RESUMO
Objective To explore the influence of catheterization for urinary tract infections and the importance of humanistic nursing. Methods Totally 248 patients who were admitted into our hospital from February 2013 to April 2014 were randomly divided into two groups. Patients in the control group (117 cases) were given spray fungicide and regular disinfection of urethra by nursing staff, and patients in the observation group (131 cases) were nursed by themselves or their family after been taught the way of spraying fungicide and cleaning. Ana-lyzed the psychological status, routine urine, urinary tract infections, nursing job satisfaction of the patients, and the relevant data were ana-lyzed by SPSS 17. 0 software. Results The third day after placing urinary catheter urethra, the relevant nursing can reduce the rate of urina-ry tract infections in patients. the difference of the two groups was statistically significant (P0. 05). Infection rate of male patients (29. 01%) is higher than female patients (10. 69%). The satisfaction rate of urethra care in the observation group was 87. 79%, and the satisfaction rate of other project were all higher than 92. 5%. The satisfaction rate of urinary tract nursing check in the control group was 79. 49%, and the satisfaction rate of nurse-patient communication was 83. 76%. It was found that the difference of communication between nurses and patients, service attitude and the urethra care were statistically significant. Conclusion It can reduce the rate of urinary tract infection after placing urinary catheter in pa-tients for 7 days and nursed by patients themselves with spraying fungicide and cleaning. Strengthen the humanistic nursing care can improve nursing satisfaction.
RESUMO
Objective To explore the cytotoxicity of the cytotoxic T lymphocyte (CTL) induced by SW480 sonicate sensitized dendritic cells (DC) on the colon cancer cell line SW480. Methods PBMC were separated from the HLA-A*0201 donor and DC were cultured with rhGM-CSF, rhIL-4 and rhTNF-α. The same donor's primary CTL were stimulated by DC loaded with SW480 sonicate. The cytotoxicity of CTL on SW480 (HLA-A*0201 positive) and K562 (HLA-A*0201 negative) was determined by the MTT method. Results The cytotoxicity of the CTL on SW480 was stronger than that on K562 (P ＜0.05). Conclusion The DC vaccine can stimulate specific CTL which can trigger cytotoxic activity on the target cells and this cytotoxicity is related to MHC restriction.