Optimized Extraction of Curcuminoids from Curcuma Longa L.by Orthogonal Designed Method and Its Antioxidant Activity / 医药导报

Objective To investigate the optimal extraction technique and antioxidant activity of curcuminoids from Curcuma longa L. Methods The optimized extraction process of curcuminoids from Curcuma longa L. with ethanol as extracting solvent were studied based on orthogonal test.The effect of each factor such as the ethanol concentration, the amount of solvent, extraction temperature and extraction time were investigated based on a single-factor experiment.The effects of extraction yield of curcuminoids under different processing conditions were compared by HPLC method. Content of curcuminoids and antioxidant activity of the extract were used as comprehensive survey index. Free radical scavenging DPPH method was used to assess the antioxidant effect. And the extraction technique parameters were optimized. Results The condition of optimal extraction technique was: ethanol concentration 60%, amount of solvent 20 folds, extraction time 2 h, extraction temperature 90 ℃. Under these conditions, the extraction yield of curcuminoids was 3. 65%. DPPH free radical scavenging yield of the curcumin extract was 79.79%. Conclusion This optimized technology is simple and have a good reappearing rate, and the extracts of Curcuma longa L.show good antioxidant activity.

Triterpenes from Kalimeris indica / 中国中药杂志

<p><b>OBJECTIVE</b>To study the constituents of Kalimeris indica.</p><p><b>METHOD</b>The constituents were isolated by various chromatographic techniques and their structures were elucidated by their physicochemical properties and the spectral data analysis.</p><p><b>RESULT</b>Eleven compounds were isolated and identified as dammaradienyl acetate(1), friedelin(2), friedelinol(3), beta-amyrin(4), alpha-amyrin(5), lupenyl acetate(6), beta-sitosterol(7), alpha-spinasterol(8), alpha-spinasterone(9), stigmasterol(10), daucosterol(11).</p><p><b>CONCLUSION</b>Compounds 4-6, 8-11 were isolated from the genus Kalimeris for the first time.</p>

Glycosides from Periploca forrestii / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents from n-BuOH fraction of the stems of Periploca forrestii.</p><p><b>METHOD</b>The compounds were separated and purified by various chromatographic techniques and recrystallisation. The physico-chemical properties and spectral data were applied to identify structure of these compounds.</p><p><b>RESULT</b>Six glycosides were isolated and identified as emodin-8-O-beta-D-glucopyranoside (1), physcion-8-O-beta-D-glucopyranoside (2), kaempferol-3-O-beta-D-galactopyranoside (3), kaempferol-3-O-alpha-L-arabinopyranoside (4), quercetin-3-O-beta-D-glucopyranoside (5), quercetin-3-O-alpha-L-arabinopyranoside (6).</p><p><b>CONCLUSION</b>These six compounds were obtained for the first time from this plant.</p>

Chemical constituents and bioactivity of Kalimeris indica / 中国中药杂志

<p><b>OBJECTIVE</b>To study the constituents of Kalimeris indica and its bioactivity.</p><p><b>METHOD</b>The constituents were isolated by various chromatographic techniques and their structures were elucidated by their physicochemical properties and the spectral data analysis. Use the disk diffusion method, see whether a sample can produce inhibition zone and its round size, semi-quantitatively evaluate the samples antimicrobial activity.</p><p><b>RESULT</b>Eight compounds were isolated and identified as succinic acid (1), glycerolmonopalmitate (2), protocatechuic acid (3), uracil (4), syringic acid (5), protocatechuic acid methyl ester (6), esculetin (7), medicagenic acid (8). The result of activity screening indicate that succinic and syringic acid have bacteriostasis to bacillus subtilis.</p><p><b>CONCLUSION</b>All Compounds were isolated from the genus Kalimeris for the first time, succinic acid (1) and syringic acid (5) have bacteriostasis to bacillus subtilis.</p>

Chemical constituents of Periploca forrestii / 中国中药杂志

<p><b>OBJECTIVE</b>To study the constituents of the stems of Periploca forrestii.</p><p><b>METHOD</b>The compounds were separated and purified by silica gel column chromatography, recrystallization and high-performance liquid chromatography. The structures were identified by various spectroscopic methods.</p><p><b>RESULT</b>Nine compounds were isolated and identified as 3-O-acetyloleanolic acid (1), 14-ursen-3-ol-1-one (2), taraxasterol (3), jacoumaric acid (4), periplogenin (5), 2alpha,3beta-dihydroxyursolic acid (6), E-p-hydroxy-cinnamic acid (7), caffeic acid (8), proanthocyanidin A2 (9).</p><p><b>CONCLUSION</b>All compounds except 6 were isolated from this plant for the first time, compound 4, 9 were obtained from the Periploca for the first time.</p>

Studies on chemical constituents of Evodia rutaecarpa / 中国中药杂志

<p><b>OBJECTIVE</b>To study the constituents of Evodia rutaecarpa.</p><p><b>METHOD</b>The constituents were isolated by various chromatographic techniques and the structures were elucidated by their physicochemical properties and the spectral data analysis.</p><p><b>RESULT</b>Eleven compounds were isolated and identified as rutaecarpine (1), dehydroevodiamine (2), limonin (3), wuchuyuamide I (4), evocarpine (5), daucosterol (6), taraxerone (7), 14-ursen-3-ol-1-one (8), trans-caffeic acid methylate (9), methyl coumarate (10), caffeine (11).</p><p><b>CONCLUSION</b>Compounds 7, 8, 10, 11 were isolated from E. rutaecarpa for the first time.</p>

Antagonistic Effects of Extracts from Cultural Mycelium of Cordyceps sinensis on Arrhythmia / 中国药科大学学报

AIM　The purpose is to study the anti-arrhythmic effects of extracts from cultured Cordyceps sinensis. METHODS　Aconitine and barium chloride were used to induce experimental arrhythmia models in rats. RESULTS　The results showed that PE was more potent than other ingredients. Pretreatment with PE by oral administration significantly antagonized arrhythmia induced by aconitine. PE significantly prolonged the onset of arrhythmia, decreased the duration and diminished the degree of severity. And in barium chloride-induced arrhythmia model, PE had some anti-arrhythmia effect，which could diminish the degree of severity effectively to some extent. CONCLUSION　PE is an active component to antagonize arrhythmia induced by aconitine and barium chloride.