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Objective: To explore influence of ginsenoside Rg1 on coronary artery angiogenesis in rats with acute myocardial infarction (AMI) and its mechanism.Methods: A total of 120 Wistar rats were randomly and equally divided into sham operation group (only received thoracotomy to expose heart without coronary ligation),AMI group (no treatment after model development with ligating left coronary artery) and ginsenoside group (received ginsenoside Rg1 injection on 3h after AMI model development).Infarct area, expressions of vascular endothelial growth factor (VEGF) and its receptor (Flk-1), and VIII factor expression were respectively measured on 24h, one week and five weeks after model development.Results: Compared with sham operation group after five weeks, there were significant rise in myocardial infarction area, number of new blood capillaries and expression levels of VEGF and its receptor Flk-1 in ginsenoside group and AMI group, P<0.05 or <0.01;compared with AMI group, there was significant reduction in myocardial infarction area [(51.31±9.67)% vs.(29.33±6.70)%], and significant rise in number of new blood capillaries [(18.31±5.07) vs.(46.79±13.67)], expressions of VEGF [greyscale value: (84.3±8.7) vs.(32.9±16.7), greyscale value was inversely proportional to expression] and Flk-1 [(17.6±8.7) vs.(59.9±16.2)] in ginsenoside group, P<0.05 or <0.01.Conclusion: Application of ginsenoside Rg1 in AMI rat model can mobilize marrow stem cells gather in ischemic myocardium, upregulate expressions of VEGF and its receptor Flk-1, effectively promote angiogenesis of blood capillaries, and reduce myocardial infarction area.
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Objective: To explore correlation among clinic blood pressure (CBP), ambulatory blood pressure (ABP) and cardiovascular diseases in diabetic populations.Methods: A total of 336 patients complicated with type 2 diabetes mellitus, who received 24h ambulatory blood pressure monitoring, were selected.According to complicated with coronary heart disease or stroke or not, they were divided into cardiovascular disease group (CVD group, n=122) and no cardiovascular disease group (NCVD group, n=214).Blood lipids, blood pressure, CBP and ABP etc.were compared between two groups;according to median of 24h mean SBP (122mmHg), they were divided into <122mmHg group (n=168) and ≥122mmHg group (n=168), incidence of cardiovascular diseases was compared between these two groups.Results: (1) Compared with NCVD group, there were significant rise in age, percentages of smoking and hypertension, and plasma hsCRP level in CVD group (P<0.05 or <0.01);for ambulatory blood pressure,there were significant rise in levels of 24h mean SBP(mSBP) [(119.8±8.7)mmHg vs.(124.4±9.6) mmHg], daytime SBP (dSBP)[(121.4±9.3) mmHg vs.(128.0±10.3) mmHg] and nighttime SBP(nSBP) [(114.4±4.2) mmHg vs.(120.8±4.7) mmHg] in CVD group, P<0.01 all;there was no significant difference in CBP between two groups;(2) compared with <122mmHg group, there were significant rise in percentages of stroke (20.2% vs.25.0%) and total cardiovascular diseases (32.7% vs.39.9%) in ≥122mmHg group, P<0.01 both;(3) Logistic regression analysis indicated that diabetic patients no matter complicated with hypertension or not, 24h mean SBP was always an independent risk factors of diabetic patients complicated cardiovascular diseases (OR=1.83, 1.36, P<0.05 all).Conclusion: ABP is superior to CBP in predicting cardiovascular risk in patients with diabetes, and 24h mean SBP may be a good ABP index to predict cardiovascular risk.
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Objective:To observe and analyze therapeutic effect of noninvasive positive pressure ventilation (NPPV) on aged patients with acute left heart failure in our hospital . Methods:A total of 128 aged patients with acute left heart failure were randomly divided into routine treatment group (n=65 ,received routine emergency drug therapy ) and NPPV group (n=63 ,received NPPV treatment based on routine treatment ) .Clinical symptoms and signs were observed and recorded in two groups ,including partial pressure of oxygen in artery (PaO2 ) ,blood pressure (BP) ,respiratory rate (RR) and heart rate (HR) .According to ECG results and combined with clinical symptoms and signs ,therapeutic effects were comprehen‐sively evaluated and compared between two groups . Results:Compared with routine treatment group ,there was significant rise in PaO2 [ (92.5 ± 7.3) mmHg vs . (106.1 ± 4.8) mmHg] ,significant reductions in BP [systolic blood pressure/dias‐tolic blood pressure ,(142.1 ± 9.2) / (97.2 ± 4.2) mmHg vs . (128.2 ± 4.1) / (89.2 ± 8.1) mmHg] ,, RR [ (27.3 ± 2.8) times/min vs . (16.3 ± 3.3) times/min] and HR [ (113.3 ± 9.3) beats/min vs . (94.2 ± 8.6) beats/min] in NPPV group ,P<0.05 all .Total effective rate of NPPV group was significantly higher than that of routine treatment group (98 . 4% vs .75 . 4% ) , P=0. 009 . Conclusion:Noninvasive positive pressure ventilation can rapidly raise PaO 2 ,relieve symp‐tom in patients with acute left heart failure ,and it is worth extending .
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Objective To explore the effects and mechanism of hydrogen sulfide on myocardial ischemia reperfusion in rats. Methods With sodium hydrogen sulfide (NaHS) as a donor of hydrogen sulfide ( H2S), we established myocardial ischemia-reperfusion injury model in rats. The SD rats were randomly divided into control group,myocardial ischemia reperfusion group (I/R group), H2S group,and H2S and glibenclamide (H2S + GLI)group. We monitored the hemodynamics index of rats, including heart rate, arterial pressure, left ventricular pressure. The rate of ventrical arrhythmia was also observed in each group. Results H2 S significantly reduced the ventricular arrhythmia (VA) occurrence (H2S group 66.5% vs I/R group 33.5% (P ＜0.05) and score in myocardial ischemia reperfusion rats (H2S group 2. 6 ±0. 7 vs I/R group 4. 5 ±0. 8(P＜0.05). The KATP channel blocker,glibenclamide,could weaken the antiarrhythmic effects of H2S ( H2S group 2. 6 ±0. 7 vs. H2S + GLI group 4. 0 ± 0. 6, P ＜ 0.05 ). Conclusions H2S has the protective effect against myocardial ischemia reperfusion damage. This function may be associated with the KATP signal transduction pathway in cells.
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Objective To explore factors affecting cardiopulmonary and cerebral resuscitation in patients with cardiac arrest. Methods Logistic regression analysis was applied to analyze the factors affecting the survival rate of patients with cardiac arrest after cardiopulmonary resuscitation and return of spontaneous circulation (ROSC). Results 6 factors were related to the patient prognosis, including where the cardiac arrest took place, when the cardiopulmonary resuscitation (CPR) began, intervals of ROSC, pupils size, PRM (postresuscitation MODS) and age. multivariant analysis showed that PRM was the primary factor affecting the patients prognosis. Conclusion The time of taking CPR was the crucial factor affecting CPR success. PRM was an independent factor affecting the survival rate of the patients after ROSC. Rapid and effective resuscitation in the first place is the key to basic life support. More emphasis should be placed on the evaluation, monitoring and protection of the function of important organs after successful CPR.