Comparison of effects of different enteral nutritional agents on nutritional status and intestinal mucosal barrier in patients with septic shock / 中国中西医结合急救杂志

Objective To investigate the effects of different early enteral nutrient (EN) emulsions of TPF-T and TP on nutritional status and intestinal mucosal barrier in patients with septic shock. Methods From May 2017 to May 2018, 112 patients with septic shock were continuously enrolled in the Department of Intensive Care Unit of the First People's Hospital of Taizhou, and they were randomly divided into a TPF-T group and TP group, each group with 56 cases. After admission, the patients in both groups were all treated according to the 2016 Saving Sepsis Campaign (SSC) Guidelines for septic shock. Both groups were supported with EN, TPT-T group was given TPF-T EN emulsion rich in fish oil, while TP group was supported with standard TP EN emulsion, and the therapeutic course was consecutive 7 days in both groups. The differences in nutritional status, inflammatory response, immune function, intestinal mucosal barrier, gastrointestinal symptoms and prognosis were compared between the two groups. Results After EN, the nutrition indicators such as albumin (Alb), prealbumin (PA), transferrin (TRF) and immune indexes (IgA, IgG), human leukocyte DR antigens (HLA-DR) and D-lactic acid were increased in both groups, reaching the peaks on the 7th day after EN application, Alb, PA, TRF, IgA, IgG, HLA-DR in the TPF-T group were significantly higher than those in the TP group [Alb (g/L): 34.43±5.81 vs. 33.59±5.34, PA (mg/L): 269.83±47.56 vs. 252.67±41.92, TRF (g/L): 3.43±0.64 vs. 3.32±0.81, IgA (mg/L): 159.45±34.56 vs. 143.31±33.81, IgG (mg/L): 4 947.68±871.66 vs. 4 583.75±841.54, HLA-DR: (68.22±9.11)% vs. (62.21±9.69)%], and after EN, the D-lactic acid in the TPF-T group was significantly lower than that in the TP group (mg/L: 30.42±6.79 vs. 33.34±7.31). The inflammatory indicators of tumor necrosis factor-α (TNF-α), C-reactive protein (CRP), procalcitonin (PCT), endotoxin and diamine oxidase (DAO) were all gradually reduced in two groups, reached the lowest levels on the 7th day after EN application, and all the above-mentioned indicators in the TPF-T group were significantly lower than those in the TP group [TNF-α (ng/L):95.43±20.69 vs. 109.59±23.45, CRP (mg/L): 21.33±16.35 vs. 32.36±17.83, PCT (μg/L): 1.24±1.21 vs. 4.18±1.32, endotoxin (U/L): 10.32±2.31 vs. 11.54±2.69, DAO (g/L): 19.45±8.49 vs. 25.47±9.41]. The incidences of gastric retention, diarrhea and paralysis of lower digestive tract in TPF-T group were significantly lower than those in TP group [gastric retention: 14.29% (8/56) vs. 32.14% (18/56), diarrhea: 12.50% (7/56) vs. 35.71% (20/56), paralysis of lower digestive tract: 7.14% (4/56) vs. 23.21% (13/56)], the length of hospital stay was significantly shorter in the TPF-T group than that in the TP group (days: 18.77±5.08 vs. 21.71±6.67, P < 0.05); however, there was no significant difference in mortality between the two groups [14.29% (8/56) vs. 21.43% (12/56), P > 0.05]. Conclusion TPF-T could more effectively maintain nutritional status, reduce inflammatory reaction, improve immunity, protect intestinal mucosal barrier function, and has fewer adverse reactions, which was helpful to improve the prognosis of septic shock patients.

Effect of amikacin and prulifloxacin alternate application in the treatment of lung infections in ICU resistant acinetobacter bauman / 中国生化药物杂志

Objective To investigate the effect of Amikacin and prulifloxacin alternate application in the treatment of Lung infections in ICU resistant Acinetobacter Bauman.Methods 82 cases of Lung infections in ICU resistant Acinetobacter Bauman from August 2014 to August 2016 in our hospital were selected and randomLy divided into two groups, 41 cases in control group were given routine treatment, 41 cases in the experimental group were treated with Amikacin and prulifloxacin alternate application, and patients were treated continuous for two weeks.Levels of serum CRP, PCT, WBC, N%, SCR, BUN, AST, ALT, scores of APACHE II and CPIS and clinical efficacy were observed pre-and post-treatment.Results After two weeks, levels of serum CRP, PCT, WBC and N% in experimental group were significantly lower than control group, scores of APACHE II and CPIS were significantly lower than the control group, the total clinical efficiency was higher than the control group(P<0.05), and the levels of serum SCR, BUN, ALT, AST were lower, but had no statistically difference.Conclusion Amikacin and prulifloxacin alternate application in the treatment of Lung infections in ICU resistant Acinetobacter Bauman can reduce the patient's inflammatory reaction and the degree of infection.

The novel species and genus discovered and nominated from the human oral cavity in 2009--2012 / 华西口腔医学杂志

Bacterium is dominant microflora population in human oral cavity, and the novel species and novel genus were discovered and named one after another. This article reviewed the major biological characteristics of 5 novel genus and 16 novel species isolated from the human oral cavity from 2009 to 2012.

