RESUMO
Objective To observe the changes of CD4+, CD8+T cell proportions and the level of cell surface programmed death-1 (PD-1) in the peripheral blood mononuclear cells (PBMC) of patients with thyroid-associated ophthalmopathy (TAO), and to explore their roles in the pathogenesis and diagnosis of TAO. Methods The clinical data of 21 TAO patients, 21 Graves disease (GD) patients without ophthalmopathy and 20 healthy volunteers were collected. The proportions of CD4+, CD8+T cells in PBMCs and cell surface PD-1 expressions were tested by flow cytometry and were compared between different groups; and their correlation with disease course, thyroid functions, thyroid associated antibodies and severity of TAO were analyzed. Results (1)The proportions of CD4+, CD8+T cells in PBMCs of TAO patients ([35.9±14.5]% and [22.2±8.4]%, respectively) and GD patients ([33.1±13.0]% and [18.6±9.2]%, respectively) were both significantly higher than those in the healthy volunteers ([17.4±7.4]% and [7.7±4.8]% respectively, both P0.05). Conclusion The aberrant proportions of CD4+, CD8+T cells in PBMCs and cell surface PD-1 expression may participate in the autoimmune process of TAO.
RESUMO
Laggera pterodonta is commonly used for treating influenza in Southwest China, especially in Yunnnan province. The main clinical effects of L. pterodonta include anti-influenza, anti-microbial, anti-inflammatory. To investigate the anti-influenza A (H1N1) virus effect of L. pterodonta, neutralization inhibition and proliferation inhibition tests were performed. MDCK culture method was used to observe the cytopathic effect (CPE) of extracts from L. pterodonta in inhibiting influenza A (H1N1) virus and haemagglutination titre of H1N1 virus in vitro. The culture medium were collected at 24 h, 48 h, 72 h, 96 h, and detected by Real time RT-PCR, in order to compare the effect of different extracts from L. pterodonta on in vitro proliferation of H1N1, virus. The result of neutralization inhibition test showed that hemagglutination titer of ethyl acetate extract were 8 times lower at 72 h; in proliferation inhibition test, hemagglutination titer of ethyl acetate extracts reduced by 2 and 4 times. According to the results of Real time RT-PCR test, the H1N1 inhibition ratio of ethyl acetate extract was 72.5%, while the proliferation inhibition ratio of ethyl acetate extract was 25.3%; as for petroleum ether extracts, the H1N1 inhibition ratio was 60.2%, while the proliferation inhibition ratio was 81.4%. In conclusion, both ethyl acetate extract and petroleum ether extract of L. pterodonta have significant neutralization and direct proliferation inhibition effects on influenza A virus.