RESUMO
Objective To establish a quality standard of yinqiao xiaozhen mixture. Methods Preparation of Forsyth-ia,Arctium lappa L.,and Honeysuckle were identified by TLC method.The concentration of baicalin in yinqiao xiaozhen mixture was determined by HPLC method. Results The qualitative identification method can detect Forsythia,Arctium lappa L.,and Honeysuckle.TLC spots were clear.TLC method has strong specificity.The linear range of baicalin was 0.122 5-1.531 2 μg,r=0.999 9,the average sample recovery rate was 99.42%,RSD was 2.19%,respectively. Conclusion The method is simple,ac-curate and repeatable,which can be used for quality control of Qinqiao xiaozhen mixture.
RESUMO
Newborn ovary homeobox gene (NOBOX) is an oocyte-specific homeobox gene that plays a critical role in early folliculogenesis and represents a candidate gene for nonsyndromic ovarian failure. We used in silico approach in combination with rapid amplification of cDNA ends (RACE) to clone the full-length cDNA of NOBOX (GenBank Accession No. FJ587509) from porcine oocytes. It contains 1768 bp nucleotides, with an open reading frame (ORF) of 1419 bp. The putative porcine NOBOX gene encodes 472 amino acids with the molecular weight of 51.08 kD and pI of 5.73. Bioinformatics prediction indicates that this protein contains a cd00086 homeodomain. Real-time PCR analysis showed that the NOBOX gene is expressed in various tissues, oocytes and embryos cells (4-cell, 8-cell, morula and blastocyst) at different expression levels. The expression levels of this gene in heart, kidney and oocytes are higher than that in other tissues, which suggested that the NOBOX protein might play an important role in those tissues. The expression of NOBOX in developmental stages is higher than that in GV-stage oocytes, which suggested that the expression of pNOBOX was enhanced in developmental stages.
Assuntos
Animais , Feminino , Masculino , Sequência de Aminoácidos , Clonagem Molecular , DNA Complementar , Genética , Desenvolvimento Embrionário , Genética , Proteínas de Homeodomínio , Genética , Metabolismo , Dados de Sequência Molecular , Oócitos , Metabolismo , Suínos , Genética , MetabolismoRESUMO
Objective To study the tissue-specificity of ?-carotene-15,15’-monooxygenase(? CMOOX). Method Semi-quantitative RT-PCR and HPLC were used to study the expression of ?CMOOX mRNA and its activity in different tissues of chicken. Results ?CMOOX mRNA was expressed in tissues including heart, liver, spleen, lung, kidney, duodenum, jejunum, ileum, muscle and testis, not expressed in lung. Furthermore, it was expressed in jejunum with the highest level. ?CMOOX activity was found in every tissue, except lung, and highest in duodenum and jejunum. Conclusion The distribution of ?CMOOX in chicken has tissue specificity.