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Purpose@#RIOK1 has been proved to play an important role in cancer cell proliferation and migration in various types of cancers—such as colorectal and gastric cancers. However, the expression of RIOK1 in breast cancer (BC) and the relationship between RIOK1 expression and the development of BC are not well characterized. In this study, we assessed the expression of RIOK1 in BC and evaluated the mechanisms underlying its biological function in this disease context. @*Materials and Methods@#We used immunohistochemistry, western blot and quantitative real-time polymerase chain reaction to evaluate the expression of RIOK1 in BC patients. Then, knockdown or overexpression of RIOK1 were used to evaluate the effect on BC cells in vitro and in vivo. Finally, we predicted miR-204-5p could be a potential regulator of RIOK1. @*Results@#We found that the expression levels of RIOK1 were significantly higher in hormone receptor (HR)–negative BC patients and was associated with tumor grades (p=0.010) and p53 expression (p=0.008) and survival duration (p=0.011). Kaplan-Meier analysis suggested a tendency for the poor prognosis. In vitro, knockdown of RIOK1 could inhibit proliferation, invasion, and induced apoptosis in HR-negative BC cells and inhibited tumorigenesis in vivo, while overexpression of RIOK1 promoted HR-positive tumor progression. MiR-204-5p could regulate RIOK1 expression and be involved in BC progression. @*Conclusion@#These findings indicate that RIOK1 expression could be a biomarker of HR-negative BC, and it may serve as an effective prognostic indicator and promote BC progression.
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Objective:to construct and apply the bilingual teaching materials.Methods:teachers in Nantong university compiled Pathology,Direction of pathological experiment and test which were written in both Chinese and English as the in-class bilingual teaching materials according to the characteristics of Chinese students,meanwhile compiled Pathological formative exercises,Pathological listening and reading,Basic problems of modern clinical pathological research for students' practice and elective course materials or reading materials after class.Results:A series of 4 bilingual teaching materials were all published by science publishing house except Pathological listening and reading was used as in class teaching materials.These teaching materials played an important role in the bilingual pathological teaching in Nantong university.Conclusion:The construction and innovation of bilingual teaching materials are basic guarantees for bilingual teaching.
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Objective:To cultivate the students' learning ability and innovation spirit to better grasp the pathological knowledge,the Pathology Department of Nantong University carried out all-round research and practice of education reform in many links and many stages.Methods:To establish and perfect the teaching syllabus scientifically,combine class teaching with the second classroom activity as well as innovate examination mode and evaluation system.Results:The syllabus design not only fulfills the need of talent training,fits the actual level of the students,but also embodies the principle of the combining of imparting knowledge,fostering ability and raising quality.In addition,it also reflects the characteristics of pathology itself.Opening the second classroom activities of pathology is beneficial complementary to the course content.Various examination forms are used to have a comprehensive assessment of the students,creating an equal and fair competitive environment and competition,learning,exceeding atmosphere.Conclusion:The reform and innovation of pathological teaching of Nantong University can mobilize the students to learn with many sense organs all-directionally,match the cognition rules,promote the students' comprehension,apprehension and application about pathological knowledge/skills and embody the students' dominant position in teaching,which is warmly accepted by students.
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Objective To investigate the expressions and correlation between Survivin and PTEN proteins in astrocytoma.Methods The expressions of Survivin and PTEN proteins were examined by immunohistrochemistry in astrocytoma specimens from 65 patients with astrocytoma.Results The positive expression rate of Survivin in astrocytoma grade Ⅱ was significantly lower than that in grade Ⅲ and grade Ⅳ(all P