Effect of a blended learning mode of sleeping intervention for college freshmen / 中国学校卫生

Objective@#To explore the impact of sleep education programs on freshman sleep time, knowledge, attitudes, behavior and daytime sleepiness, so as to provide a reference for further improving the sleep status of college students.@*Methods@#By using the method of cluster sampling, freshmen were invited from a university in Beijing (288 at baseline, 187 at posttest and 108 at follow up for experimental group (EG); 207 at baseline and 105 at posttest for control group (CG). The sleep education content was embedded into other courses and implemented in a blending learning mode for EG, lasting 10 weeks, while the CG received no intervention. Both groups were assessed using questionnaires at both baseline and 2 weeks after the intervention, and reassessed at 9 months follow up after posttest for EG.@*Results@#After intervention, compared with CG, the weekday and weekend bedtime in EG was 21 min and 17 min earlier than that in CG respectively ( t=-2.78, -2.15, P <0.05). The sleep duration at night on weekday in EG was 19 min longer than that in CG ( t=3.51, P<0.01). In EG, the phenomenon of going to bed with electronic products before sleep, no delay in falling asleep, sleep knowledge, sleep attitude, sleep habit and daytime sleepiness were significantly better than those in CG ( χ 2/t =9.15, 2.82, 5.71, 3.98, 2.41, -4.90, P <0.05). After intervention, comparing with that at baseline, the weekday and weekend bedtime in EG were significantly earlier by 11 min and 17 min respectively ( t=3.50, 3.67, P <0.01), the sleep duration at nights on weekdays and weekend increased by 13 min and 18 min, respectively ( t=-3.01, -3.67, P <0.05), and the daytime sleepiness, going to bed with electronic products before sleep, no delay in falling asleep, sleep knowledge, sleep attitude and sleep habit were significantly improved ( χ 2/t =4.64, 15.19, -2.08, -9.31, -3.28, -2.14, P<0.05). At the 9 months follow up after the posttest, the bedtime on working day was significantly advanced by 8 min ( t =2.00), the sleep duration at night on working day was prolonged by 9 min ( t =-2.15), and the phenomenon of going to bed with electronic products before sleep and sleep knowledge were still significantly improved( χ 2/t =21.50, -6.26)( P <0.05).@*Conclusion@#Sleep education programs embedded in other courses and implemented in a blending learning mode can improve students sleep knowledge, sleep attitude and some habits, and reduce daytime sleepiness.

RYBP activates PARP-1 induced Parthanatos in esophageal squamous cell carcinoma cells and enhances response to YM155 / 西安交通大学学报(医学版)

【Objective】 To explore the role of RYBP in activating PARP-1 dependent Parthanatos and promoting response to YM155 in esophageal squamous cell carcinoma. 【Methods】 CCK-8 and flow cytometry were used to analyze the inhibition ratio and cell death percentage after YM155 treatment in both RYBP overexpression group and control group. Western blotting was used to detect the expression of Parthanatos-related proteins. 【Results】 Compared with control group, RYBP overexpression group showed higher inhibition ratio and cell death percentage after YM155 treatment. Overexpression of RYBP activated PARP-1 with or without YM155 treatment. Besides, after YM155 treatment, KYSE170-RYBP showed more PAR accumulation in the nucleus, AIF translocation from mitochondria to the nucleus than control cells. 【Conclusion】 RYBP can activate PARP-1/PAR/AIF-dependent induced Parthanatos in esophageal squamous cell carcinoma and enhance response to YM155.

Genomics prediction analysis of radiotherapy sensitivity and prognosis of esophageal squamous cell carcinoma: Evidence based on bioinformatics analysis / 西安交通大学学报(医学版)

Objective: To explore the key genes affecting the radiosensitivity and prognosis of esophageal squamous cell carcinoma and reveal the related factors of radiotherapy differences in esophageal squamous cell carcinoma at the genomic level. Methods: We downloaded the related chip data of esophageal squamous cell carcinoma receiving radiation therapy from the gene chip public database Gene Expression Omnibus(GEO) (GSE45670). The limma package was used to identify the differential folds of the genes in the pCR and npCR groups, and the genes related to radiosensitivity were screened and enriched using GSEA analysis. The gene expression profile data and clinical data of esophageal squamous cell carcinoma were downloaded from The Cancer Genome Atlas(TCGA) database, and the difference genes between tumor tissue and normal tissue were identified using the edgeR package. We made a comprehensive analysis of the two databases using the STRING database to construct a protein interaction network, and analyzed patient prognosis data based on the level of core gene expression. Results: GSEA showed that the genes of pCR and npCR in GSE45670 were significantly different in cell cycle, DNA replication and other biological characteristics (nominal P<0.05 and FDR<0.25), and 70 radiosensitivity-related genes were identified. According to edgeR analysis, 4855 differential genes were screened between esophageal squamous cell carcinoma and normal tissues in TCGA. Combining the two databases and the STRING database, a total of 46 genes were associated with esophageal squamous cell carcinoma and radiosensitivity, and could be used as candidate genes. Survival analysis showed that PRIM1 and PRKDC were highly expressed in both tumor tissues and pCR groups, and the prognosis was better(P<0.05). Results: Based on GEO and TCGA databases, 46 genes were associated with the occurrence and radiosensitivity of esophageal squamous cell carcinoma, and 26 genes could be the core genes. PRKDC and PRIM1 were highly expressed in the pCR group and correlated well with the prognosis of the carcinoma.

