Construction of a risk prediction model for bloodstream infection induced by carbapenem-resistant Klebsiella pneumoniae / 中国药房

OBJECTIVE To construct a risk prediction model for bloodstream infection （BSI） induced by carbapenem-resistant Klebsiella pneumoniae （CRKP）. METHODS Retrospective analysis was conducted for clinical data from 253 patients with BSI induced by K. pneumoniae in the First Hospital of Qinhuangdao from January 2019 to June 2022. Patients admitted from January 2019 to December 2021 were selected as the model group （n＝223）， and patients admitted from January 2022 to June 2022 were selected as the validation group （n＝30）. The model group was divided into the CRKP subgroup （n＝56） and the carbapenem- sensitive K. pneumoniae （CSKP） subgroup （n＝167） based on whether CRKP was detected or not. The univariate and multivariate Logistic analyses were performed on basic information such as gender， age and comorbid underlying diseases in two subgroups of patients； independent risk factors were screened for CRKP-induced BSI， and a risk prediction model was constructed. The established model was verified with patients in the validation group as the target. RESULTS Admissioning to intensive care unit （ICU）， use of immunosuppressants， empirical use of carbapenems and empirical use of antibiotics against Gram-positive coccus were independent risk factors of CRKP-induced BSI （ORs were 3.749， 3.074， 2.909， 9.419， 95%CIs were 1.639-8.572， 1.292- 7.312， 1.180-7.717， 2.877-30.840， P＜0.05）. Based on this， a risk prediction model was established with a P value of 0.365. The AUC of the receiver operating characteristic （ROC） curve of the model was 0.848 [95%CI （0.779， 0.916）， P＜0.001]， and the critical score was 6.5. In the validation group， the overall accuracy of the prediction under the model was 86.67%， and the AUC of ROC curve was 0.926 [95%CI （0.809， 1.000]， P＜0.001]. CONCLUSIONS Admission to ICU， use of immunosuppressants， empirical use of carbapenems and empirical use of antibiotics against Gram-positive coccus are independent risk factors of CRKP- induced BSI. The CRKP-induced BSI risk prediction model based on the above factors has good prediction accuracy.

Risk factors of mortality in Klebsiella pneumoniae bloodstream infection and construction of a prediction model for prognosis of patients / 中华临床感染病杂志

Objective:To explore the risk factors of mortality in patients with Klebsiella pneumoniae bloodstream infection, and to construct a predictive model. Methods:The clinical data of 234 patients with Klebsiella pneumoniae bloodstream infection admitted in the First Hospital of Qinhuangdao from January 2020 to December 2022 were retrospectively analyzed, including 202 cases admitted during January 2020 to June 2022 (model set), and 32 cases admitted during July to December 2022 (validation set). There were 64 cases died (fatal group) and 138 cases survived (survival group) within 28 d after admission in model set. Multivariate Logistic regression was used to analyze the risk factors of death in patients with Klebsiella pneumoniae bloodstream infection and a mortality prediction model was constructed. The constructed model was applied in validation set, and the consistency between predicted mortality and real mortality was analyzed. Results:Multivariate Logistic regression analysis showed that male sex ( OR=2.598, 95% CI 1.179-5.725, P=0.018), age≥65 years ( OR=4.420, 95% CI 2.029-9.627, P<0.001), admitted to intensive care unit (ICU) ( OR=10.299, 95% CI 4.752-22.321, P<0.001), and the empirical use of quinolones antibiotics ( OR=4.288, 95% CI 1.127-16.317, P=0.033) were independent risk factors for 28-day mortality in Klebsiella pneumoniae bloodstream infection patients. The regression equation for predicting the risk of death was -3.469+ male × 0.955+ age ≥ 65 years × 1.486+ admitted to ICU × 2.332+ empirical use of quinolone antibiotics × 1.456. The area under the ROC curve (AUC) for predicting death in the model set was 0.831, with sensitivity and specificity of 71.9% and 80.4%, respectively. The AUC for predicting death in the validation set was 0.881, with sensitivity and specificity of 91.7% and 75.0%, respectively. Conclusion:The constructed mortality prediction model in the study has good application value for the prognosis of patients with Klebsiella pneumoniae bloodstream infection.

