Influence of sodium hypochlorite irrigants on the bonding of fiber posts to root canals: a Meta-analysis / 中国组织工程研究

BACKGROUND: Currently, whether the use of sodium hypochlorite (NaOCl) influences the bond strength of fiber posts is still uncertain. OBJECTIVE: To evaluate the influence of NaOCl on the bond strength of fiber posts by Meta-analysis. METHODS: Randomized controlled trials about the impact of NaOCl irrigation to the bond strength of fiber posts were retrieved from PubMed, EMbase, Web Of Science, OVID, CBM, ClinicalKey, CNKI, CqVip and WanFang databases, and other databases through manual retrieval as well. The deadline of the collection was December 31, 2016, with no limit of regions and languages. A Meta-analysis was performed with the bond strength of fiber posts as an indicator. RESULTS: A total of 11 randomized controlled trials were included. In the experimental group (NaOCl group), the root canal was rinsed with NaOCl, and dried followed by fiber post bonding using the total-etching system, the self-etching system and the self-adhesive system. In the control group, the root canal was washed with pure water or normal saline.Meta-analysis showed that weighted mean difference(WMD)(NaOCl group/control group)=-0.90,95% confidence interval(CI)(-1.50,-0.31),Z=2.97,P=0.003.Subgroup analysis showed that WMD(total-etching:NaOCl group/control group)=-2.38,95% CI(-4.86,0.10),Z=1.88,P=0.06;WMD(self-etching:NaOCl group/control group)=-1.22,95% CI(-2.17,-0.28),Z=2.55, P=0.01;WMD(self-adhesive:NaOCl group/control group)=-1.97,95% CI(-2.30,-1.64),Z=11.73,P<0.000 01.As the limited number of enrolled samples in the total-etching group and self-etching group results in a higher heterogeneity, further investigations are warranted. The present data suggests that irrigating the surface of root canals with NaOCl alone has negative impact on the bond strength of fiber posts using the self-adhesive system.

Surgical Treatment Strategies of Intravenous Leiomyomatosis withRight Cardiac Cavities Extension / 中国医学科学院学报

Objective To investigate the diagnosis and surgical treatment strategies of intravenous leiomyomatosis(IVL)extending through inferior vena cava into the right cardiac cavities. Methods Thirty patients of IVL extending through inferior vena cava into the right cardiac cavities were treated in Peking Union Medical College Hospital from November 2002 to January 2015.The following variables were studied: age,cardiopulmonary bypass time,deep hypothermic circulatory arrest time,origins of IVL,blood loss,duration of post-operative hospital stay,hospitalization expenses,edema of lower extremity,blood transfusion,postoperative complication,residual IVL,and re-grow or recurrence. Results Thirteen of 30 patients reported double lower limb edema. The cardiopulmonary bypass was applied in 27 cases,and the average duration of cardiopulmonary bypass was(106.9±53.7)min. Then,21 patients were treated with the deep hypothermic circulatory arrest,and the mean time was(28.2±11.6) min. The tumors originated from the genital veins in 9 cases,the iliac vein in 13 cases,and both veins in 8 cases. The average intra-operative blood loss volume was (2060.5±2012.3)ml,and 21 patients received blood transfusion. The average hospitalization time was(18.9±8.3)days and the average hospitalization expenses was (80 840.4±28 264.2)RMB yuan. While 14 patients had postoperative complications,there was no serious postoperative complication or death.All patients have shown a favorable outcome.Conclusions Tumor embolus extending through inferior vena cava into the right cardiac cavities should be suspected in patients with multiple hysteromyoma. Successful therapy for IVL with right cardiac cavities extension is dependent on reasonable surgical treatment strategies. Surgical removal of the ovaries is vital to avoid IVL re-grow or recurrence.