Effect of bifidobacterium-mediated CTP-NPRL2 on growth and apoptosis of nude mouse renal carcinoma / 重庆医学

Objective To evaluate the effect of bifidobacterium-mediated CTP-NPRL2 on the growth and apoptosis of nude mouse renal carcinoma.Methods Recombinant plasmid pET15b-CTP-NPRL2 was constructed and transfected into bifidobacterium by electroporation,and then the expression of fusion protein CTP-NPRL2 was verified by Western blot.The nude mouse renal carcinoma model was constructed by subcutaneous injection of renal carcinoma cells suspension.The nude mice with renal carcinoma were equally and randomly divided into the observation group and control group(8 mice in each group).The mice in the observation group were treated with bifidobacterium containing recombinant plasmid pET15b-CTP-NPRL2 through tail vein injection,and the mice in the control group were treated with normal saline.All mice were treated once a week for four weeks,and then executed for evaluating the weight of mice and bearing tumors.Finally,the apoptosis of renal carcinoma was detected by TUNEL staining.Results The mass of nude mice was(26.24±1.98) g in the observation group and(23.28±2.17) g in the control group.The mass of bearing tumors was(1.37±0.12) g in the observation group and(1.68±0.18) g in the control group,and the differences were statistically significant(P＜0.05).The TUNEL detection results showed that the apoptosis index of renal carcinoma cell in the observation group(23.27±5.14)％ was significantly higher than that in the control group(10.37±2.58)％,and the difference was statistically significant(P＜0.05).Conclusion Bifidobacterium-mediated CTP-NPRL2 has an inhibitory effect on the renal carcinoma growth of nude mouse,and significantly increases the apoptosis of renal carcinoma cells.

Effects of network use behavior on life satisfaction of adolescent / 中华行为医学与脑科学杂志

Objective To explore the status of adolescent's network usage and its effect on life satisfaction.MethodsTotally 691 teenagers were tested by self-regulation scale,the online identity experiment scale and teenager life satisfaction scale.Results①The scores of urban teenagers were significantly higher than those of rural teenagers in self regulation of network behavior(involved-emotional control:(31.40±0.31) vs (29.33±0.32),network congnition (24.37±0.25) vs (22.69±0.21),offline introspection:(15.89±0.30) vs (14.84±0.26),online self-control:(20.68±0.33) vs (19.25±0.28)) and life satisfaction (173.92±1.55) vs (164.00±1.48)(t=4.642,5.082,2.654,3.349,4.613;P<0.01),but lower in negative intrinsic character(5.37±0.13) vs (5.82±0.14)(t=-2.285,P<0.05).The scores of male adolescents were significantly higher than that of female adolescents in network identity and academic satisfaction(t=2.577,4.095,2.051,2.700,P<0.05,P<0.01),but lower in self regulation of network behavior (t=-3.966,-2.653,-5.059,P<0.01).②There was significantly positive correlation between youth life satisfaction and net-addiction cognition,involved-emotional control,network cognition,offline introspection,online self-control,self-control to network attachment,positive intrinsic character(r=0.164,0.307,0.252,0.386,0.430,0.222,0.404,P<0.01),and negatively correlated with positive extrinsic character and negative intrinsic character(r=-0.126,-0.156,P<0.01).③Positive intrinsic character,online self-control,involved-emotional control,offline introspection,self-control to network attachment could be used to predict life satisfaction,ans could explain 38.9% of total variance (F=87.26,P<0.01).ConclusionThere are significantly differences of self regulation of network behavior,network identity and life satisfaction in different gender,between urban and rural areas.The adolescents with higher self regulation of network behavior and network identity have higher life satisfaction.

