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Objective:To explore the clinical characteristics of hypertriglyceridemic pancreatitis and the correlation between triglyceride (TG) levels and the severity of severe acute pancreatitis (SAP).Methods:A retrospective analysis was conducted on the clinical data of 132 SAP patients admitted to Beijing Friendship Hospital of Capital Medical University from January 2020 to December 2021. They were divided into biliary acute pancreatitis (BAP) group, hypertriglyceridemic pancreatitis (HTGP) group, and alcohol-induced acute pancreatitis (AAP) group based on their etiology. The clinical data and laboratory test results of each group were compared, and the correlation between TG levels and the severity of SAP disease was analyzed.Results:The proportion of male patients, body mass index, length of hospital stay, ICU occupancy rate, C-reactive protein (CRP), TG, and total cholesterol (TC) levels in the HTGP group were significantly higher than those in the BAP and AAP groups. The age of onset, serum calcium (Ca), oxygenation index (PaO 2/FiO 2, P/F), and prothrombin time activity (PTA) in the HTGP group were significantly lower than those in the BAP and AAP groups, and the differences were statistically significant (all P<0.05). There was a negative correlation between TG levels and Ca, P/F, PTA in SAP patients ( r=-0.410, -0.341, -0.365, all P<0.01). Receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) of TG for early prediction of multiple organ dysfunction in SAP patients was 0.868, with a sensitivity of 87% and a specificity of 78%. Conclusions:Patients in the HTGP group have a lower onset age, with more males, obese patients, and critically ill patients. Laboratory indicators such as CRP, TG, and TC levels are higher, while levels of Ca, P/F, and PTA are lower; The serum TG level is correlated with the severity of SAP disease and can be used as one of the early indicators for predicting multiple organ dysfunction in SAP.
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Objective:To investigate the correlation between serum calcium level and patients with acute obstructive suppurative cholangitis (AOSC).Methods:The clinical data of 104 patients with AOSC treated in the emergency room of Beijing Friendship Hospital Affiliated to Capital Medical University from June 2019 to February 2020 were retrospectively selected, of which 53 patients with severe sepsis were included in group A, 51 patients with simple AOSC were included in group B, and 50 patients with non infectious severe diseases hospitalized in the same period were selected as control and included in group C. The levels of serum calcium, C-reactive protein (CRP), procalcitonin (PCT) and D-dimer (D-D) were compared in the three groups. Pearson analysis was used to analyze the correlation between serum calcium and CRP, PCT and D-D.Results:The levels of serum calcium in group A were significantly lower than those in group B and group C, and the levels of inflammatory related factors CRP, PCT and D-D were significantly higher than those in group B and group C ( P<0.01), with significant difference. In AOSC group, serum calcium was negatively correlated with CRP, PCT and D-D levels ( r=-0.550, -0.479, -0.431, P<0.05). Conclusions:Patients with AOSC are prone to hypocalcemia under infection, and the level of serum calcium is negatively correlated with infection inflammatory indexes CRP, PCT and D-D, indicating that the level of calcium is related to the severity of infection. Hypocalcemia should be found in time in clinical work, and the severity of the disease should be evaluated as soon as possible.
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OBJECTIVE@#To study the difference in age estimation based on quantitative analysis of DNA methylation by MassARRAY and pyrosequencing techniques.@*METHODS@#The methylation levels of 9 CpG sites from two independent whole blood sample sets (containing 65 and 62 samples) were detected using MassARRAY and pyrosequencing techniques. Z-score transformation was used to remove the batch effects of different techniques, and a linear regression model was used for age prediction.@*RESULTS@#For age prediction using the MassARRAY system, the 65 samples showed a mean absolute difference (MAD) of 2.49 years before Z-score transformation of the data and 2.44 years after the transformation, similar to the results in the 62 samples (MAD of 3.36 years before and 3.42 years after Z-score transformation). For data typed from pyrosequencing, the 65 samples showed a MAD of 4.20 years before and 2.76 years after data Z-score transformation, also similar to the results in the 62 samples (MAD of 3.92 years before and 3.63 years after data transformation).@*CONCLUSIONS@#Z-score transformation can effectively reduce the system batch effect between MassARRAY and pyrosequencing. Data from the MassARRAY system allows direct age estimation without further data processing, while the pyrosequencing data may increase the error in age estimation, which can be corrected by Z-score transformation of the data.
