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Objective To investigate hand hygiene status of health care workers (HCWs)in a hospital,and pro-vide guides for improving hand hygiene compliance.Methods Hand hygiene intervention was adopted in a hospital between 2012 and 2014,hand hygiene compliance among HCWs and healthcare-associated infection in patients be-tween August 1 and August 31 of each year were investigated and analyzed statistically.Results In 2102 -2014, hand hygiene compliance rate of HCWs was 59.55%,62.13%,and 65.16% respectively,which showed a increased trend (χ2 =10.018,P =0.002),HAI rate was 2.13%,1 .48% and 1 .06% respectively,which showed a decreased trend (χ2 =82.377,P <0.001 );hand hygiene compliance rate of doctors was lower than nurses (57.97% vs 65.97%);Of different hand hygiene moments,hand hygiene compliance rate was the lowest before touching a pa-tient(41 .32%),and highest after body fluid exposure (76.47%).Conclusion Comprehensive hand hygiene inter-vention in this hospital has achieved preliminary results,improvement of HCWs’hand compliance is helpful for pre-venting HAI.
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Purpose:To study the effect of P14ARF gene on human glioma cell line (U 251) Methods:We transfected P14ARF gene into human malignant glioma cell line with liposomal transfection reagent, we assessed cell growth properties,cell cycle.Results:After successful transfection, the human malignant glioma cell line U 251 proliferation rate was slower. The cell cycle was suppressed at G 1 and G 2.Conclusions:The findings suggest that p14ARF gene may be able to inhibit the growth of glioma cell in vitro.