Effect of panretinal photocoagulation combined with intravitreal Conbercept in the treatment of proliferative diabetic retinopathy with different stages / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To investigate the effectiveness of panretinal photocoagulation(PRP)combined with intravitreal conbercept(IVC)for patients with different stages of proliferative diabetic retinopathy(PDR).METHODS: Retrospective study. The medical records for 100 patients(100 eyes)with PDR treated with PRP combined with IVC from January 2018 to June 2020 were reviewed, including 34 eyes with early PDR(group A), 43 with high-risk PDR(group B), and 23 with fibrovascular PDR(group C). The baseline information, best corrected visual acuity(BCVA), central macular thickness(CMT), the rate of vitrectomy and retinal detachment of the patients in the three groups at 1, 3, 6mo and the last follow-up after combination treatment were observed.RESULTS: The patients were followed up for 14.60±11.64mo(6-52mo), with a mean age of 54.22 ±9.32 years. We found 15 eyes(15.0%)who underwent vitrectomy after the combination treatment. The vitrectomy rates of the three groups were 2.9% in group A, 13.9% in group B, and 34.7% in group C. We found no instances of retinal detachment after the treatments. Most patients demonstrated improved BCVA and CMT values with the treatments.CONCLUSION: PRP combined with IVC is safe and effective in patients with different PDR stages.

Application and progress of Internet+ in medical field / 医疗卫生装备

Objective To explore the application mode and the development direction of Internet + in the medical field.Methods The application and development direction of the existing Internet technologies were explored,including smart wearable devices,telemedicine,M-health,cloud + big data and etc.Results The application of Internet + could contribute to improving health management,treatment mode and patient's experience,and save patient's cost.Conclusion The Internet+ medicine,showing broad prospect,will lead the reform and development of medicine.

Effect of naringin on osteoclast differentiation / 中国中药杂志

<p><b>OBJECTIVE</b>To discuss the effect of Drynariae Rhizoma's naringin on osteoclasts induced by mouse monocyte RAW264.7.</p><p><b>METHOD</b>RAW264.7 cells were induced by 100 μg x L(-1) nuclear factor-κB receptor activator ligand (RANKL) and became mature osteoclasts, which were identified through TRAP specific staining and bone resorption. MTT method was sued to screen and inhibit and the highest concentration of osteoclasts. After being cultured with the screened medium containing naringin for 5 days, positive TRAP cell counting and bone absorption area analysis were adopted to observe the effect of naringin on the formation of osteoclast sells and the bone absorption function. The osteoclast proliferation was measured by flow cytometry. The effects of RANK, TRAP, MMP-9, NFATc1 and C-fos mRNA expressions on nuclear factor-κB were detected by RT-PCR.</p><p><b>RESULT</b>Naringin could inhibit osteoclast differentiation, bone absorption function and proliferation activity of osteoclasts, significantly down-regulate RANK, TRAP, MMP-9 and NFATc1 mRNA expressions in the osteoclast differentiation process, and up-regulate the C-fos mRNA expression.</p><p><b>CONCLUSION</b>Naringin could inhibit osteoclast differentiation, proliferation and bone absorption function. Its mechanism may be achieved by inhibiting the specific gene expression during the osteoclast differentiation process.</p>

Three-dimensional reconstruction study of the displacement of undisplaced femoral neck fractures / 中华外科杂志

