RESUMO
In spite of no homology in sequences‚ Vip3A and Cry1Ia toxins of Bacillus thuringiensis (Bt) share common characteristics‚ such as translocation across cell membranes after synthesis at the early stage of sporulation. The aim of the present study was to compare the regulation patterns and activities of the promoters of vip3A (P
RESUMO
Objective To investigate the association between head fat and metabolic parameters in Chinese obese patients. Methods The data of 99 obese patients were collected from the outpatient department, the Tenth People's Hospital of Shanghai from September, 2011 to December, 2014. The accumulated regional fat was measured by dual-energy X-ray absorptiometry (DEXA). The body weight, body mass index (BMI), neck circumference (NC), waist circumference (WC), hip circumference (HC), visceral index, basal metabolism (BM), liver function, fasting plasma glucose (FPG), fasting insulin, fasting C peptide, blood lipid, uric acid (UA) and C-reactive protein were also obtained for analysis. Results (1) The participants consisted of 35 men and 64 women, with a mean head fat of (1.67±0.34) kg. (2) The head fat was positively correlated with body weight, BMI, NC, WC, HC, visceral index, BM, head fat percentage, arm fat, arms and legs fat, trunk fat, total fat and total muscle in both male and female participants (male: r=0.620, 0.616, 0.670, 0.678, 0.663, 0.635, 0.518, 0.635, 0.488, 0.458, 0.588, 0.618, and 0.579; female: r=0.624, 0.593, 0.610, 0.672, 0.482, 0.657, 0.647, 0.616, 0.639, 0.336, 0.467, 0.544, and 0.762, repectively; all P<0.05). In the female participants the head fat was positively correlated with fasting insulin and UA (r=0.348, 0.349,all P<0.05). For all the participants, the head fat was positively correlated with body weight, BMI, NC, WC, HC, visceral index, BM, UA, head fat percentage, upper arm fat, arms and legs fat, trunk fat, total fat, total muscle, and trunk/peripheral fat (r=0.664, 0.598, 0.685, 0.716, 0.575, 0.703, 0.610, 0.288, 0.526, 0.542, 0.346, 0.527, 0.564, 0.688, and 0.207,respectively; all P<0.05). (3) NC and WC were predictors of head fat for all subjects (β=12.802 and 29.138, both P<0.05); WC was a predictor of head fat for males (β=21.108, P<0.001); and total muscle, upper arm fat, and BM were predictors of head fat for females (β=0.046, 0.116, and-0.914, all P<0.05). Conclusion Head fat accumulation is closely related to the content and distribution of total fat and UA. Head fat may be used as an indicator for metabolic disorder risk for Chinese obese patients.
RESUMO
Objective To evaluate the effects of meal replacement (Glucerna SR, Abbort) on weight loss and fat redistribution in patients with simple obesity. Methods Twenty-two simple obesity patients received dinner replacement with Glucerna for five weeks (6 spoons, about 52 g per time, 220.48 kcal [1 772 kJ]), once a day at night replacing dinner. The weight, blood pressure, BMI, lipid, total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), fasting plasma glucose, and insulin were all observed before and after dinner replacement. The fat distribution was detected by DEXA (dual energy X-ray absorptiometry). The study was done by before-after design in the same patients. Results The body weight (P<0.001) and BMI (P<0.001) were significantly decreased after dinner replacement in our group. Dinner replacement also led to significant decrease of total fat mass (P=0.035), fat mass percentage (P=0.033) and trunk fat mass (P=0.002), while the decreases of peripheral fat mass and arm fat/leg fat mass were not significant. Lipid profiles showed that TG was significantly decreased (P=0.028)and HDL was significantly increased (P<0.001) after dinner replacement. Dinner replacement with Glucerna also resulted in a reduced HOMA-IR, though without statistical significance (P=0.372). Conclusion Dinner replacement can greatly reduce the weight of obese patients, improving fat distribution and regulating blood lipid and glucose. Dinner replacement is tolerable and safe, and it can improve the metabolism of obese patients.
RESUMO
<p><b>BACKGROUND AND OBJECTIVE</b>Primary renal synovial sarcoma is rare and might be misdiagnosed as another renal tumor. This study demonstrates the clinicopathologic and immunohistochemical features, differential diagnosis, and prognosis of such tumors.</p><p><b>METHODS</b>Histologic slides and clinical data were reviewed for 4 patients with primary renal synovial sarcoma and immunohistochemical staining was performed. Molecular analysis was performed on 2 cases to demonstrate the presence of the SYT-SSX gene fusion transcripts by reverse transcriptase polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The patients were 2 women and 2 men aged from 32 to 48 years. The tumors were 10.0-15.0 cm in diameter, grey-white and solid, and hemorrhage or necrosis was observed. Microscopically, the tumors consisted of mitotically active, monomorphic plump spindle cells with indistinct cell borders growing in short, intersecting fascicles. Hypocellular myxoid areas and a prominent hemangiopericytomatous pattern were present in all cases. The average mitotic rate was 5-8 mitoses/10 high-power fields. Hemorrhage and tumor necrosis were easily found. Scattered small cysts lined with flat, cuboidal, or hobnailed epithelia were found in 3 cases. Tumor cells are immunoreactive for Vimentin (4/4), Bcl-2 (4/4), CD99 (4/4), and CD56 (3/4), and focally for EMA (3/4) and Cytokeratin (3/4). SYT-SSX1 gene fusion was detected in the 2 cases in which RT-PCR analysis was performed. One patient had tumor metastasis to the lung 6 months after surgery and died 5 months later. Multiple metastasis to the liver occurred in one patient and the patient died 13 months after the initial surgery. The other 2 patients had tumors recur at 8 and 15 months and died at 18 and 21 months, respectively, after the initial operation.</p><p><b>CONCLUSION</b>Primary renal synovial sarcoma is rare, with poor prognosis, characterized by SYT-SSX gene fusion, and needs to be differentiated from other renal sarcomas.</p>