RESUMO
A method was developed for the simultaneous determination of 17 characteristic ingredients of plant extracts, including paeoniflorin, hydroxysafflor yellow A, calycosin_7_glucoside ferulic acid, etc. , in hair growth cosmetics using ultra high performance liquid chromatography ( UPLC ) . Different cosmetic samples were extracted by ultrasonic_assisted extraction with the solvent of methanol/water (4∶1, V/V) solution. After demulsified by the addition of appropriate amount of NaCl and high speed centrifugation, the supernatant was transferred and analyzed with UPLC. The separation was conducted on a Waters reversed phase column of ACQUITY UPLC CSH C18(50 mm×2. 1 mm, 1. 7μm), and the mobile phases were methanol and the solution of 0. 05% phosphate in water. The detection was performed with a photodiode_array ( PDA) detector. The linear range was 0 . 2-25 mg/L with correlation coefficients higher than 0 . 999 . The limits of detection were within 0. 3-1. 5 mg/kg, and the limits of quantification were from 1. 0 to 4. 0 mg/kg. The average recoveries of 17 characteristic ingredients were within 93 . 5%-105 . 0%, with the intra_and inter_day precision ( n=6 ) less than 4. 6%. This method was simple, rapid, with good_repeatability, and had been applied to the analysis of real samples.
RESUMO
Objective To study the chemical constituents of Ajuga decumbens. Methods Five compounds were isolated from methanol extraction and purified with silica gel column chromatography. Their structures were elucidated on the basis of spectral analysis. Results Five compounds were 1-octen-O-?-L-arabinopyranosyl-(1-6)-O-[?-D-glucopyranosyl-(1-2)]-?-D-glucopyranoside (Ⅰ), n-butyl-?-D-fructopyranoside (Ⅱ), 6, 7-dihydroxy-coumarin (Ⅲ), 5, 7-dihydroxy-4′-methylflavone (Ⅳ), and 3-O-?-D-glucopyranositosterol (Ⅴ). Conclusion 〖WT5BZ〗These five compounds are isolated from this plant for the first time.