Study on proteomic changes of lung blast injury in mice / 中华急诊医学杂志

Objective:To establish the lung blast injury model in mice, detect the proteomic changes of lung in mice at different time points, and explore the mechanism of lung blast injury.Methods:A total of 60 healthy male C57BL/6 mice were randomly (random number) divided into the control group, 12-h group after thorax blast, 24-h group, 48-h group, 72-h group and 1-week group ( n=10 each group). Experiments were carried out in the animal laboratory of the General Hospital of the Northern Theater Command. The model of lung blast injury in mice was established by using a self-developed precision blast device, and the lung tissue injury situation was evaluated by gross observation and HE staining. The proteins in mouse lung tissue were quantitatively analyzed based on LC-MS/MS proteomic technology, and the differentially expressed proteins were screened. On this basis, bioinformatics tool was used to analyze proteomic changes. Results:After lung blast injury, scattered bleeding spots could be observed on the surface of lung tissue of mice, and the bleeding points were gradually increased with time, showing a patchy distribution, and the symptoms were the most severe at 24 h. The results of HE staining showed that the normal tissue structure of alveoli disappeared at 12 and 24 h under light microscopy with diffuse bleeding in the alveolar cavity, infiltration of a large number of inflammatory cells, increased interstitial exudate, thickened alveolar wall, and collapsed and merged alveolar cavity. A total of 6 861 proteins were identified by LC-MS/MS in lung tissue samples of mice after thorax blast, and 608 differentially expressed proteins were quantified, of which 227, 140, 202, 258 and 71 differential proteins were at 12 h, 24 h, 48 h, 72 h, and 1 week, respectively. According to GO analysis, 130 biological process subtypes including cell adhesion, extracellular matrix tissue and collagen fibril tissue were obtained. Besides, 66 cellular component involving extracellular exosomes, extracellular matrix and cytoplasm were obtained. And 43 molecular functional subclasses such as extracellular matrix structure composition, actin binding and antioxidant activity were obtained. KEGG analysis yielded 24 pathways including ECM-receptor interactions, focal adhesions and PI3K-Akt signaling pathway across the endothelium.Conclusions:Differentially expressed protein combinations are also different at different time points in the early stage after lung blast in mice, and the injury mechanism is complicated. The lung blast injury is the most serious at 12-24 h after blast and produces significant inflammatory response.

Cerebral infarction as the first symptom in acute promyelocytic leukemia: A case report and literature review / 中南大学学报(医学版)

In the clinical settings, disseminated intravascular coagulation (DIC) and complications such as hemorrhage are commonly seen in acute promyelocytic leukemia patients, whereas thrombosis is rarely reported. We reported a case here that the patient presented with cerebral infarction as the first manifestation. During the admission, the patient encountered differentiation syndrome, pulmonary embolism, pulmonary hemorrhage, and myocardial ischemia, as well as bleeding and thrombosis complications. Hence the patient was diagnosed as DIC. After the treatment of blood transfusion instead of anticoagulation, his condition was stable and the remission was completely achieved. The treatment experience provides guides for other patients with similar complications of simultaneous bleeding and thrombosis.

Modeling and analysis of volume conduction based on field-circuit coupling / 生物医学工程学杂志

Numerical simulations of volume conduction can be used to analyze the process of energy transfer and explore the effects of some physical factors on energy transfer efficiency. We analyzed the 3D quasi-static electric field by the finite element method, and developed A 3D coupled field-circuit model of volume conduction basing on the coupling between the circuit and the electric field. The model includes a circuit simulation of the volume conduction to provide direct theoretical guidance for energy transfer optimization design. A field-circuit coupling model with circular cylinder electrodes was established on the platform of the software FEM3.5. Based on this, the effects of electrode cross section area, electrode distance and circuit parameters on the performance of volume conduction system were obtained, which provided a basis for optimized design of energy transfer efficiency.

Pharmaceutical Care in Patients with Non-controlled Asthma:Investigation on 70 Cases / 中国药房

OBJECTIVE: To define the future tasks of pharmaceutical care in asthmatic patients in order to improve pharmaceutical care. METHODS: A questionnaire survey was conducted on 70 outpatients with non-controlled asthma and the data obtained were analyzed statistically. RESULTS: 20.00% of the patients investigated used only controller medications and 10.00% used only reliever medications; 30.00% showed good compliance; 43.28% were able to distinguish controllers from relievers in their own treatment regimens; the proportions of patients who didn't correctly use steroid containing metered-dose inhaler, reliever containing metered-dose inhaler, steroid containing turbuhaler and steroid containing accuhaler were 36.00%, 44.00%, 27.78%, and 23.81%, respectively; 14.29% of the patients kept an asthma diary but none was up to standard; 12.85% owned a peak flow meter; 88.57% judged their treatment outcomes only by symptoms; 51.43% were not aware of any adverse drug reactions of asthmatic medications. CONCLUSION: Asthmatic patients need to be more familiar with their medications. Meanwhile, they need to improve their medication compliance, inhalation technique and self management. Pharmacists should cooperate with other health care professionals and asthmatic patients to improve the asthma control standard in China.