RESUMO
Aim To explore the mechanism of action of the active ingredients of Callerya nitida var.hirsutissima corresponding to the target gene in the treatment of triple-negative breast cancer(TNBC), using network pharmacology, molecular docking technology and in vitro experimental verification.Methods Based on literature research and combined with database screening, the main active components of Callerya nitida var.hirsutissima and the related targets of TNBC were obtained.Intersection genes were found to construct a protein interaction(PPI)network diagram, and core targets were screened according to the size of the correlation.A core target interaction network model of "Traditional Chinese Medicine-Ingredients-Targets-Disease" was constructed.The intersection targets were analyzed for gene GO function and KEGG pathway enrichment analysis.Finally, molecular docking and in vitro experimental verification of the selected components and the target were carried out.Results A total of 38 active components of Callerya nitida var.hirsutissima were collected, as well as 388 related potential targets, 3 919 TNBC targets, and 277 Callerya nitida var.hirsutissima therapeutic targets for TNBC.It mainly acted on multiple targets such as PIK3R1, PIK3CA, MAPK1, AKT1, SRC, etc.In in vitro experiments, it could be seen that the chloroform fraction of Callerya nitida var.hirsutissima and the monomer compounds luteolin and betulin had certain inhibitory effects on cell proliferation.All groups could inhibit the expression of VEGFA, AKT, PIK3CA, CDK1, CDK4 within the range of administration concentration.Conclusions Based on network pharmacology and molecular docking methods, this study explores the possible targets and signaling pathways of Callerya nitida var.hirsutissima in the treatment of TNBC, and conducts in vitro verification experiments to further verify the prediction of network pharmacology.
RESUMO
The Leguminosae perennial vines of Callerya and Millettia have many species and wide distribution， not only can be used for medicines， but also they have ornamental and insecticidal effects. With increasing demand for Spatholobi Caulis， and the reserves of wild medicinal materials are on the verge of exhaustion， resulting in the increasing number of mixtures and substitutes in the market， which makes it urgent to study the origin of Spatholobi Caulis. By referring to related literature， there are three major origins of Spatholobi Caulis， including Callerya， Millettia and Spatholobus. Callerya is separated from Millettia， they are divided and united for many times， now the official website of Flora of China has accepted the revision of them as two genera. This paper intends to compare the chemical components and pharmacodynamic effects of Callerya and Millettia， aiming to explore the similarities and differences between the two genera， so as to determine the rationality and necessity of separating Callerya from Millettia. After comparing， it was found that the chemical composition and pharmacodynamic effects of the two genera were different， which supported the separation of Callerya from Millettia， and it was not recommended to mix use of them.
RESUMO
@#AIM:To study effect of intermittent exotropia operation in children of different ages. <p>METHODS: Totally 300 cases of children who underwent intermittent exotropia surgery in our hospital from March 2015 to April 2017 were selected as the study subjects. According to the age of children, there were 110 cases in Group A, aged from 3 to 5 years. Group B: 100 cases, ages 6 to 8 years. There were 90 cases in Group C, aged from 9 to 12 years. All three groups of children underwent intermittent exotropia surgery. The clinical efficacy, strabismus before and after operation, fusion function and stereoscopic acuity were compared. <p>RESULTS: After 6wk, the total effective rate of clinical efficacy in Group A was 92.7%, significantly higher than that in Group B(67.0%)and Group C(68.9%,<i>P</i><0.05). Before surgery, no statistically significant differences were found in the levels of strabismus, fusion, and stereoscopic acuity between the three groups(<i>P</i>>0.05). At 6wk postoperatively, the children in the three groups had decreased levels of strabismus, and the stereoscopic acuity and fusion function were significantly improved. In addition, the strabismus and stereoscopic acuity of the children in Group A were significantly lower than those in Group B and Group C, and the fusion function was significantly higher than that in Group B and Group C, with statistically significant differences(<i>P</i><0.05). <p>CONCLUSION: The age of the children with intermittent exotropia is closely related to the recovery and reconstruction of binocular vision.