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Objective:To establish the method for simultaneous determination of ginsenoside Rb1, calycosin7-O-β-D-glucopyranoside, 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside (stilbene glucoside), ferulic acid, and rhein in Beisheng granules by UPLC-full wavelength scanning method. Methods:The Waters-ACQUITY UPLC HSS T3 (2.1 mm×100 mm, 1.8 μm) chromatographic column was used. The mobile phase was acetonitrile and 0.1% formic acid-water solute, a linear gradient, the flow rate was 0.3 ml/min and full-wavelength scanning with detection wavelength of 190-400 nm. The column temperature was 30 ℃, and sample volumn was 3 μl.Results:Ginsenoside Rb1, calycosin7-O-β-D-glucopyranoside, stilbene glucoside, ferulic acid and rhein were well separated at 203, 260, 320, 316 and 254 nm wavelength, respectively. The linear ranges were 0.013-0.210 μg ( r=0.999 4), 0.027-0.432 μg ( r=0.999 9), 0.038-0.600 μg ( r=0.999 9), 0.005-0.084 μg ( r=0.999 9), 0.003-0.048 μg ( r=0.999 2). The RSDs of precision, repeatability and stability tests were 0.17%-1.73%, 0.40%-1.49%, 0.21%-1.98% ( n=6), respectively. The recovery rates were 92.71%, 92.44%, 96.83%, 105.71% and 102.61% ( n=6), respectively. Conclusions:The method is simple, rapid and accurate, and can be used as a reference for quality control of Beisheng granules.
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Objective To establish quality standards for Kangkeling mixture .Methods Ardisiae Japonicae Herba ,Ste-monae Radix were identified by TLC .The content of bergenin was determined by HPLC .Lichrospher C18 column(5 μm , 4 .6 mm × 250 mm)was used with methanol-water (20:80) as mobile phase .The flow rate was 1 .0 ml/min .Column temper-ature was 30 ℃ and the detection wavelength at 275 nm .Results The linear range of bergenin was in the range of 0 .0648-0 .648 μg (r=0 .9998) .The average recovery was 100 .24％ (RSD=1 .9％ ,n=6) .Conclusion The method is simple ,spe-cific and sensitive .It can be used to control the quality of Kangkeling mixture .
RESUMO
Objective To establish the quality standard for YI SHI MINGMU granules.Methods TLC method was used to identify Lycium barbarum L and Salvia miltiorrhiza.HPLC method was used to quantitatively analyze the concentration of Tanshinol sodium.The analysis was carried out on a column of Kromasil C18(4.6 mm×150 mm,5 μm)with a mobile phase of methanol and 0.5 % acetic acid at the flow rate of 0.4 ml/min.Column temperature was 30 ℃.The detection wavelength was 280 nm.Results The linearity range of Tanshinol sodium was 2.00~60.00 μg/ml ,r2=0.999 7(n=6),with the average recoveries of 105.62%, RSD=1.60%.Conclusion This method is accurate, simple, reliable and reproducible.It can be used for the quality control of YI SHI MINGMU granules.