Effect of polydatin on dynamic changes of excitatory amino acids in cerebrospinal fluid of cerebral hemorrhage rats / 中国中药杂志

<p><b>OBJECTIVE</b>To observe the effects of polydatin on dynamic changes of excitatory amino acids in cerebrospinal fluid and water content of brain tissue of cerebral hemorrhage rats. And to discuss the therapeutic action and mechanisms of polydatin on brain hemorrhagic injured rats.</p><p><b>METHOD</b>A quantitative determination method of Asp and Glu was established by microdialysis-HPLC. The cerebral hemorrhage model in rats was induced by local injection of type VII collagenase. The dynamic changes of Asp and Glu in cerebrospinal fluid were observed on 0, 6, 12, 24, 36, 48, 60, 72, 84, 96, 108 h of cerebral hemorrhage rats, and then the water content of brain tissue was detected.</p><p><b>RESULT</b>The content of Asp and Glu increased rapidly within 24 h after cerebral hemorrhage, and to the highest in 24 h, then decreased gradually. Compared with the cerebral hemorrhage model group, the content of Asp and Glu increased slowly in polydatin group, and there were significant differences in 12-72 h and 6-84 h (P < 0.01, P < 0.05), but there was no significant difference after 84 h and 96 h. Compared with sham group, water content of brain tissue significantly higher in model group, while significantly lower (P < 0.01) in polydatin group.</p><p><b>CONCLUSION</b>Polydatin can inhibit increasing content of Asp and Glu in cerebrospinal fluid dynamics, and significantly inhibit cerebral edema of cerebral hemorrhage rats. It shows that the mechanisms of anti-cerebral hemorrhage injury of polydatin may be related to increasing of excitatory amino acids after cerebral hemorrhage.</p>

Effect of Wamingfen on Eosinophil Apoptosis, Oxygen Free Radical and Serum Hydrocortisone Level in Asthma Guinea Pigs / 中药新药与临床药理

Objective: To explore the effect of Wamingfen on eosinophil apoptosis and oxygen free radicals and to study the influence of hydrocortisone on airway inflammation in asthma guinea pigs. Methods: Guinea pig models of asthma were induced by egg albumin. The effect of Wamingfen on plasma malonyl dialdehyde (MDA), blood glutathione peroxidase (GSH-Px),erythrocytic superoxide dismutase (SOD) activity , serum hydrocortisone level and the percentage of EOS apoptosis in bronchial-alveolar lavage fluid (BALF) were observed. Results: Wamingfen can apparently increase the content of GSH-Px ,SOD and serum hydrocortisone, promote the apoptosis of EOS and reduce the amount of MDA. Conclusion: Wamingfen can effectively increase serum hydrocortisone content, remove the oxygen free radical, promote eosinophil apoptosis and reduce the hyper-reactivity in the inflammatory airway.