Evaluation of morphological and structure parameters of tricuspid in patients with functional tricuspid regurgitation using real-time three-dimensional transthoracic echocardiography / 中华超声影像学杂志

Objective:To explore the morphological parameters of tricuspid in patients with moderate or above functional tricuspid regurgitation (FTR ) by real-time three-dimensional (3D) transthoracic echocardiography.Methods:A total of 31 moderate or above FTR(FTR group) and 36 healthy adults(control group) were included in the study from May to July 2020 in the First Affiliated Hospital of Air Force Medical University (Xijing Hospital). Two-dimensional (2D) and 3D echoardiography were performed on both FTR group and control group. The 2D parameters included tricuspid regurgitation (TR), transverse diameter of right atrium/ ventricle, transverse diameter of tricuspid annulus(TVD), left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS). The tricuspid 3D parameters were analyzed by TomTec software, including annular parameters[annular anterior-posterior diameter (AP), annular left-right diameter (AL-PM), sphericity index (SI=AP/AL-PM), non-planar angle (NPA), annulus circumference (AC), annulus area (AA), annulus height (AH)], leaflet parameters[tenting volume(TV), tenting area(TA), tenting heigh(TH), commissural diameter(CD)], and dynamic parameters[annular displacement max(ADmax), annulus area fraction(AAF)].Results:Compared with the control group, AP, AL-PM, SI, AC, AA, TV, TA parameters of FTR group were larger, ADmax was smaller (all P<0.05). Multiple linear regression model was used to evaluate the effects of RA, RV, AP, AL-PM, SI, TV, TA on FTR, and the results showed that FTR was associated with TV and TA( t=4.253, -2.14; all P<0.05). Conclusions:RT-3DE can be used to assess the morphological parameters of tricuspid valve with moderate or above FTR patients effectively. TV and TA are the two most important factors for moderate or above FTR.

Correlation of the human papillomavirus infection status and genotyping with the result of liquid-based thin-layer cytology of cervix in the female physical examiners in Xi'an / 肿瘤研究与临床

Objective@#To investigate the correlation of human papillomavirus (HPV) infection status and genotyping characteristics with the result of liquid-based thin-layer cytology (TCT) of the cervix in the female physical examiners in Xi'an.@*Methods@#The data of 5 163 female physical examiners who underwent HPV and TCT screening simultaneously in the First Affiliated Hospital of Xi'an Jiaotong University From June 2017 to June 2018 were collected to analyze HPV infection, genotyping and their relationship with TCT.@*Results@#Among the 5 163 examiners, 527 (10.21%) were HPV-positive, and the infection rate in the examiners over 60 years old was the highest [12.26% (57/465)]. Among HPV-positive examiners, the infection rate of single genotype was higher than that of multiple genotypes infection [75.90% (400/527) vs. 24.10% (127/527), P < 0.01]. The top four genotypes were type 52, 58, 53 and 16 accounting for 17.21% (121/703), 12.09% (85/703), 11.52% (81/703) and 10.67% (75/703), respectively, of the total frequency of HPV infection. Of the 5 163 examiners, 68 (1.32%) examiners were positive for TCT screening. There was no significant difference in the positive rate of TCT between HPV multiple genotypes-infected examiners and single genotype-infected examiners [11.02% (14/127) vs. 9.00% (36/400), χ2 = 0.460, P > 0.05]. Among 50 examiners with positive results of HPV and TCT tests, the top four genotypes were type 58, 52, 53 and 16, and they were in consistence with the overall HPV infection genotyping.@*Conclusions@#The HPV infection in the female physical examiners in Xi'an is dominated by the single genotype infection, and the main genotypes of HPV infection are type 52, 58, 53 and 16. The combined detection of HPV genotyping and TCT can improve the effectiveness of cervical lesions screening.

Optimization of Prokaryotic Expression Conditions of Human β2-microglobulin in E. Coli and Its Purification / 生物医学工程学杂志

To obtain recombinant human β2-microglobulin (rhβ2M) with properties of good solubility and high purity from E. coli, prokaryotic expression conditions were optimized and protein purification was performed in this study. After testing the effect of different IPTG concentrations, temperatures and induction times on the production of rhβ2M, the optimum expression conditions were determined, i. e. joining IPTG to final concentration being 0.8 mmol/L and inducing time 6 h and at temperature of 25 degrees C. Under the optimum induction conditions, the ratio of soluble rhβ2M to soluble bacterial protein was 63.7%. After purified by Ni Sepharose 6 Fast Flow, the purity of rhβ2M achieved a greater value of 95%. Western blot analysis revealed that rhβ2M possessed the antigen property that specifically interacted with anti-β2M antibody.

Echocardiographic diagnosis of congenital mitral valve malformation / 中华超声影像学杂志

Objective To investigate the clinical value of the echocardiography in the diagnosis of congenital mitral valve malformation and summarize its occurrence and type associated with other cardiac malformations.Methods The echocardiographic image data of 101 patients with congenital mitral valve malformation were retrospectively analyzed,the characteristics of different types were summarized and compared with operation results,CT,cardiac catheterization,transesophageal echocardiography,as well as the data of echocardiographic follow-up.Results Among 101 patients with echocardiographic examination showed mitral valve congenital malformation,63 cases were isolated valve set malformation (62.4％),and multiple valve set malformation were found in 38 cases (32.7％).In another side,39 cases were of single mitral valve malformation (38.6％),while 62 patients combined with other type cardiac malformations (61.4％).No significant difference of dynamic change was shown between patients with solitary and multiple malformation of mitral valve (P ＞0.05).Conclusions Echocardiography can display congenital mitral valve malformation in a fast,robust,real-time way.It has an important clinical value in the diagnosis of congenital mitral valve malformation.

Measurement and correlation analysis of plasma VEGF level in the patients of hyperthyroidism / 生物医学工程学杂志

Vascular endothelial growth factor (VEGF) is a glycoprotein that promotes endothelial regeneration, stimulates formation of collateral blood vessels and increases vascular permeability. The purpose of this study was to measure the peripheral blood plasma level of VEGF and FT3, FT4, TSH and to analyze the correlation of the level of VEGF and TSH, FT3, FT4, age and gender in the patients of hyperthyroidism. The relationship between hyperthyroidism and VEGF was investigated as well. The plasma level of VEGF in 45 hyperthyroidism patients and 27 healthy persons were measured by enzyme-linked immunosorbent assay (ELISA), while plasma FT3, FT4, TSH were detected by chemiluminescence. The result showed that the plasma level of VEGF in hyperthyroidism patients [(92.53 +/- 62.38) pg/mL] was significantly lower than that in the control group [(158.28 +/- 77.15) pg/mL] (P < 0.01). The plasma level of VEGF correlated with age, and that of those over 40Y was significantly higher than that of 40Y or younger (P < 0.05) in healthy group. Whereas there was no correlation among VEGF, TSH, FT3, FT4, age and gender in hyperthyroidism patients (P > 0.05). These results suggested that the peripheral blood plasma level of VEGF in hyperthyroidism patients was significantly lower than that in the control group. Further experimental investigations are needed to estimate the relationship between VEGF and hyperthyroidism.

Advances in study of vascular endothelial growth factor and thyroid disease / 生物医学工程学杂志

The basic function of vascular endothelial growth factor (VEGF) is to promote vascular growth. Recent studies show that VEGF plays a vital role in various thyroid diseases through prompting angiogenesis. But the function of VEGF and specific change condition in different thyroid disease still needs further explorations. This review mainly focuses on the molecular characteristics and clinical application of VEGF.