Establishment and validation of prediction model of new-onset pelvic organ prolapse in early postpartum period / 中华妇产科杂志

Objective:To analyze the risk factors of pelvic organ prolapse (POP) and to establish the prediction model.Methods:A study was conducted on the prevalence of POP among 2 247 parturient women at 6 weeks postpartum in Peking University People′s Hospital from December 2018 to October 2019, and relevant influencing factors were analyzed to construct a prediction model of early postpartum POP using logsitic regression, which was validated internally. Data from November 2019 to December 2019 (403 parturient women) were collected for external validation of the prediction model. In addition, the obstetrical factors affecting the occurrence of early postpartum POP in 885 primiparas with vaginal delivery (from January 2019 to November 2019) were further discussed.Results:A total of 2 247 cases were included in the modeling group, and 403 cases were included in the external validation data set. POP accounted for 24.3% (545/2 247). Age, parity, body mass index before pregnancy, vaginal delivery and newborn birth weight were negative factors for early postpartum POP (all P<0.05). The nomogram was established based on the above factors, and internal and external verification indicated that the model had a good discrimination (C-index were 0.759 and 0.760, respectively). In addition, this study found that age and newborn birth weight were the main causes of early postpartum POP in primiparas with vaginal delivery ( P=0.044, P=0.004). Conclusions:The incidence of early postpartum POP is high. The prediction model of POP constructed in this study could be used to guide clinical practice to a certain extent and give corresponding treatment suggestions to pregnant women scientifically, so as to provide theoretical support for primary prevention.

Advantages and application progress of ultrasound-guided botulinum toxin injection / 中国介入影像与治疗学

Injections of botulinum toxin (BTX) are prescribed for various disorders, including spasticity, dystonia, pain and neurosecretory conditions, et al. Accurately targeting muscles for injection is one of the principle goals when performing BTX injection. Injections have been guided by anatomic landmarks, electromyography or electrical stimulation,as well as imaging techniques. Ultrasound imaging guidance has many benefits, including accuracy, low risk, no radiation and low cost. The advantages and application progress of ultrasound-guided BTX injection were reviewed in this article.

Ultrasound-guided suprascapular nerve block combined with glenohumeral steroid injection for treatment of adhesive shoulder capsulitis / 中国介入影像与治疗学

Objective: To observe the effect of ultrasound-guided suprascapular nerve block combined with steroid injection of glenohumeral joint for treatment of adhesive shoulder capsular inflammation. Methods: Totally 60 patients with adhesive shoulder capsulitis were randomly assigned into research group and control group (each n=30). Patients in research group received ultrasound-guided suprascapular nerve block and glenohumeral steroid injection on the basis of conventional rehabilitation management, while patients in control group received only conventional rehabilitation management. The visual analogue scale (VAS), Constant shoulder scale (CSS) level and shoulder range of motion (ROM) were evaluated before, 2 weeks and 4 weeks after treatment. Results: VAS and CSS of research group after 2 and 4 weeks were lower than those before treatment (all P<0.01), also lower than those of control group (all P<0.01). The ranges of shoulder flexion, abduction, internal rotation and external rotation in research group were higher than before treatment (all P<0.01) and higher than those in control group (all P<0.01, except for the range of rotation after 2 weeks of treatment). The recurrence rate in research group 3 and 6 months after treatment were lower than those in control group (both P<0.05). Conclusion: Ultrasound-guided suprascapular nerve block and glenohumeral steroid injection can improve the rehabilitation effect of patients with adhesive shoulder capsulitis and decrease recurrence.

Arthroscopic medial patellofemoral ligament reconstruction to treat recurrent patellar dislocation in adolescents / 中国医师杂志

Objective To investigate the procedure and efficacy of medial patellofemoral ligament (MPFL) reconstruction assisted with arthroscopy for recurrent patellar dislocation in adolescents.Methods From March 2012 to March 2017,15 adolescents suffered with recurrent patellar dislocation,who underwent MPFL reconstruction operation,were all adopted with autograft semitendinosus.The patellar side used the modified bone tunnels and the minimally invasive percutaneous grafts through bone tunnels,and then fixed in the femoral tunnel with absorbable interference screw.Follow-up included complications,subjective feeling,patellar apprehension test,recurrent dislocation.Knee function was evaluated by the Kujala score and Lysholm score.Results 15 patients were followed up for at least 12 months.All patients were satisfied with the treatment.No recurrent dislocation occurred.All the patients showed negative apprehension test.The Kujala score was improved from 53.2 ±5.27 (preoperative) to 93.0 ±4.13 (postoperative),with significant difference (t =21.84,P ＜ 0.05);the Lysholm score was improved from 54.5 ± 6.24 to 91.2 ±4.72,with significant difference (t =19.37,P ＜ 0.05).Conclusions MPFL reconstruction assisted with arthroscopy is an effective surgical procedure for adolescent patients wih recurrent patellar dislocation,which can improve the knee function with little trauma and complications.

