Determination of isofraxidin and rosmarinic acid in Zhongjiefeng Tablet by HPLC / 中成药

AIM:To determine the contents of isofraxidin and rosmarinic acid in Zhongjiefeng Tablet(Sarcandra glabra(Thunb)). METHODS:HPLC was performed on Diamonsil C18 column(250 mm ? 4. 6 mm,5 ?m),the mobile phase consisted of acetonitrile-0. 1% phosphoric acid solution(80 ∶ 20) at a flow rate of 1. 0 mL/min. The column temperature was set at 35 ℃,UV detection wavelength was at 342 nm. RESULTS:Contents of isofraxidin and rosmarinic acid detecded showed good linear relation(R2 =0. 999 99,0. 999 92,respectively) and the average recoveries(n =6) of 98. 7% (RSD =2. 22% )and 96. 9% (RSD =2. 76% ). CONCLUSION:The method is fea-sible,accurate and reliable,thereby available for quality control.