Analysis of correlative factors of sterility in males undergoing routine sperm inspection by masturbation / 中南大学学报(医学版)

OBJECTIVE@#To investigate the factors influencing sterility in males undergoing routine sperm inspection by masturbation.@*METHODS@#Scales for demographic data, self-compiled infertility questionnaire, Symptom Checklist-90 (SCL-90) , and sexual life subscale of Olson Marital Quality Questionnaire (ENRICH) were assessed in 220 cases of sterility in males who had undergone sperm examination after ejaculation.@*RESULTS@#The total SCL-90 scores and the factor scores of anxiety, phobia, somatization, obsessive compulsive behavior, interpersonal-sensitivity, hostility, and depression were significantly higher than the norm (P<0.05). The total SCL-90 score of 69 males was higher than 160, implying that 31.36% of the sterile males had negative emotions. The total score was related to wife's attitude, semen collecting room, ejaculation situation, and the general state of sexual life. The ejaculation situation was subjected to a multivariate linear regression model.@*CONCLUSION@#About 1/3 of males with sterility problems undergoing routine semen examination by masturbation have negative emotions such as anxiety, phobia, somatization, and interpersonal sensitivities. The defective ejaculation may be the influential factor at the stage.

The changes of estrogen receptor and pogesterone receptor in the endometria of proliferative phase in polycystic ovary syndrome patients / 中国医师杂志

Objective To explore the changes of estrogen receptor (ER) and progesterone receptor (PR) in the endometria of pro-liferative phase in polycystic ovary syndrome (PCOS) patients. Methods Thirty-two patients who suffered PCOS combined with infertilitas feminis were enrolled in this study, and 20 cases of tubal infertilitas feminis having the corresponding time period were selected as controls. The expressions of estrogen receptor (ER) and progesterone receptor (PR) in the endometria were observed by pathological examination and immunohistochemical staining. Results The expressions of ER and PR in the PCOS group in the glands and interstitium of endometrial a-mong three different periods were not significantly different. The expressions of ER and PR in the glands and interstitium of endometrial a-mong three different periods in the control group were significantly different. There was no statistical difference in the expressions of ER, PR in the glands and interstitium of the early endometria between PCOS group and control group. The expressions of ER in the glands and inter-stitium of the middle endometrial in PCOS group was significantly lower than that of control group. The expressions of ER and PR in the glands and interstitium of the late proliferative endometrial in PCOS group was significantly lower than that of control group. Conclusion ER and PR of endometrial in the PCOS patients decreased. The cyclical of ER and PR in the PCOS patient were irregular.

Glucocorticoid or androgen for autoimmune premature ovary failure in mice / 中南大学学报(医学版)

Objective Using mouse autoimmune premature ovary failure (POF) model to seek theoretical evidence for a possible clinical therapy of autoimmune POF with glucocorticoid (GC) or an-drogen. Methods After autoimmune POF was induced in 60 mice by Pzp3, the mice were randomly as-signed into 3 groups (n=20) : Two groups were treated with GC or androgen and the control group was treated with distilled water. We observed the changes in the sexual cycles of the mice, the serum level of AzpAb, infiltration of cells positively expressing CD45 in the ovary, and pathological alterations of the ovary. Results The sexual cycle of each therapy group was significantly different from that of the control group. The mean serum level of AzpAb of each therapy group was significantly lower than that of the con-trol group, and the mean serum level of AzpAb in the GC group was significantly higher than that of the androgen group. The percentage of growing follicles in the ovary of each therapy group was significantly higher than that of the control group. Ovaries infiltrated by cells positively expressing CD45 of each thera-py group were significantly fewer than those of the control group. Conclusion GC or androgen in mice with autoimmune POF could obviously ameliorate the pathogenetic conditions of the disorder, and both treatments have similar therapeutic efficacy.

Effects of androgen on autoimmune ovary failure of mouse / 基础医学与临床

Objective To identify the effects of androgen on mouse model of autoimmune ovary failure.MethodsFifty mice were randomly divided into five groups as control group,early therapeutic group and its model group,late therapeutic group and its model group.Autoimmune POF was induced in forty mice by Pzp3.Then,the animals were randomly divided into four groups: two were treated with androgen from the 15th and 20th day after immune.The control group was treated with distilled water.The therapeutic efficacy of each treatment was evaluated by the changes in sexual cycles of mice,the serum level of AzpAb,infiltration of cells positively expressing CD45 in the ovary as well as the percentage of growing follicles of the ovary.Results The sexual cycles,serum level of AzpAb,infiltration of CD45 cells in the ovary of each model group were more significant than those in control group;early therapeutic group was less than model group;and serum level of AzpAb in early therapeutic group was lower than that of late therapeutic group.The percentage of growing follicles in the ovary of each model group was lower than that of control group;early therapeutic group was higher than model group and late therapeutic group.Conclusion The use of androgen in mice with autoimmune POF may notably ameliorate the pathogenetic conditions of the disorder,and the treatment during prophase was apparently more effective than that during the advanced stage.