Postpartum pelvic organ prolapse in women with cesarean section at different time during delivery / 中华妇产科杂志

Objective@#To study and analyze the incidence of pelvic organ prolapse (POP) in women who underwent cesarean section.@*Methods@#A cross-sectional study was conducted. The clinical data of the women who underwent regular antepartum examination and cesarean delivery in Peking University People′s Hospital from January 2019 to June 2019 were collected, including the cervical dilatation. The incidence of POP was analyzed.@*Results@#(1) A total of 345 women met the inclusion criteria, with cervical dilatation of 0-10 cm before cesarean section, the cervix was not dilatated in 58.6% (202/345) of the women, and the cervix was fully dilatated in 2.9% (10/345) of the women. There were 109 cases of POP stage Ⅰ or above, 13 cases of POP stage Ⅱ, and no stage Ⅲ or Ⅳ. The incidence of POP was 31.6% (109/345). (2) Statistical analysis showed that the cervical dilatation of the POP group was significantly larger than that of the non-prolapse group (median: 1.0 vs 0 cm;Z=-2.227, P=0.026). The risk of POP of vaginal trial-birth transfer to cesarean section was higher than that of elective cesarean section [42.4% (36/85) vs 28.1% (73/260), P=0.014]. However, the further analysis of Spearman correlation between cervical dilatation, elective cesarean section and POP, as well as the receiver operating characteristic curve showed that the correlation and prediction value were poor.@*Conclusion@#Elective cesarean section has a certain protective effect on postpartum prolapse, but the protective effect of vaginal trial-birth transfer to cesarean section on pelvic floor is weakened.

Contamination and pathogenicity analysis of listeria monocytogenes in restaurant in Heilongjiang Province / 中华预防医学杂志

Objective@#To study the contamination, serotype, pulsed field gel electrophoresis (PFGE) and drug resistance of listeria monocytogenes (L.monocytogenes) in the process of restaurant kitchens in Heilongjiang Province.@*Methods@#Seventeen typical restaurants were selected from three cities in Heilongjiang Province in 2016, and 590 kitchen samples were collected and tested according to the national standard method. The serotype, pulsed field gel electrophoresis (PFGE) and drug resistance of isolated strains were analyzed.@*Results@#L. monocytogenes was found in 104 of 590 of the samples analysed (17.63%). The isolates belong to six serotypes (1/2 a, 1/2 b, 1/2c, 3a, 3 b, 4 b) and self-condensing bacteria, and 57.38% (70 strains) of the strains belong to serotype 1/2b. Two highly pathogenic serotype 4b was detected for human listeria disease. The results of PFGE analysis show that the bacteria have cross-contamination in the environment, tools, equipment, food and personnel. The drug resistance results showed that 2 strains were resistant to tetracycline, 1 strain was resistant to erythromycin, 13 strains were intermediate to tetracycline, and 2 strains were resistant to tetracycline and erythromycin.@*Conclusion@#There is a certain degree of L. monocytogenes cross-contamination in the catering kitchen in Heilongjiang Province. And an important serotype 4b that can cause human Listeria disease was detected.

Interpretation for the group standards of the Isolation and Identification of Campylobacter jejuni and Campylobacter coli / 中华流行病学杂志

Foodborne Campylobacter is recognized as the leading causes of the bacterial diarrheal illness in both developing and developed countries. C. jejuni and C. coli caused 95% of the human campylobacterisosis. Bacteria culture has been recognized as the "Gold standard" for the diagnosis of the Campylobacter infection. The National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention coordinated the experienced researchers from China National Center for Food Safety Risk Assessment and other local Centers for Disease Control and Prevention to write up the standards for entitled Isolation and Identification of Campylobacter jejuni and Campylobacter coli (T/CPMA 006-2019). The standard is drafted with principles of emphasizing the scientific, normative, applicability and feasible nature. This group standard recommended the procedures and steps for the isolation and identification of C. jejuni and C.coli from variant samples. The standard aims to improve the capacity for Campylobacter identification in China.

