Survey on Resident Behavior of Expired Drugs Disposal in Chongqing Families and Suggestions for Drug Recycling / 中国药房

OBJECTIVE:To provide reference to the construct recycling and disposal system for expired drugs of Chongqing families. METHODS:Questionnaire survey was conducted among 1 683 residents from 6 districts of Chongqing,involving whether or not to reserve and regularly check up the drugs in the home;how to dispose expired drugs;reasons for different disposal behavior;suggestions of drug recycling. Epidata 3.1 software was used for data entry and statistical analysis,and corresponding measures and suggestions were put forward. RESULTS:A total of 1 579 effective questionnaires were collected with effective recovery rate of 93.82%. 1 322 residents(83.72%)had the habit of storing drug;1 213 residents(91.75%)disposed expired drugs at random;109 interviewees had participated in expired drug recycling. Inquiries survey showed that 75 residents (68.81%)were not satisfied with the current way of expired drug recycling;90 residents(82.57%)suggested increasing recycling points;101 residents(92.66%)suggested setting recycling points near;93 residents(85.33%)suggested recovering expired drug to the doorstep;97 residents(88.99%)expected compensable drug recovery;1 062 residents who disposed expired drugs with the garbage had strong willingness to participate in drug recovery,and accepted different recycling methods well. CONCLUSIONS:Many residents have the habit of storing drugs,but they lack the consciousness of actively checking up the drugs. The residents deal with the expired drugs in the family casually,and lack the professional knowledge of expired drugs and environmental awareness. The residents who have been involved in recycling are not satisfied with the existing methods of recycling,and they lack of enthusiasm and initiative. The residents support professional expired drug recycling,but the actual behavior is affected by many factors. It is suggested to improve the relevant laws and regulations for the recycling and professional disposal of the expired drugs as soon as possible,construct government-leading,enterprise-participating and multi-lateral cooperative professional family expired drug recycling and disposal system.

Effects and mechanism of the circadian clock gene Per1 on the proliferation, apoptosis, cycle, and tumorigenicity in vivo of human oral squamous cell carcinoma / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To determine the regulatory effects of the circadian clock gene Per1 on cell cycle-related genes and its influence on the proliferation, apoptosis, cycle, and tumorigenicity in vivo of human oral squamous cell carcinoma SCC15 cells.</p><p><b>METHODS</b>Three groups of the short hairpin RNA (shRNA) of lentivirus recombinant plasmids were designed against the RNA of Per1 and then transfected to the SCC15 cells. The optimum interference group was screened through Western blot and quantitative real-time PCR (qRT-PCR) and assigned as the experimental group. The transfected lentivirus plasmid without an interference effect on any gene was set as the control group (Control-shRNA). Untreated SCC15 cells were set as the blank group. The mRNA expressions of cell cycle-related genes, namely, Per1, p53, Cyclin D1, Cyclin E, Cyclin A2, Cyclin B1, CDK1, CDK2, CDK4, CDK6, p16, p21, Wee1, cdc25, E2F, and Rbl1 in each group were detected through qRT-PCR. The cell proliferation, apoptosis, and cell cycle distribution in each group were evaluated through flow cytometry. The cells of the experimental group and the blank group were subcutaneously inoculated in nude mice to observe tumorigenesis.</p><p><b>RESULTS</b>Three groups of Per1-shRNA lentivirus plasmids were constructed successfully. Among the groups, the Per1-shRNA- I group exhibited the highest interference effect, as indicated by qRT-PCR and Western blot analysis. As such, this group was set as the experimental group. The mRNA expression levels of CyclinD1, CyclinE, CyclinB1, CDK1, and Wee1 gene in the Per1-shRNA-I group were significantly higher than those in the Control-shRNA group and the SCC15 group (P < 0.05). By contrast, the mRNA expression levels of p53, Cyclin A2, p16, p21, and cdc25 in the Per1-shRNA-I group were significantly lower than those in the Control-shRNA group and the SCC15 group (P < 0.05). The mRNA expression levels of each gene between the Control-sLRNA group and the SCC15 group did not significantly differ (P > 0.05). The mRNA expression levels of CDK2, CDK4, CDK6, E2F, and Rb1 did not significantly differed in the three groups (P > 0.05). The proliferation index of the Perl-shRNA-I group was significantly higher than those of the Control-shRNA group and the SCC15 group (P < 0.05). The apoptosis index of the Per1-shRNA-I group was significantly lower than those of the Control-shRNA group and the SCC15 group (P < 0.05). The number of S-phase cells in the Per1-shRNA-I group was significantly lower than those of S-phase cells in the Control-shRNA group and the SCC15 group (P < 0.05). The number of G2/M-phase cells in the Per1-shRNA-I group was significantly higher than those of G2/M-phase cells in the Control-shRNA group and the SCC15 group (P < 0.05). Conversely, the proliferation index, apoptotic index, and cell cycle distribution of the cells in the Control-shRNA group did not significantly differ from those of the SCC15 group (P > 0.05). The tumorigenic ability in vivo was significantly enhanced in the Per1-shRNA-I group (P < 0.05).</p><p><b>CONCLUSION</b>Per1 is an important tumor suppressor gene. Per1 can regulate a large number of downstream cell cycle-related genes. The alteration of its expression can affect cell cycle progression, proliferation, apoptosis imbalance, and tumorigenic ability in vivo. Further studies on Per1 may elucidate cancer development and provide novel effective molecular targets for cancer treatment.</p>

Exploration of ESP teaching in pharmaceutical management and administration major of higher vocational college / 中华医学教育探索杂志

With the development of the medicine industry and the expansion of global commu-nication and cooperation , more and more Chinese higher vocational college students who major in pharmaceutical management and administration are aware of the demands and are trying to master English for Specific Purpose (ESP) for their better future development. The necessity, course orienta-tion, curriculum design and teaching method for opening ESP course in pharmaceutical management and administration major of higher vocational college were studied in this paper.