PBL+LBL Double-track Practice in Gynecology and Obstetrics Teaching / 中华医学教育探索杂志

Objective:To investigate the effect of PBL+LBL double-track in long-term-system clinical medicine in Gynecology and Obstetrics.Methods:We divided the students of 2001-seven-year-progrems in random into two groups: trial group practiced PBL+LBL double-track teaching,control group practiced LBL teaching.Results The results showed that although the two groups had no significant difference in examinational total score and in foundational subjects,but the trial group had a significant higher score in comprehend subjects.The students exhibited great enthusiasm to the PBL.And the students learned and absorbed more knowledge of gynecology and obstetrics and literature retrieval.The education method played a comprehensive functional role for the teacher-directed and student-oriented tutorial process,and the teaching effects improved.Meanwhile,the teachers need to be prepared with more knowledge to meet the students query.Conclusions:PBL+LBL double-track teaching method can improve the students'activeness and can culture their ability of self-study;and at the same time the new teaching method can remain the systematicness,profundity and extent of knowledge.It is a better way in clinical education,and is well accepted by both students and teachers as it can greatly improve teaching efficacy.

Design and application of imbedded DBS / 医疗卫生装备

Parkinson's disease is a kind of familiar diseases troubling old people in China.It's recognized internationally that DBS is the best method to control Parkinson's disease.This paper designs a kind of DBS without internal electrical source,which can greatly decrease the treatment cost for Parkinson's disease.The structure,circuit philosophy and experiments on rats are also mentioned.

Remote monitoring system for ECG and CO_2 / 医疗卫生装备

It's necessary to monitor ECG and CO2, the two important physiological parameters. This paper designs a kind of realtime, wireless and concentrated monitoring system for ECG and CO2. With a high cost performance ratio, the system can be applied in the rooms for operation, monitoring, first aid and etc. At the same time, this paper does some researches on the antiinterference method for wireless communication.

Psychiatric Symptoms and Monoamine Neurotransmitter in Serum of PCOS with Infertility Patients / 中国临床心理学杂志

Objective:To explore the concentration of monoamine neurotransmitter in serum and psychiatric symptoms such as anxiety and depression in infertility patients with and without PCOS(No-PCOS),and the relationship between them.Methods:30 infertility with PCOS were selected as experimental group(EG) and 30 infertility patients without PCOS as control group(CG).They were administered Symptom Checklist(SCL-90).Their monoamine neurotransmitter in serum were determined including norepinephrine(NE),3-methoxy-4-hydroxyphenylglycol(MHPG),5-hydroxytryptamine(5-HT),5-hydroxyindoleacetic acid(5-HIAA),dopamine(DA),homovanillic acid(HVA) and dihydroxy-phenyl acetic acid(DOPAC).Results:The findings indicated that the scores in anxiety and depression subscales of SCL-90 were significantly higher in the PCOS group than those in the No-PCOS(P

Prediction of Ovarian Response Using the Antral Follicle Count in in Vitro Fertilization Cycle / 中国医师杂志

Objective To explore the value of predicting ovarian response using the antral follicle count in in vitro fertilization(IVF) cycle. Methods 54 patients in our infertility treatment center were observed, in which 6 patients had poor ovarian response and the other 48 patients had good ovarian response. The clinical data, duration and efficacy of treatment were compared between the two groups. Results The mean number of antral follicles and retrieved oocytes in the third day of menstruation in good ovarian response group was significantly higher than that in poor ovarian response group. The clinical pregnancy rate of the good ovarian response group was better than that of the poor one. Conclusions Monitoring the antral follicle count in both ovaries using vaginal ultrasound may predict ovarian response in IVF in the third day of menstruation.

Study of Chlamydia trachomatis infection on cervical secretion of women with early pregnancy and secondary sterility / 中南大学学报(医学版)

Objective　This study was to investigate the Chlamydia trachomatis (CT) infection rate in cervical secretion of early pregnant and secondary sterility women. Method　CT-DNA in the cervical secretion of the early pregnant, the secondary sterility, and the control women was detected with polymerase chain reaction (PCR). Results　The infection rates of CT in the early pregnant and secondary sterility women （14.28% and 13.33%）were higher than in the control women (3.33%, P<0.05),and there was no significant difference between the former two groups (P>0.05). Conclusion　There are higher infection rates in the early pregnant and secondary sterility women.Detecting CT and treating in the perinatal period are needed in the early pregnant women who want to be pregnant again.