Effect of Zibai Gelatin for Cervical Cancer SiHa Cells / 中国中医药信息杂志

Objective To investigate the immunological reaction mechanism of medicated serum in Zibai Gelatin for SiHa cells of the cervical cancer infected by high risk human papilloma virus (HPV). Methods Through immunohistochemical comparison and the cell culture, and after medicated serum was administrated to SiHa cells of cervical cancer for 24 h, 48 h, and 72 h, optical densities of IL-6, IL-10, CD83 and TNF-αin the same time but different concentrations and different times but the same concentration were observed. Relationships of dose-effect and time-effect between expressions of IL-6, IL-10, CD83 and TNF-α and medicine action were analyzed by calculating average optical density. Results With the increase of medicine concentration and administration time of Zibai Gelatin, the expressions of IL-6 and TNF-αgradually decreased, while the expressions of CD83 and IL-10 gradually increased (P<0.05). Conclusion Zibai Gelatin with medicated serum can inhibit local inflammatory reaction by improving local immunologic function of cervix, which is beneficial to reduce cervix high-risk HPV.

Effect of ferulic acid on proliferation and mechanism in human breast cancer cells / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate phytoestrogenic effects of ferulic acid in ER-positive T47D and ER-negative MDA-MB231 cells in culture.</p><p><b>METHOD</b>T47D and MDA-MB231 human breast cancer cells were treated with ferulic acid and examined cell proliferation by means of MTT assay. Cell cycle distribution, ERalpha and ERbeta expression were treated by flow cytometer. The pS2 mRNA expressions were detected by real-time fluorescence quantitative PCR.</p><p><b>RESULT</b>The proliferations were enhanced significantly by treatment with ferulic acid on T47D cells and the proliferation effects were inhibited by adding Faslodex (1 x 10(-8) mol x L(-1)). However, there was no significant difference on the proliferation in MDA-MB-231 cells compared with solvent control group by both treatment with ferulic acid and co-treatment with Faslodex (1 x 10(-8) mol x L(-1)). Ferulic acid stimulated the amount of T47D cells in phase S and proliferation index increased significantly. The effects were inhibited by treatment with Faslodex (1 x 10(-8) mol x L(-1)), and the amount of cells in phase S and proliferation index decreased, the amount of cells in G0/G1 phase increased, cell cycle of T47D was arrested in G0/G1 phase. Ferulic acid up-regulated pS2 mRNA expressions and increased the level of ERalpha protein expression in T47D cells. Ferulic acid did not show remarkable effect to the level of ERbeta protein expression in T47D cells.</p><p><b>CONCLUSION</b>Ferulic acid possessed phytoestrogenic effect by up-regulating pS2 gene expression and the receptor subtype of ERalpha.</p>

Diagnostic Methods of Traditional Iranian Medicine / 国际中医中药杂志

"Traditional Iranian Medicine" (TIM) are different in basic theories, diagnosis and treatment methods. A common diagnostic method of them is "Pulse Diagnosis", but is used with different purposes and techniques. This article describes the similarities of pulse diagnosis raditional Iranian Medicine.

Study on phytoestrogenic-like effects of four kinds of Chinese medicine including Radix Rehmanniae Preparata, Radix Paeoniae Alba, Radix Angelicae Sinensis, Rhizoma Chuanxiong / 中国中药杂志

<p><b>OBJECTIVE</b>To explore the phytoestrogenic-like effects of four kinds of Chinese medicine including Radix rehmanniae preparata, Radix Paeoniae Alba, Radix Angelicae Sinensis, Rhizoma Chuanxiong.</p><p><b>METHOD</b>Sixty immature female SD rats weighting (70 +/- 5) g were randomly divided into six groups: normal control group, positive control group and 4 Chinese medicine groups. The rats in different groups were treated for 4 days. On the fifth day, animals were sacrificed and uteri were separated solely and weighed. The blood was collected, and serum was separated. The effect of the pharmacological serum on proliferation assay with human breast cancer cell line (MCF7) by MTT method. Cell-cycle analyses were carried out with propidium iodide staining by flow cytometer. The expressions of subtypes-estrogen receptor-alpha (ERalpha) were detected by flow cytometry.</p><p><b>RESULT</b>Radix Rehmanniae Preparata, Radix Paeoniae Alba and Radix Angelicae Sinensis could increase the immature rat's uterus wet weight and the ratio of uterus to body weight (P < 0.05). The pharmacological serum of the four kinds of Chinese medicine stimulated proliferation of MCF7 cell respectively compared with normal control group (P < 0.01). The cell cycle was impulsed from G1 to S, DNA synthesizing was inhanced, and PI was also increased. The pharmacological serum of Radix Angelicae Sinensis and Rhizoma Chuanxiong could increase the expressions of (ERalpha) (P < 0.05).</p><p><b>CONCLUSION</b>Radix Rehmanniae Preparata, Radix Paeoniae Alba and Radix Angelicae sinensis have phytoestrogenic effects. But the data werent consistent with in vitro and in vivo assay of Rhizoma Chuanxiong.</p>

Regulation of genistein on the levels of ER?, ER? mRNA in uterine endometrial cancer cells / 北京大学学报(医学版)

Objective: To explore the mechanism of phytoestrogen (genistein) on uterine endometrial cancer cells and provide the theory foundation for using phytoestrogen in clinical practice. Methods: Uterine endometrial cancer cell lines (ishikawa) were cultured in vitro at different genistein concentration, and the expressions of the two estrogen subtypes of endometrial cancer cells were observed by real-time PCR. And two treatment groups, estradiol and progestin, were used as positive controls. Results:(1) Low concentration of genistein could enhance the expression level of ER? mRNA of endometrial cancer cells, which was obviously weaker than that of the estradiol group. On the other hand, low concentration of genistein could decrease the expression level of ER? mRNA of endometrial cancer cells, however estradiol had hardly any effect on this .(2) High concentration of genistein could decline the expression levels of ER? and ER? mRNA of endometrial cancer cells, whose effect was weaker than that of progestin. Conclusion: The expression levels of ER? mRNA and ER? mRNA of endometrial cancer cells vary with genistein concentrations. The experimental results suggest that genistein may act as estrogen receptor regulator.

EFFECT OF OVARIECTOMY ON THE EXPRESSION OF ESTROGEN RECEPTER SUBTYPE IN THE ADRENAL GLANDS OF THE RATS / 解剖学报

Objective The mechanism of the influence on adrenal glands of the ovariectomy rats was studied by observing the expression of estrogen receptor subtype in adrenal glands of the rats. Methods Twenty adult female Wistar rats were divided into 2 groups:the sham-operated group(10 rats) and the model group(10 rats).After 6 weeks all rats were killed and the adrenal glands were excised,and the tissues in each group were studied with the method of immunohistochemistry.The level of estrogen(E2) and follicle stimulating hormone(FSH) in blood serum was measured by radiation immunohistology. Results 1.Staining for ER? and ER? were the densest in zona fasciculats and zona reticularis with lesser staining in the zona glomerulosa.ER? expressed weaker than ER?.2.ER? expressed in the sham-operated groups and less intensity than in the model groups,it had statistically significant.3.The level of FSH in the serum of the ovariectomy rats was increasing and the E2 was decreasing,there was great difference between the sham-operated groups and the model group.Conclusion The expression of either ER? or ER? on the rat adrenal gland was obviously influenced by ovariectomy,especially the expression of ER?.Ovariectomy could decrease E2 in serum and increase FSH which excretes from pituitary through feedback.