Research progress of precise therapy for Helicobacter pylori infection / 中华临床感染病杂志

Helicobacter pylori (HP) infection is one of the most prevalent chronic bacterial infections in the world, which is closely related to the development of gastrointestinal diseases, such as chronic gastritis, gastroduodenal ulcer and gastric cancer. Empirical treatment of HP infection may lead to antibiotic resistance, adverse reactions and poor compliance. The decreased HP eradication rate is related to antibiotic resistance, HP converting to coccoid form and admission of proton pump inhibitors (PPI). The implementation of precise therapy can effectively enhance the HP eradication rate, through antibiotics selecting based on detection of drug sensitivity phenotype and drug resistance genes, reducing adverse drug reactions, increasing patient compliance, and rationally administrating PPI, etc. This article reviews the research progress of precision therapy for HP infection to provide reference for clinicians.

Advances in new microfluidic chips for enrichment and detection of circulating tumor cells / 中华检验医学杂志

Due to the characteristics such as high capture, high recovery and precise control with fluid, the microfluidic chip has attracted much attention in the research field of circulating tumor cells (CTCs). The developed microfluidic system mainly included three types based on the captured principles such as biological affinity tag microfluidic chip, free label microfluidic chip and rely on biological affinity with the physical properties of integrated microfluidic chip.

Evaluation of the consistency and detection capability of seven domestic 2019-nCoV nucleic acid detection kits / 中华检验医学杂志

Objective:To compare the consistency and detection capability of seven 2019-nCoV nucleic acid detection kits, and provide reference for detection method selection of clinical laboratory and diagnosis of new coronavirus pneumonia.Methods:Two batches of pharyngeal swab samples were collected from tenpatients with confirmed infection of 2019-nCoV and 10 suspected patients with negative 2019-nCoV test results during January 29 to February 5, 2020 in Shenzhen Luohu People′s Hospital. Seven kinds of kits were labeled as ato g and used for nucleic acid detection respectively to evaluate the consistency of the test results of the clinical samples. A 2019-nCoV positive specimen was selected and diluted to 5-concentration gradient plates (Level-1 to 5) with RNase-free water. The positive detection rate and intra-batch repeatability of different brands of kits were compared.Results:The negative and positive coincidence rates of twenty clinical samples tested by six kinds of kits were 100%, and the positive and negative coincidence rate was 8/10 and 10/10 for the other kit, respectively. The results of intra-batch repeatability showed the CVs of viral loads tested by these seven kits were all less than 5%. In the concentration range of Level-1 to 3, the detection capability for open reading frame (ORF)1ab gene of Kit b,d and f was lower than Kit a,c,e and g, and the detection capability of kit e and g was the highest (14/15). The detection capability for N gene of Kit a (15/15) was higher than the other 5 kits. The comprehensive analysis of the detection capability for ORF1ab and N gene showedthat Kit d had the lowest detection capability (ORF1ab:40%,N:53%), and there was no significant difference in the detection capability of Kit a, b, c, e, and f.Conclusions:There was no significant difference in the accuracy and repeatability of the seven kits for positive samples with high viral loads, and the detection performance was good; but some kits had poor detection capability for weak positive samples. It is suggested that the weak positive samples should be rechecked by at least two manufacturers′ kits to ensure the accuracy of the results.

Advances in toxicological study and rapid detection of common mycotoxins in medicinal herbs / 中国药理学与毒理学杂志

Mycotoxins,secondary metabolites produced by certain fungi,have become one of the most harmful factors that affect the clinical safety of medicinal herbs that probably can be contaminated by harmful toxins generated from fungi in the whole process from planting to clinical use. Therefore,more toxicological research of mycotoxins,a better knowledge of the pathogenesis and quick detection with sensitivity and accuracy will play an important role in targeted therapy of poisoning by mycotoxins and early warning . In this paper,the current status of mycotoxin contamination in medicinal herbs was ana?lyzed,and the progress of toxicological study on common contaminants was reviewed. In view of the high toxicity of toxins,the strategy of ″Prevention First″ is highly desirable. Hence,the development of rapid detection of typical mycotoxins was systematically discussed. The review was intended to provide ref?erence for ensuring clinical safe administration of medicinal herbs and for reducing the risk of mycotoxin poisoning.