Pelvic floor function of 5143 women in early postpartum stage and analysis on the effect factors / 中华妇产科杂志

Objective To describe the situation of early stage of pelvic floor function and investigate the effect factors in postpartum women.Methods A retrospective survey was conducted,and women who did regular examination and gave birth in Peking University People's Hospital and had an annual pelvic floor examination at 6-12 weeks after delivery from Sep.2012 to Dec.2017 were interviewed.General information and pelvic floor electrical physiological indexes were collected and analyzed.Results Totally 5 143 puerpera were included in the study.The normal strength of type Ⅰ muscle was 52.40％ (2 695/5 143) and the normal strength of type Ⅱ muscle was 52.69％ (2 710/5 143).The abnormal degree of fatigue of type Ⅰ muscle was 55.84％ (2 872/5 143) and the abnormal degree of fatigue of type Ⅱ muscle was 27.34％ (1 406 / 5 143).Pelvic floor dynamic pressure was (93 + 37) cmH2O (1 cmH2O=0.098 kPa).The incidence of stress urinary incontinence (SUI) was 23.49％ (1 208/5 143) after delivery and it was as high as 57.58％ (95/165) for women who had family history of pelvic floor dysfunction.Family history of pelvic floor dysfunction was the risk factor of the fatigue index of type Ⅰ and type Ⅱ muscle (P＜0.05).The incidence of SUI was associated with vaginal delivery and age (＞30 years old) and family history of pelvic floor dysfunction (P＜0.01).Conclusions Pelvic floor electrical physiological indicators are reduced in about half of women in early postpartum stage.Age (＞30 years old) and vaginal delivery and family history of pelvic floor dysfunction are the risk factors.

ApplicationofmultiGparameterMRIinthediagnosisofprostatecancer / 实用放射学杂志

Objective Toexploretheapplicationvalueofmulti-parametermagneticresonanceimaging(MP-MRI)inthediagnosis ofprostatecancer(PCa).Methods Theclinicaldataof50patientssuspectedasPCaandadmittedbetweenJanuary2017andJanuary 2018wereretrospectivelyanalyzed.Thepathologicalexaminationwasusedasthegoldstandard,andallpatientsunderwentMP-MRI. Theapplicationvalues(sensitivity,specificity,accuracy,positivepredictivevalue,andnegativepredictivevalue)ofMP-MRIwereevaluatedin thediagnosisofPCa.Results Amongsingleexaminationofeachsequence,thesensitivity,accuracyandnegativepredictivevalueof DWIwerehigher,whilethespecificityandpositivepredictivevalueofT2WIwerehigher.ThesensitivityofMP-MRIcombinationwas assameasthatofDWI,butthespecificity,accuracy,positivepredictivevalueandnegativepredictivevaluewerehigherthanthoseof anysingleexamination.PI-RADSscoresofthePCawerehigherthanthoseofthebenignprostatichyperplasiainT2WI,DWI,DCE-MRI and MP-MRIcombination(P＜0.05).TheADCvalueofthePCawaslowerthanthatofthebenignprostatichyperplasia(P＜0.05). Conclusion MP-MRIcombinationcanobtain moreeffectiveinformationforprostatediseases,andhashigherspecificity,accuracy, positivepredictivevalueandnegativepredictivevalueindiagnosingPCa.

Treatment of multiple cutaneous leiomyomas with surgery / 中华整形外科杂志

A 43-year-old female presented with multiple, painful, erythematous papules and nodules on the dorsal of the body over the past 17 years. The tumor was painful after cold stimulus and the significant symptoms improved for recent 3 years. Histopathological examinations confirmed the clinical diagnosis of cutaneous leiomyomas. A surgical procedure was one of the therapeutic options in the case of multiple and painful leiomyomas.

Molecular typing of Salmonella isolates from poultry production chains in four cities of Heilongjiang province / 中华流行病学杂志

Objective To study the PFGE type of Salmonella (S.) strains isolated from poultry production chains (hatching,breeding,slaughter,distribution and retail) of four cities in Heilongjiang province.Methods DNA collected from S.strains in 2012 was digested by Xba Ⅰ according to the standard PFGE protocol of US CDC.The PFGE patterns were then analyzed by BioNumerics software.Results The contamination of S.appeared most serious during the process of slaughtering (13.84％).PFGE was used to determine the genetic relationships between these isolates from poultry production chains,89 pulsotypes from 150 S.enteritidis isolates and 55 pulsotypes from 65 S.indiana isolates showed considerable diversity.The same pulsotypes ofS.enteritidis can be found between different food chains and cities.In contrast,no identical pulsotypes of S.indiana were found between different food chain and cities.In these four cities,the above said two kinds of S.were from different sources.The source of S.contamination in HLJ2 city had been traced back to the chain of poultry hatching.Conclusions The distribution of pulsetypes of the S.enteritidis and S.indiana isolates was from different regions and the dominant bands were also different between the chains of poultry production.Cross contamination existed in slaughterhouses and contamination can be traced back to the poultry hatching.

