The effects of electromagnetic pulse on fluidity and lipid peroxidation of mitochondrial membrane / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study the effects of intense electromagnetic pulse(EMP) on the biological effects of mitochondrial membrane.</p><p><b>METHOD</b>Rat liver mitochondrial suspension was exposed to EMP at 60 kV/m level. The changes of membrane lipid fluidity and membrane protein mobility were detected by ESR and spin label technique. Malondialdehyde(MDA) was detected by spectrophotometer.</p><p><b>RESULTS</b>The mobility of membrane protein decreased significantly(P < 0.05). Correlation time (tau c) of control group was (0.501 +/- 0.077) x 10(-9)s, and tau c of EMP group was (0.594 +/- 0.049) x 10(-9)s, indicating that the mobility of protein was restricted. The fluidity of mitochondrial membrane increased significantly(P < 0.05) at the same time. Order parameter(S) of mitochondrial membrane lipid in control group was 0.63 +/- 0.01, while S of EMP group was 0.61 +/- 0.01(P < 0.05). MDA decreased significantly.</p><p><b>CONCLUSION</b>The mobility and lipid peroxidation of mitochondrial membrane may be disturbed after EMP exposure.</p>

STUDY ON APOPTOSIS INDUCED BY ELECTROMAGNETIC PULSE IN NEURONS OF CEREBELLAR GRANULAR LAYER / 解放军医学杂志

By observing apoptosis and morphological changes in primary culture of neurons from cerebellar granular layer, the neurons were irradiated with EMP (electromagnetic pulses), the possible injury mechanism was explored. The changes in death and apoptosis of the neurons were detected by MTT and flow cytometry, respectively. The slides stained with HE and TUNNEL were examined with light and fluorescence microscopy. The results showed that not only death of the neurons occurred immediately, but also apoptosis appeared after irradiation with EMP. Our conclusion is EMP can promote necrosis and apoptosis of neurons from cerebellar granular layer at early stage, which may result from DNA injury induced by EMP.

INFLUENCE OF EMP IRRADIATION ON LDH, AST, CHE, K+ AND Na+ EN SUPERNATANT OF CULTURED RAT PI- TUITARY CELLS / 解放军医学杂志

By observing the changes in LDH、AST、CHE、K、Na+ contents in supernatant of cultured rat pituitary cells after electromagnetic pulse (EMP) irradiation the injury mechanism to pituitary cells was explored. Pituitary cells of Wistar rat was cultured in 6-hole plates. The cells in five plates were irradiated with high field strength EMP ( electromagnetic pulse) for 5 times within 2 minutes. The electromagnetic pulse simulator provided a high electric field intensity of 60 KV/m, with rise time 20-nsec and pulse wide 30/?S. Contents of LDH, AST, CHE, K+ and Na+ in the supernatant were measured using reagent boxes (Beijing Zhongsheng High-tech Bioengineering Company) at Oh, 1h, 6h, 12h and 24h following irradiation, respeetively. All data were analyzed by statistical software Spss 8. 0. The results showed that LDH and CHE contents in the supernatant increased significantly at Oh after irradiation with EMP (P 0. 05). Our conclusion is EMP could injure membranes of pituitary cells.