Endoscopic Saphenous Vein Harvesting versus Open Vein Harvesting Techniques / 中国医学科学院学报

<p><b>OBJECTIVE</b>To investigate whether endoscopic saphenous vein harvesting (EVH )reduces leg wound morbidity and improves rehabilitation of lower extremity as compared to open vein harvesting (OVH) in patients with coronary artery disease.</p><p><b>METHODS</b>The clinical data of 333 patients who underwent isolated coronary artery bypass graft surgery (CABG) between January 2007 and December 2011 were retrospectively analyzed.These patients were divided into EVH group (n=210) and OVH group (n=123). Parameters including age,sex,with/without diabetes mellitus,leg wound complications rate,postoperative hospital stay,hospitalization expense,and vein graft patency were analyzed.</p><p><b>RESULTS</b>The age,sex,with/without diabetes mellitus were not significantly different between two groups (P>0.05). However,the incidences of leg wound complications, poor wound healing, wound infections, wound pain,wound numbness, and edema of lower extremity were significantly lower in the EVH group than the OVH group (P<0.05). The ecchymoma and deep vein thrombosis incidences were not significantly different between these two groups (P>0.05). The postoperative hospital stay showed no significant difference (P>0.05), while the hospitalization expense was significantly higher in the EVH group than in the OVH group (P<0.05). There was no statistical difference of vein graft patency (P>0.05).</p><p><b>CONCLUSION</b>The EVH system is a safe and effective alternative to OVH because it can markedly reduce postoperative leg wound morbidity and the EVH vein graft has good patency.</p>

Analyzing the production of organic acid of different genotype streptococcus mutans isolated from children with different caries experience / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To compare the production of organic acid of different genotype Streptococcus mutans (S. mutans) isolated from children with different caries experience.</p><p><b>METHODS</b>66 strains of S.mutans isolated from dental plaques of children aged from 3 to 5 with different caries experience were chosen as test bacteria. The quantities of organic acid include formic acid, acetic acid and lactic acid which produced by different genotype of S. mutans, were measured by gas chromatograph.</p><p><b>RESULTS</b>There were significant difference in production of organic acid among the different genotypes of S. mutans isolated from children with different caries susceptibility, and so were the strains isolated from children within the same caries susceptibility (P < 0.05). The more genotypes the strain had, the more organic acid it produced (P < 0.05). Among all the organic acid, the quantity of lactic acid was much more than that of formic acid and acetic acid.</p><p><b>CONCLUSIONS</b>There were significant difference in the ability of the strains with different genotypes to produce organic acid, and the more genotypes it has, the more organic acid it produced.</p>

Influence of Environmental Temperature on Formaldehyde Emission from Blockboard / 环境与健康杂志

Objective To study the effect of temperature on the emission of tbrmaldehyde trom the blockboard.Methods A 0.25 m3 simulation chamber was used to explore the emission of formaldehyde from blockboard at the temperature of 15,17,19,21, 23,25,27,and 29 ℃,respectively.The relative humidity,the ventilation velocity and the loading factor in simulation chamber was (50?1)%,1.0 L/min,and(1?0.03)m~2/m~3 respectively.Results A hyperbolic logarithm variation in formaldehyde concentration with time in the chamber was observed at different temperatures.Formaldehyde concentration in the chamber increased remarkably once the temperature exceeded 25 C.The chamber stabilization formaldehyde concentration varied with temperature in accordance with an exponent equation of y=0.921 9?0.024 9e~(0.176 4x)(R~2=0.996 9).Conclusion The temperature can greatly influence formaldehyde emission from the blockboards.Higher temperature will facilitate formaldehyde emission from the blockboards.

Establishment and application of a RT-PCR system for quantifying mRNA of 4 hsp genes in human cells / 中国医学科学院学报

<p><b>OBJECTIVE</b>To establish a RT-PCR system for detecting mRNA expression level of 4 hsp genes in human cells.</p><p><b>METHODS</b>RT-PCR system was established with gene cloning, gene recombination, in vitro transcription and RT-PCR techniques; The detection of expression level of 4 hsp genes in SW13 cell was carried out with this system.</p><p><b>RESULTS</b>In SW13 cell, hsp70 and hsp90 alpha were typical heat shock induced genes, while hsp60 and hsp90 beta were efficiently expressed and further induced by heat-shock to various extent.</p><p><b>CONCLUSIONS</b>In our hands novel RT-PCR system can be used to detect mRNA expression level of 4 human hsp genes.</p>