<p><b>OBJECTIVE</b>To measure the displacement parameters of femoral head in space through three-dimensional reconstruction so as to reunderstand undisplaced femoral neck fractures.</p><p><b>METHODS</b>The clinical data of 80 undisplaced femoral neck fractures from January 2010 to June 2011 were selected, included Garden I 40 cases (group Garden I) and Garden II 40 cases (group Garden II), bilateral proximal femurs of everyone were scanned by CT and reconstructed by professional software. Registered the normal femur and fracture with mirror model, marked key points in the model and measured the displacement parameters of femoral head. The measurement accuracy of distance and angle were 0.01 mm and 0.01°, respectively. The parameters of femoral head displacement between group Garden I and Garden II were analyzed by independent-sample t-test.</p><p><b>RESULTS</b>Eighty cases on X-ray films were not found rotated displacement. But 24 cases of them (30.0%) showed rotated displacement and rotation direction of the femoral head through three-dimensional reconstruction. Ten cases showed incomplete fractures on X-ray films, but only 3 cases were incomplete fractures with three-dimensional reconstruction. In group Garden I, the femoral head displacement angle was 18° ± 11°, the average displacement distance of femoral head center and deepest point of fovea capitis were (6 ± 3) mm and (10 ± 6) mm respectively. In group Garden II, the femoral head displacement angle was 17° ± 10°, the average displacement distance of femoral head center and deepest point of fovea capitis were (7 ± 5) mm and (13 ± 8) mm respectively. There were not statistical significance of the parameters of femoral head displacement between group Garden I and Garden II (P > 0.05).</p><p><b>CONCLUSIONS</b>Three-dimensional reconstruction and digital measurement is a precise, efficient method for the measurement of femoral head displacement parameters in femoral neck fractures, has important clinical significance in the diagnosis and treatment of the femoral neck fractures. There are certain defects and limitations of the classical Garden classification for undisplaced femoral neck fractures.</p>

Biomechanical properties of peripheral nerve after acellular treatment / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Peripheral nerve injury causes a high rate of disability and a huge economic burden, and is currently one of the serious health problems in the world. The use of nerve grafts plays a vital role in repairing nerve defects. Acellular nerve grafts have been widely used in many experimental models as a peripheral nerve substitute. The purpose of this study was to test the biomechanical properties of acellular nerve grafts.</p><p><b>METHODS</b>Thirty-four fresh sciatic nerves were obtained from 17 adult male Wistar rats (age of 3 months) and randomly assigned to 3 groups: normal control group, nerve segments underwent no treatment and were put in phosphate buffered saline (pH 7.4) and stored at 4°C until further use; physical method group, nerve segments were frozen at -196°C and then thawed at 37°C; and chemical method group, nerve segments were chemically extracted with the detergents Triton X-200, sulfobetaine-10 (SB-10) and sulfobetaine-16 (SB-16). After the acellularization process was completed, the structural changes of in the sciatic nerves in each group were observed by hematoxylin-eosin staining and field emission scanning electron microscopy, then biomechanical properties were tested using a mechanical apparatus (Endura TEC ELF 3200, Bose, Boston, USA).</p><p><b>RESULTS</b>Hematoxylin-eosin staining and field emission scanning electron microscopy demonstrated that the effects of acellularization, demyelination, and integrity of nerve fiber tube of the chemical method were better than that of the physical method. Biomechanical testing showed that peripheral nerve grafts treated with the chemical method resulted in some decreased biomechanical properties (ultimate load, ultimate stress, ultimate strain, and mechanical work to fracture) compared with normal control nerves, but the differences were not statistically significant (P > 0.05).</p><p><b>CONCLUSION</b>Nerve treated with the chemical method may be more appropriate for use in implantation than nerve treated with the physical method.</p>

Stress analysis and biocompatibility research on a cell carrier with silicone rubber membrane / 医用生物力学

Objective To report a self-developed cell carrier made of silicone rubber membrane, and to calculate the strain distributions on the membrane and analyze biocompatibility of the silicone rubber by three dimensional (3D) finite element method (FEM) so as to make a comprehensive evaluation on this silicon rubber membrane and provide theoretical basis for cellular research under stress stimulation. Methods The silicon rubber was made into a membrane with the thickness of 0.1 cm. Based on elastic modulus and Poisson’s ratio of the silicone rubber membrane, 3D FEM was used to simulate the deformation of the membrane induced by the stretch stress. MTT method was used to compare the growing situation of cells cultured on the silicone membranes and in standard plastic plates, respectively. Embedding experiment was also conducted to find out whether the material had biological toxicity or not. ResultsUnder the loading of 0.5%~20% strain, the area with effective strain was in the center of the membrane, accounting for 90% of the total area. There still existed some differences in biocompatibility between the silicon rubber membrane and standard plastic plate, but the material itself showed no toxicity. Conclusions Stress distributions on the surface of this self-developed silicon rubber membrane and its biocompatibility were generally acceptable for cell culture, but its surface should be further improved to fit for cell stress experiment.