Molecular typing of Salmonella isolates from poultry production chains in four cities of Heilongjiang province / 中华流行病学杂志

Objective To study the PFGE type of Salmonella (S.) strains isolated from poultry production chains (hatching,breeding,slaughter,distribution and retail) of four cities in Heilongjiang province.Methods DNA collected from S.strains in 2012 was digested by Xba Ⅰ according to the standard PFGE protocol of US CDC.The PFGE patterns were then analyzed by BioNumerics software.Results The contamination of S.appeared most serious during the process of slaughtering (13.84％).PFGE was used to determine the genetic relationships between these isolates from poultry production chains,89 pulsotypes from 150 S.enteritidis isolates and 55 pulsotypes from 65 S.indiana isolates showed considerable diversity.The same pulsotypes ofS.enteritidis can be found between different food chains and cities.In contrast,no identical pulsotypes of S.indiana were found between different food chain and cities.In these four cities,the above said two kinds of S.were from different sources.The source of S.contamination in HLJ2 city had been traced back to the chain of poultry hatching.Conclusions The distribution of pulsetypes of the S.enteritidis and S.indiana isolates was from different regions and the dominant bands were also different between the chains of poultry production.Cross contamination existed in slaughterhouses and contamination can be traced back to the poultry hatching.

Phenotype and genotype of antimicrobial resistance on nasal Staphylococcus aureus isolates from healthy people / 中华流行病学杂志

Objective To investigate the antimicrobial susceptibility and molecular nature related to the resistance on macrolides from nasal Staphylococcus (S.) aureus isolates among healthy people.Methods A total of 100 S.aureus isolates collected from 2009 to 2011 were tested for antimicrobial susceptibility by E-test.Double disc test (D-test) was used to detect the inducible clindamycin resistance.All S.aureus isolates were characterized by spa typing.Macrolides resistance genes were detected and compared with isolates that were collected clinically or from the livestock.Results High resistance rates on erythromycin or clindamycin was noticed,with 52％ and 27％,respectively.Inducible clindamycin resistance was identified in 29 of the 100 (29％) isolates.In total,the 100 isolates were assigned to 35 spa types.The most common spa types were found to be t189,t571,t002,t796,t437,t034 and t701,that accounted for 51.0％ of all the isolates.erm (C) (57.7％) and erm (B) (34.6％) were found as the dominant genes in 52 S.aureus isolates from healthy people.On the other hand,erm (A) and erm (C) were identified in 95.0％ S.aureus isolates from patients and all the livestock,respectively.Conclusion erm (C) and erm (B) carrying S.aureus strains were circulating in healthy people and these genes were distributed in different S.aureus clones.

Research Progress in Chemical Constituents of Qi-Gui-Yin Compound / 世界科学技术-中医药现代化

Qi-Gui-Yin (QGY) compound, which composes of five herbal medicines of Astragalus membranaceus (Fisch.) Bge., Angelica sinensis (Oliv.) Diels, Lonicera japonica Thunb., Artemisia annua L., and Polygonum cuspidatum Sieb.et Zucc., which is able to treat antibiotic-resistant bacteria with antibiotics, and also to delay or even reverse the bacterial resistance producing. However, QGY compound is a complex mixture. And it is very difficult to elaborate its material foundation. Therefore, chemical constituents in five herbal medicines were summarized through searching of the related literatures. And then, 170 kinds of bioactive ingredients were classi-fied in order to provide evidences for the study on chemical constituent of QGY compound and identify its mate-rial foundation of delaying drug resistance .

