Screening of prostaglandin E2 receptor 4 antagonist from active compounds of Baeckea frutescens L. and its anti-rheumatoid arthritis effect in vivo / 中国药科大学学报

@#The objectives of the study were to screen prostaglandin E2 receptor 4 antagonist from active compounds of Baeckea frutescens L. and to explore its anti-rheumatoid arthritis effect.The HEK293T-EP4 cell antagonist screening model was established in vitro. Homogeneous time-resolved fluorescence (HTRF) technique was used to screen the active compounds of Baeckea frutescens L..SPF grade ICR male mice were randomly divided into control group, model group, methotrexate group, and Baeckea frutescens L.compound BF-2 (100, 50 and 25 mg/kg) groups. The collagen-induced arthritis (CIA) mouse model was established in vivo. The swelling volume of the toes of mice was measured, and the pathological examination was analyzed by staining with hematoxylin and eosin.SPF grade ICR mice, male, were randomly divided into control group, BF-2 (100, 50 and 25 mg/kg) group, and aspirin group.The acetic acid-induced body writhing test was observed.The EP4 in vitro antagonist screening model was successfully established.The preliminary screening results found that BF-2, BF-20, BF-11 and BF-12 had strong EP4 antagonistic activity [(102.11 ± 3.45)%, (90.31 ± 3.59)%, (75.72 ± 1.79)% and (76.84 ± 1.64)%], and BF-2 had the strongest antagonistic activity (IC50 = 0.99 ± 0.08 μg/mL).BF-2 could significantly inhibit the toe swelling of CIA mice, and relieve the degradation of articular cartilage matrix and inflammatory cell infiltration.At the same time, compared with the control group, the writhing times of the mice in each dose of BF-2 were significantly reduced.In this study, BF-2 of Baeckea frutescens L.was selected as an EP4 antagonist, which has potential anti-rheumatoid arthritis activity.

Analysis the relationship between gestational diabetes mellitus and pregnancy outcome / 中国基层医药

Objective To evaluate the influence of gestational diabetes mellitus(GDM) on the pregnancy outcome. Methods 84 cases with GDM by the clinical data of patients with GDM were analyzed retrospectively;84 cases were divided into untreated group(A group)42 cases and diet plus insulin treatment group(B group)45 cases, compared 2 groups of pregnancy outcome. Results In B group of pregnancy - induced hypertension, placental abrup-tion, polyhydranmios,when the middle class bleeding, puerperal infection (19.6%, 0%, 0%, 2.0%, 3.9%) were lower than A group (42.9%, 7.1% ,4.8% ,7.1%, 9.5%) (t=2.123,t =2.241,t =2.135,t =2. 312,t =2.309,P <0.05) ;In B group of gestational age(33.3%) was significantly lower than A group(23.8%) (χ2 = 3.95, P <0.05) ; In A group of cesarean section rate (69.0%) was significantly higher than the B group (22.2%) (χ2=4.01, P <0.05) ;in B group of stillbirth group (0%) was significantly lower than A group (9.5 %) (χ2= 7.89, P <0.01) ;In B group of birth weight was higher than A group(t = 2.209, P < 0.05), neonatal complications was lower than A group(χ2 =6.89,χ2 =7.09,χ2 =7.49,χ2=6.99,χ2 =7.29,χ2=7.39,P <0.01). Conclusion Pregnan-cy with diabetes is harmful to mother and infant. Diagnosing and treating the eases earlier can decrease the complica-tions of mother and infant as well as the death rate of perinatal fetus.

Sterilization Effect on On-wall Oxygen Humidifying Inhalation Sets / 中华医院感染学杂志

OBJECTIVE To study the sterilization effect of on-wall oxygen humidifying inhalation sets.METHODS The different parts on on-wall oxygen humidifying inhalation sets were sterilized,then compared the result before and after sterilization.RESULTS The regular percents for bacterial examination of humidifying bottle were 17.14% before sterilization,and 100.00% after sterilization,the regular percents for bacterial examination of metallic guilloche were 34.29% before sterilization and 94.29% after sterilization,the regular percents for bacterial examination of vent tube in humidifying bottle were 8.57% before sterilization,and 97.14% after sterilization.The regular percents for bacterial examination of the different parts of oxygen humidifying inhalation sets after sterilization were higher than before it P

Sterilization Method for On-wall Oxygen Humidifying Inhalation Sets / 中华医院感染学杂志

OBJECTIVE To study the sterilization method for on-wall oxygen humidifying inhalation sets in hospital.METHODS The different parts of on-wall oxygen humidifying inhalation sets were sterilized with Jianzhishu disinfectant and Andofo povidone iodine disinfectant liquid.RESULTS The regular percents for bacterial examination of the different parts of oxygen humidifying inhalation sets after sterilization were higher than before,P

Mifepristone vs.Desogestrel-ethinylestradiol for Patients with Perimenopausal Dysfunctional Uterine Bleeding:Comparison of Curative Effects / 中国药房

0.05). However, incidence of amenorrhea and degree of endometrium atrophy were higher in patients treated with mifepristone than in those with esogestrel-ethinylestradiol(P