RESUMO
A simple, sensitive, selective, and reproducible liquid chromatography-tandem mass spectrometric method was developed for the simultaneous determination of repaglinide and metformin in human plasma using d5-repaglinide and d6-metformin as internal standards (ISs). After a simple protein precipitation using acetonitrile as the precipitation solvent, both analytes and ISs were separated on a Venusil ASB C 18 (150 mm x 4.6 mm, 5 microm) via gradient elution using acetonitrile--10 mmol x L(-1) ammonium acetate as the mobile phase. A chromatographic total run time of 7.5 min was achieved. Mass spectrometric detection was conducted with atmospheric pressure chemical ionization under positive-ion and multiple-reaction monitoring modes. The method was linear over the 0.2 to 60.0 ng x mL(-1) concentration range for repaglinide and over the 4 to 1 000 ng x mL(-1) range for metformin. For both analytes, the intra- and inter-accuracies and precisions were within the +/- 15% acceptable limit across all concentrations. The validated method was successfully applied to a clinical bioequivalence study.
RESUMO
According to the characteristics of four basic P morphologies, combining the wavelet transform and the amplitude and slope of transformed P wave, a new P-wave detecting method based on "wavelet-amplitude-slope" algorithm is presented: First search out all modulus maximum pairs to satisfy the threshold after wavelet transform, and then applying the amplitude and slope criterion exclude the interferes and detect the P peak and its shape, last determine the onset and end of P wave respectively which should be separately calculated for single-peak and double-peak P wave (or biphasic P wave). The approach is applied in experiments of data from MIT/BIH database and randomly collected data of clinical ECG. The experimental statistical results shows that the correct detecting rate is as high as 96% compared to manual annotation.