Antimicrobial resistance profiles and genetic diversity of bovine staphylococcus aureus isolated in 5 provinces of China in 2013 / 中华预防医学杂志

<p><b>OBJECTIVE</b>To investigate antimicrobial resistance profiles and genetic diversity of staphylococcus aureus isolated from lactating cows of 5 provinces in China, 2013.</p><p><b>METHODS</b>A total of 680 samples were collected from 15 herds (12 farms, 3 artels) in 5 provinces of China in 2013, including swabs of extramammary sites (bovine teat skin and milking machine liners) and quarter milk samples from lactating cows diagnosed with subclinical mastitis. The antimicrobial resistance of the isolates were tested by broth microdilution method and the genotypes were determined by PFGE (pulsed-field gel electrophoresis) method.</p><p><b>RESULTS</b>A total of 111 isolates were isolated and identified as staphylococcus aureus. Resistance to penicillin (90.1% (100/111)), erythromycin (48.6% (54/111)), ciprofloxacin (36.9% (41/111)), clindamycin (27.9% (31/111)), gentamycin (18.9% (21/111)), chloramphenicol (9.0% (10/111)), tetracycline (7.2% (8/111)) of these strains were observed. All isolates were sensitive to oxacillin, vancomycin and selectrin. 92.8% (103/111) staphylococcus aureus isolates were resistant to at least one antimicrobial. 38.7% (43/111) strains were multi-drug resistant isolates. The resistance rate of isolates in artels (100% (48/48)) was higher than it in farms (87% (55/63)) and the difference was statistically significant (χ(2) = 4.80, P < 0.05). The multi-resistance rate of isolates in artels (54% (26/48)) was higher than it in farms (27% (17/63)) and the difference was also statistically significant (χ(2) = 8.48, P < 0.05). The 111 strains were clustered into 8 types, 6 out of which were consisted of 98% isolates (109/111), and were prevalent in 2 to 9 herds. Every herd had 1 to 4 types, and tend to be comprised by one major type. Most swab isolates were indistinguishable from isolates infecting the mammary gland. There were no relationship between antimicrobial resistance profiles and genotypes of these isolates.</p><p><b>CONCLUSION</b>The drug resistance of staphylococcus aureus isolates associated with lactating cows of 5 provinces in China is serious, especially the isolates collected from artels. A few specialized clones were responsible for most of the cases of bovine mastitis in a single herd and some clones might have a broad geographic distribution.</p>

Effect of high-sugar and high-fat diet on the morphology of rabbit knee articular cartilage / 中国组织工程研究

BACKGROUND: There is a positive correlation between the serum cholesterol and osteoarthrosis incidence. However, it is still unclear whether the high-sugar and high-fat diet participates in the process of articular cartilage degeneration leading to osteoarthrosis, and the participation mechanism is unknown. OBJECTIVE: To investigate the changes in knee cartilage morphology of New Zealand white rabbits fed with high-sugar and high-fat diet and to explore the effect of high-sugar and high-fat diet on articular cartilage degeneration. DESIGN, TIME AND SETTING: The randomized controlled experiment was performed in Animal Laboratory, Xiangya Medical College from January to September 2008. MATERIALS: Forty healthy adult male New Zealand white rabbits, weighing about 2 kg, were randomly divided into 4 groups with 10 in each group. METHODS: Male New Zealand white rabbits were divided into four groups: ①normal diet group(NG), ②high-sugar diet (mixture of 37% cane sugar and 63% normal fodder) group(SG), ③high-fat diet (mixture of 20% lard and 80% normal fodder) group(FG), ④high-sugar and high-fat diet (mixture of 37% cane sugar, 10% lard and 53% normal fodder) group(SFG), MAIN OUTCOME MEASURES: Fasting blood samples were extracted every 4 weeks. The levels of blood sugar, triglyceride, cholesterol and insulin were detected, and body mass was recorded. The femoral condyle cartilage were dyed with toluidine blue, observed with transmit electron microscope after general observation of rabbit knee joints at the 28th weeks. RESULTS: ①Blood sugar, triglyceride, total cholesterol in FG and SFG were obviously higher than that in NG, the level of blood insulin in FG and SFG increased first, and then decreased significantly (P 0.05). ② By light microscope and transmit electron microscope observation, the FG and SFG demonstrated that tide line disappeared, cartilage cells were ranged in disorder, collagen fibers fractured, cartilage cells and a change in shape, such as shrinkage. These changes of morphology were not observed in SG. CONCLUSION: Long-term high-fat diet or high-sugar and high-fat diet may induce or aggravate the disorder of articular cartilage, suggested that it may take part in the development of osteoarthritis.