valid measurement scale for sub-health evaluation.</p>

Differential gene expressions in kidney Yang deficiency in individuals with sub-health status / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the gene expression profiles in kidney Yang deficiency in individuals with sub-health status.</p><p><b>METHODS</b>The subjects identified to have Kidney-yang deficiency and sub-health status were enrolled in this study with healthy subjects as the normal control. Using gene chip technique and MAS software, the gene expression profiles in kidney Yang deficiency in the subjects with sub-health status were analyzed.</p><p><b>RESULTS</b>Characteristic profiles of the differentially expressed genes were found between the kidney Yang deficiency group and normal control group. Among the total of 75 differentially expressed genes, 22 were up-regulated (with a ratio greater than 2) and 53 were down-regulated (with a ratio less than 0.5) in kidney Yang deficiency group.</p><p><b>CONCLUSION</b>Gene chip technique allows rapid and high-throughput screening for gene expression in kidney Yang deficiency and sub-health status, and a initial evidence of kidney Yang deficiency-related genes has been obtained.</p>

Analysis of clinical manifestations of subheath status in a medical college of Guangdong Province / 南方医科大学学报

<p><b>OBJECTIVE</b>To explore the clinical manifestation of subhealth status in teachers and medical staff.</p><p><b>METHODS</b>An on-the-spot investigation was conducted in 891 personnel of a medical college using a self-designed subhealth questionnaire. The diagnostic criteria for subhealth status were formulated on the basis of literature review and expert counseling. The results were analyzed by frequency analysis and multiple-factor logistic analyses.</p><p><b>RESULTS</b>The total subhealth incidence was 57.2 %, and the clinical manifestations included many symptoms of the body, psychology and society. Multiple-factor logistic analysis revealed the most common clinical manifestations of subhealth status including ademonia, weariness, insomnia, waist and back pain, frequent sighs, eye dryness, inattention, irritability, foot and hand coldness, vulnerability to common cold etc.</p><p><b>CONCLUSION</b>The clinical manifestations of sub-health status complex.</p>

Evaluation of the questionnaire for subhealth status survey based on the symptoms in traditional Chinese medicine / 南方医科大学学报

<p><b>OBJECTIVE</b>To evaluate the reliability and validity of the questionnaire we designed for subhealth status survey based on the symptoms described in traditional Chinese medicine.</p><p><b>METHOD</b>By on-the-spot investigation, the recovery rate of the questionnaires was 96.3% and the response rate of the items was 97.2%.</p><p><b>RESULTS</b>Internal consistency reliability coefficient of the questionnaire was high (0.9274-0.9676), and each item was closely associated with its related factors (with Spearman coefficient mostly above 0.6 except for that for bodily symptoms in female). Factor loading was approximately consistent with the structure and content of the questionnaire.</p><p><b>CONCLUSION</b>This subhealth status questionnaire can be reliable and effective.</p>