Preoperative medication timing analysis and effect on neovascular membrane vascular endothelial growth factor in patients with proliferative diabetic retinopathy assisted by Conbercept / 中国医师杂志

Objective To investigate the timing of administration of Conbercept in the treatment of proliferative diabetic retinopathy (PDR) before operation and its effect on neovascularization vascular endothelial growth factor (VEGF).Methods 90 patients (90 eyes) with PDR who underwent vitrectomy (PPV) were randomly divided into groups A,B and C,with 30 cases in each group.Group A received no intravitreal injection of Conbercept before operation,group B received intravitreal injection of Conbercept 3 days before operation,and group C received intravitreal injection of Conbercept 5 days before operation.The operation condition,visual acuity level,concentration of vascular endothelial growth factor in aqueous humor and positive expression of vascular growth factor in neovascularization membrane were compared in the three groups.Results There was no significant difference in the incidence of iatrogenic hiatus during operation,transient high intraocular pressure and hyphema after operation in the three groups (P ＞ 0.05).Compared with group A,group B and C had shorter the operation time,less electrocoagulation,lower LogMAR BCVA (best corrected visual acuity) three months after operation (P ≤ 0.05).There was no significant difference in the concentration of vascular endothelial growth factor between group B and group C before vitreous injection (P ＞ 0.05).The expression of VEGF in aqueous humor of group B and group C was lower than that of group A (P ≤ 0.05),and the total positive expression rate of VEGF in neovascularization membrane of group B and group C was 76.67％ and 73.33％ respectively,lower than that of group A (100.0％,P ≤0.05).The vitreous re-bleeding of group B and group C was 6.67％ and 10.0％,lower than that of group A (32.14％,P ≤ 0.05).While there was no significant difference between group B and group C (P ＞0.05).Conclusions Vitreous injection of Conbercept before PPV in PDR patients can reduce the concentration of VEGF in aqueous humor and the positive expression rate of VEGF in neovascularization membrane,significantly improve visual acuity and reduce the incidence of postoperative complications.The effect of vitreous injection 3 and 5 days before PPV is basically the same.

Effect of HAART on CD4+T cell and viral load in children with AIDS combined with HBV/HCV infection / 国际检验医学杂志

Objective To investigate the effect of high performance antiretroviral therapy on CD4+T cells, viral load and liver function in children with AIDS combined with HBV/HCV infection.Methods The simple HIV from April 2014to April 2015in our hospital infection in 88cases were divided into group A, 8cases of HIV patients with HBV infection was divided into B group, and 23cases of HIV patients with HCV infection was divided into C group, three groups were all treated with HAART, after about two years treatment time, before and after treatment of liver function, viral load and CD4+T cell changes between the three groups were compared.Results Before treatment, the total of three groups of children with abnormal liver function was 29cases (24.79％) , and the three groups were not statistically significant (P>0.05) .After treatment, the total of three groups of children with abnormal liver function were 82 (70.09％) , and the difference between the three groups was statistically significant (P<0.05) .Before treatment, there was no significant difference in the levels of AIT, AST and TBIL between the three groups (P>0.05) .After treatment, the levels of AIT, AST and TBIL in the three groups increased significantly (P<0.05) .The data between the three groups were not statistically significant (P>0.05) .Before treatment, there was no significant difference between the three groups of children with CD4+T cells and viral load (P>0.05);after treatment, the three groups of patients with CD4+T (P<0.05) cells increased, HIVRNA decreased significantly (P<0.05) , the three groups had no statistically significant difference between the two groups of patients (P>0.05) .Conclusion High-efficiency antiretroviral treatment in children with AIDS complicated with HBV/HCV infection, there are different differences in liver function of patients, after treatment can significantly increase the level of CD4+T cells, reduce viral load, AIDS combined with HBV/HCV infected children have different differences, the liver function should be paid close attention in clinical application.

Angle of acetabular cup in total hip replacement affects the safety of joint flexion and extension / 中国组织工程研究

BACKGROUND:Major complication after total hip replacement was instability in the form of dislocation. The probability of above complications has a great relationship with the mistakes of the angle of acetabular cup prosthesis. OBJECTIVE:To explore the influence of angle of acetabular cup in total hip replacement on the safety of joint flexion and extension. METHODS: A total of 60 patients, who underwent total hip replacement in the Wujin Hospital Affiliated to Jiangsu University from January 2012 to December 2014, were enroled in this study. Three dimensional directional monitoring device, CT scanning and image processing tools were used to preset the angle of the acetabular cup and to set abduction angle and anteversion angle. Test data were recorded and subjected to statistical analysis. RESULTS AND CONCLUSION:Acetabular cup was implanted under five different conditions: abduction angle 45°, anteversion angle 15°; abduction angle 60°, anteversion angle 15°; abduction angle 30°, anteversion angle 15°; abduction angle 45°, anteversion angle 5°; abduction angle 45°, anteversion angle 25°. According to statistics, in the patients with anteversion angle of 15°, when the acetabular cup was placed, the bigger the abduction angle, the bigger the range of abduction angle of the acetabular cup induced by pelvic tilt was. If the anteversion angle increased, the anteversion angle of the acetabular cup was reduced. With the increased pelvic tilt angle, the anteversion angle of the acetabular cup was big. Moreover, in patients with anteversion angle of 15°, the anteversion angle of the acetabular cup should keep consistent. When the abduction angle of the acetabular cup increased, the range of anteversion angle of the acetabular cup induced by pelvic tilt was diminished. These data showed that the abduction angle and anteversion angle of the acetabular cup have mutual restriction. When the acetabular cup was placed, big anteversion angle (15±10)° can wrap the femoral head, reduce the incidence of dislocation, and make flexion and extension safe.

