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Nucleotide excision repair was a complex biochemical process that involved in the repair of many kinds of DNA damage. Previous study suggested that xeroderma pigmentosum group C (XPC) gene played an important role in the process of DNA damage repair. This study aimed to explore the influence of XPC gene polymorphism on the prognosis of patients with colorectal cancer (CRC) who were treated with capecitabine-related adjuvant chemotherapy. METHODS: A total of 158 patients with CRC who received surgical resection and capecitabine-based adjuvant chemotherapy were included in this study consecutively. Baseline clinical characteristics of patients were collected and analyzed. Additionally, peripheral blood specimens of the patients were collected for polymorphism analysis of XPC gene and mRNA expression of XPC, respectively. The association analysis between XPC polymorphism and prognosis and mRNA expression was performed. Cox regression analysis was used for multivariate adjustment. RESULTS: Prognostic data in the 158 patients with CRC who received capecitabine-based adjuvant chemotherapy was collected retrospectively. The median follow-up duration of the patients was 5.0 years (range: 0.25-7.5 years). The median DFS and OS of the 158 patients with CRC was 4.5 years and 5.7 years, respectively. XPC polymorphism analysis suggested that rs2228001 was of clinical significance. The prevalence of rs2228001 polymorphism among CRC patients was: TT genotype 86 cases (54.4%), TG genotype 60 cases (38.0%) and GG genotype 12 cases (7.6%), resulting in a minor allele frequency of 0.27, which was in accordance with Hardy-Weinberg equilibrium (P=0.733). TG and GG genotypes were merged in the subsequent analysis. The prognostic results exhibited that the median DFS of patients with TT genotype and TG / GG genotype was 4.5 and 5.7 years, respectively (c
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0. 05). Conclusions Tinnitus is a common problem in the older population. With the aging of population, the problem will become more and more severe. More research is urgently needed on prevention and treatment of tinnitus in elderly people.
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Aim To observe the dysfunction of myocardial nuclear calcium transport in rat myocardial injury and effects of taurine on it . Methods The model of myocardial damage was induced by subcutaneous injection of isoproterenol (ISO,5 mg?kg-1?d-1). Myocardial nuclei were purified with Sucrose density centrifugation and identified zymologically. The activity of Ca2+_ATPase was measured zymologically and calcium uptake was assayed with 45Ca2+ isotope.Results Compared with the control, the Ca2+_ATPase activity of myocardial nuclear in ischemia group was decreased by 18.1%(P
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AIM: To study the effects of Liquestrazin on succinic dehydrogenase (SDH) and cytochrome oxidase (CCO) in the myocardial mitochondria of the ischemia-reperfusion rats and its mechanism. METHODS:Model of myocardial ischemia-reperfusion injury was produced by coronary artery ligation . The rats were devided into sham operation control (SC), ischemia-reperfusion (IR) and ischemia-reperfusion protected with Liqustrazin (IR+L) group . Activity of SDH,CCO,SOD and GSH?PX and contents of malondialdehyde (MDA),Cyt aa 3,Cyt c and phospholipid(PL) were observed respectively . RESULTS: As compared with ischemia-reperfusion group (IR), IR+L group showed significantly increased activity of SDH, CCO,SOD and GSH?PX (P