Scanning electron microscope observation of morphological influence P-aminobenzoic acid (PABA) on Porphyromonas gingivalis / 华西口腔医学杂志

<p><b>OBJECTIVE</b>In order to investigate the effect of PABA produced by Streptococcus sanguis on microecological balance of subgingival plaque, different concentrations of PABA were applied to see if it can influence the form and adherence of P. gingivalis.</p><p><b>METHODS</b>After adding different concentrations of PABA into 1/2 concentration of BHI media, an anaerobic technique was used to culture P. gingivalis. P. gingivalis grew in the medium was observed by a scanning electron microscope.</p><p><b>RESULTS</b>Excessively high or low PABA concentration could influence the form and adherence of P. gingivalis.</p><p><b>CONCLUSION</b>PABA produced by Streptococcus sanguis can affect the form and adherence of P. gingivalis. It indicates that Streptococcus sanguis plays regulative effect on the microecological balance of subgingival plaques.</p>

The effect of para-aminobenzoic acid on growth and metabolism of Porphyromonas gingivalis / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To study the effect of para-aminobenzoic acid (PABA) on the growth and metabolism of Porphyromonas gingivalis (P.g).</p><p><b>METHODS</b>After adding different concentrations of PABA into the medium, anaerobic technique was applied to culture P.g. The products' A value and action of TLP was assayed, and P.g grew in the medium was observed by a scanning electron microscope.</p><p><b>RESULTS</b>PABA promoted the growth of P.g and action of TLP, which would reach the highest level when PABA was 1 mg/L, and would decrease with the increasing of concentration of PABA. When the concentration arrived at 100 mg/L, PABA had no effect on them. In the mean time, PABA had effect on the form and adherence of P.g. When the concentration was 1 mg/L and 100 mg/L, this effect was strong, but as the concentration was 10 mg/L, the effect disappeared.</p><p><b>CONCLUSIONS</b>PABA influences the growth and metabolism of P.g, which indicate that Streptococcus sanguis has regulative effect on the microecology of subgingival plaque.</p>

Quantitative changes of microflora on stress-bearing areas relating to bases of removable partial dentures / 华西口腔医学杂志

<p><b>OBJECTIVE</b>This study was designed to find out the changes in microflora on the stress-bearing areas after insertion of removable partial dentures, and in turn to explore the difference between such changes caused by metal bases and acrylic resin bases respectively.</p><p><b>METHODS</b>Sixteen patients who were ready to accept removable partial dentures were selected and divided into two groups. Eight patients used metal bases while the other eight patients used acrylic resin bases. Then at the following three points: before dentures were inserted, one month after wearing and three months after wearing, the total cultivable flora were counted and the proportions of bacteria detected on the stress-bearing areas were calculated.</p><p><b>RESULTS</b>No matter what type of bases was selected, the wearing of removable partial denture enhanced the accumulation of microflora on stress-bearing areas. The percentages of Candida albicans and Lactobacilli detected on stress-bearing areas also increased significantly (P < 0.05). When such changes were compared, the authors found that the increment of Candida albicans caused by acrylic resin bases was higher than that caused by metal bases (P < 0.05).</p><p><b>CONCLUSION</b>The wearing of removable partial dentures forms new retention areas between the bases and the stress-bearing areas, as a result enhances the deposit of microbe and facilitate the growth of Candida albicans and Lactobacilli. Compared with increment of Candida albicans caused by metal bases, the increment relating to acrylic resin bases is more evident.</p>

Oral microflora of 42 patients with oral squamous cell carcinoma / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The purpose of this study was to evaluate the effects of squamous cell carcinoma on oral bacteria.</p><p><b>METHODS</b>This study investigated the microbial contents of the plaque on the surfaces of oral squamous cell carcinomas. Samples were obtained from the central surface of lesions, contiguous healthy mucosa and saliva of 42 patients with oral squamous carcinoma before and after operation.</p><p><b>RESULTS</b>The median of bacterial colony forming units per milliliter (CFUs/ml) of saliva before operation was 8.10 x 10(8) CFUs/ml. The median of CFUs per square centimeter of the tumor surface was 5.21 x 10(5) CFUs/cm2 which was significantly higher than that of the healthy (the control) mucosa (1.77 x 10(4) CFUs/cm2, P = 0.0001). The CFUs per square centimeter of mucosa surface at the operative zone was 4.34 x 10(5) CFUs/cm2 which was also significantly higher than that of the healthy (control) mucosa(7.24 x 10(4) CFUs/cm2, P = 0.0001).</p><p><b>CONCLUSION</b>Oral carcinoma can break the balance of oral microflora, which may be one of the reasons leading to the high susceptivity of these compromised patients to systemic infection.</p>

Antibacterial activities of some commonly used antibiotics to 106 isolated strains of porphyromonas ginginalis in vitro / 重庆医学

Objective Porphyromonas gingivalis(Pg)is one of the main putative pathogens of periodontal disease,espcially of adult periodontitis. This in vitro study was aimed at observing the inhibition actions of 3 kinds of antimicrobial drugs on Pg. Methods 106 stains of Pg were isolated from subgingival plaques with adult periodontitis. The agar diffusion test was used to detect the inhibition actions of metronidazole,clindamycin and tinidazole on 106 stains of Pg. Results Their MIC was respectively:0. 25mg/L,0. 6rng/L. The MIC90 of tinidazole＜MIC90 of clindamycin. The results showed:all of metroidazole, tinidazole and clindamycin can be used effectively against Pg,clindarnycin and tinidazole showed more potent antibacterial activity than metronidazole. The different concentration of Pg-liquid (l05 CFU/ml, 106 CFU/ml and 108 CFU/ml) had not influenced the results. Couclusion This study is significant to antibiotical therapy of periodontal diseases.

The effect of ceramometal bridge on the local oral flora / 实用口腔医学杂志

Objective:To investigate the effect of the ceramometal b ridge on the local gingival groove flora.Methods: Classi cal bacterial incubation and identification were used to study the changes of th e local gingival groove flora in 6 patients wearing ceramometal bridge for 1 wee k to 3 months. Results: 3 months after the prosthetic procedure the CFU o f P.melaninogenica, Gram-positive bacilli cmainly Actinomyces and the t otal bacteria were significantly increased (P