Effect of low dosage of ciplatin on the shape and otoferlin in cochlea inner hair cells / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To establish the stable and efficient hearing damage model by using low dosage of cispla tin, and investigate the mechanism.@*METHOD@#C57 mice were divided into 7 groups (every group, n = 8), the first group was the control group, the others were separately intraperitoneally injected with different dosages of cispla tin for different time. We measured the auditory brainstem response (ABR) of the mice, and obtained the basal coil of organ Corti. We observed the shape of inner hair cells (IHC) by staining AgNO3 and marked otoferlin in the IHC by immunofluorescence,successively sliced by laser confocal microscopy. The RNA fragments were amplified by RT-PCR.@*RESULT@#After cisplatin administration for four days, the thresholds of the ABR improved in 1.5 mg/kg and 3.0 mg/kg group, and compared with the control group, the ABR thresholds improved in each group with ciplatin administration for seven days. With the same dosage, the ABR threshold of the 0.75 mg/kg x 7 d group was higher than 0.75 mg/kg x 4 d group, and there was no time-effect relationship existing in other groups with different dosage. The otoferlin was less expressed 3.0 mg/kg groups than the control group. However, the oto ferlin expressed in the 1.5 mg/kg groups were almost the same as the control group. The alteration of the IHC was observed most remarkablely in 3.0 mg/kg x 7 d group.@*CONCLUSION@#Low dosage of cisplatin can impair the hearing, and the expression of otoferlin may involve in the underlying mechanism.

Analysis of 18 cases which is the nasal sinuses adenoid cystic carcinoma / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the treatment and possible prognostic factor of nasal sinuses adenoid cystic carcinoma.@*METHOD@#Retrospectively analysed the records of 18 patients with complete clinical and pathological data,which including 4 patients given up treatment, 5 patients taken surgical treatment and 9 patients taken surgical treatment as well as radiotherapy and chemotherapy.@*RESULT@#Fifty percent of the patients got 2-year survival and 3 cases of death due to intracranial tumor invasion and 2 patients died of the disease distant metastasis.@*CONCLUSION@#If patients got Nasal sinuses adenoid cystic carcinoma, they should take comprehensive treatment based on surgery, in order to improve the survival rate. The prognosis depends on the tumor early detection and early treatment, the sooner the treatment, the better.

Sequeutial and concurrent chemo-radio therapy for stage Ⅲ non-small cell lung cancer / 国际肿瘤学杂志

With the development of medical technology,methods for treatment of lung cancer gradually diversified.Radiotherapy and chemotherapy have gradually become the main methods of treatment.Clinical study have found that simply adopting one kind of treatment,cannot achieve ideal control,with the result of low 5 year survival rate,and high recurrence rate and metastasis.Combining chemotherapy and radiotherapy,can achieve better effect than that by using single method of treatment,as non-small cell lung cancer (NSCLC) is particularly sensitive to the chemotherapy.Recent clinical findings indicate that treating late NSCLC patients with combined therapy methods could achieve significant clinical effect.

Curative effects of percutaneous vertebroplasty with poly(methyl methacrylate) versus conservative treatment on fresh osteoporotic thoracolumbar vertebral compression fractures / 中国组织工程研究

BACKGROUND:Percutaneous vertebroplasty for vertebral fractures can effectively relieve acute pain and has the advantages of smal trauma, good curative effect and less complications, but for patients with osteoporotic compression fractures, there were varying degrees of osteoporosis after surgery, which have a longer course of disease and cannot be easy to cure. So the effectiveness of percutaneous vertebroplasty cannot be ful y evaluated based on the pain relief. OBJECTIVE:To study the curative effect of percutaneous vertebroplasty for patients with fresh osteoporotic thoracolumbar vertebral compression fractures. METHODS:We selected 24 patients undergoing percutaneous vertebroplasty and 24 patients receiving conservative treatment at the same time who had fresh osteoporotic compression fractures as research objects;and compared pain degree, vertebral body height and the kyphosis Cobb angle, function activity of the lower lumbar before and after treatment, the quality of life and clinical incidence of complications within 6 months after treatment in the two groups. RESULTS AND CONCLUSION:The degree of pain, the vertebral body height, kyphosis Cobb angle, function activity of the lower lumbar were al improved in the two groups after treatment (P<0.05), and these indexes in the percutaneous vertebroplasty group were better than those in the conservative treatment group (P<0.05). The quality of life and incidence of complications within 6 months after treatment were improved better in the percutaneous vertebroplasty group than the conservative treatment group (both P<0.05). These results suggest that the percutaneous vertebroplasty for fresh osteoporotic thoracolumbar vertebral compression fractures can effectively reduce the pain of patients, improve vertebral deformity and activities of the lower lumbar, and has obvious role in promoting the postoperative quality of life of patients.