Assuntos
Ilhas de CpG/genética , Metilação de DNA , Sequenciamento de Nucleotídeos em Larga Escala , Modelos Lineares , Análise de Sequência de DNARESUMO
BacKground:The mechanism of diabetic gastrointestinaI dysmotiIity is stiII uncIear. More and more studies showed that gastrointestinaI smooth muscIe derived factors pIay an important roIe in gastrointestinaI dysmotiIity. RecentIy,the roIe of RhoA/ROCK signaIing pathway in diabetic compIications become a research hotspot. Aims:To investigate the possibIe roIe of RhoA/ROCK signaIing pathway in diabetic coIon dysmotiIity by examining the expressions of major signaIing moIecuIes in diabetic coIon muscIe. Methods:NormaI coIon tissue sampIes taken from patients undergoing radicaI surgery for coIonic cancer from Sept. 2012 to Dec. 2013 at the First AffiIiated HospitaI of Nanjing MedicaI University were coIIected. According to gIycated hemogIobin IeveI,the patients were divided into diabetes meIIitus( DM)group and controI group. The expressions of major signaIing moIecuIes in RhoA/ROCK1 signaIing pathway incIuding RhoA,ROCK1,MYPT1 and p-MYPT1 were determined by immunohistochemistry or Western bIotting. Results:Immunohistochemistry showed that expression of RhoA protein in DM group was significantIy Iower than that in controI group(P0. 05). Conclusions:The inhibition of RhoA/ROCK signaIing pathway in diabetic coIon muscIe may have some correIation with diabetic coIon dysmotiIity.
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Background:Myopathy due to smooth muscle cells( SMC)abnormalities is involved in the pathogenesis of diabetic gastroparesis(DGP),however,the relationship between myopathy and advanced glycation end products(AGEs)is not fully clarified. Aims:To investigate the ultrastructural changes of gastric SMC in patients with diabetes mellitus( DM)and the relationship between expressions of contractile proteins and AGEs. Methods:Full-thickness gastric specimens from 30 gastric neoplasm patients undergoing gastrectomy from July 2012 to December 2012 at the First Affiliated Hospital with Nanjing Medical University were collected. Of them 15 patients had DM( DM group)and the other 15 patients without ( control group). Ultrastructure of SMC was observed by transmission electron microscopy. Expressions of three contractile proteins[ myosin heavy chains( MHC),α-actin and calponin]and Nε-carboxymethyllysine( CML),the key component of AGEs in gastric muscular layer were determined by Western blotting,and expressions of MHC,α-actin and calponin mRNA were determined by real-time PCR. Correlations of mRNA expressions of three contractile proteins with protein expression of CML were analyzed by Pearson correlation coefficient. Results:In DM group,significant ultrastructural changes were found in gastric SMC,including disruption of gap junction,swelling of mitochondria,occurring of lipofuscin in cytoplasm,increase in cell membrane alveolae,and thickening of basal lamina. Protein and mRNA expressions of muscular MHC,α-actin and calponin were significantly lower in DM group than in control group(P all ﹤0. 01),while protein expression of CML was significantly higher in DM group(P ﹤0. 01). In gastric muscular layer of DM group, negative correlations were found between expressions of MHC,calponin mRNA and CML protein(r= -0. 59,P=0. 02;r= -0. 63,P=0. 01),but no correlation was seen between α-actin mRNA and CML protein(r= -0. 49,P=0. 06). Conclusions:Disruption of SMC ultrastructure,decrease in contractile proteins and increase in AGEs are existed in gastric muscular layer of DM patients,and there is a negative correlation between contractile proteins and AGEs. These changes may cause impaired contractility of SMC,and subsequently lead to gastric motility disorders in diabetic patients.