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Objective To investigate the effect of anti-human immunoglobulin M (IgM) on proliferation,apoptosis,cell cycle and tumor formation in human nasopharyngeal carcinoma HNE-1 cell line in vitro and in vivo.Methods After treatment with anti-human IgM antibody,proliferation of HNE-1 cells was observed by cell proliferation inhibition assay,apoptosis and cell cycle of HNE-1 cells were detected by flow cytometry,and apoptotic cells were detected by TUNEL staining.Nude mouse models were constructed,and were injected intraperitoneally with anti-human IgM antibodies (once every 3 days).The growth of transplanted tumor was observed once every 4 days.After the fifth injection,the expression levels of IgM and gp96 protein in transplanted tumor were observed by immunohistochemical method (streptavidin-peroxidase conjugated method,SP).Results MTS assay showed that anti-human IgM antibody can significantly inhibit the proliferation of HNE-1 cells in concentration-and time-dependent manner (P＜0.05).Flow cytometry showed that the anti-human IgM antibody promoted a significant decrease in percentage of cells in G1 phase,a significant increase in percentage of cells in S phase,and a significant increase in apoptotic rate of HNE-1 cells (P＜0.05).TUNEL staining showed that the anti-human IgM antibody promoted apoptosis of HNE-1 cells (P＜0.01).Transplantation tumor experiment showed that anti-human IgM antibody can significantly inhibit the volume and weight of transplanted tumor (P＜0.05).The immunohistochemistry showed that the expression levels of IgM and gp96 proteins in mouse transplanted tumors after intraperitoneal injection with anti-human IgM antibodies were significantly lower than those of the control group (P＜0.05).Conclusion The anti-human IgM anti-body could effectively inhibit the proliferation of HNE-1 cells,promote apoptosis,and arrest cell cycle.Anti-human IgM antibody could also inhibit the growth of transplanted tumor in nude mouse,which might be related to inhibition of the expressions of IgM and gp96 proteins.
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Objective To observe the clinical effect of 0.9％ sodium nasal irrigation to adjuvant treatment of allergic rhinitis with postnasal drip symptoms.Methods Fifty-eight patients of allergic rhinitis with postnasal drip symptoms were divided into two groups by random number table method,29 cases in each.The treatment group was treated on the basis by antihistamine drugs in the specification,nasal steroid and nasal decongestants,given 0.9％ sodium to nasal irrigation before nasal steroid;the control group was not given 0.9％ sodium to nasal irrigation before nasal steroid,treatment for 6 weeks,improvement of allergic rhinitis symptoms and postnasal drip symptoms were observed in two groups.Results The clinical efficacy of allergic rhinitis:the total effective rate in treatment group was higher than that in control group [96.6％ (28/29) vs.72.4％ (21/29)],which reached statistical significance (P ＜ 0.05).The clinical efficacy of postnasal drip symptoms:the effective rate in treatment group was higher than that in control group [89.7％ (26/29) vs.58.6％(17/29)],which reached statistical significance(P＜ 0.05).Conclusions 0.9％ sodium nasal irrigation can improve the nasal symptoms of allergic rhinitis,reduce postnasal drip symptoms at the same time.It is a safe and effective treatment method.