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Objective To study the effect of angiotensinⅡon the expression of monocyte chemoattractant protein-1 (MCP-1) in cultured human umbilical vein endothelial cells(hUVEC) , and the effect of peroxisome proliferator- activated receptors (PPARs)?and?on MCP-1. Methods MCP-1 protein level was detected by enzyme-linked immunosorbent assay (ELISA) method, and the mRNA expression level of MCP-1 was determined by RT-PCR. Results AngiotensinⅡdistinctly increased the expression of MCP-1 in a dose-dependent manner in cultured hUVECs, and valsartan inhibited the expression of MCP-1 remarkably. Both rosiglitazone (PPAR7 agonist) and fenofibrate (PPAR?agonist) concentration- dependently reduced the expression of MCP-1 in induced by AngⅡ10-6 mol/L. Conclusions AngiotensinⅡcan increase the expression of MCP-1 evidently in hUVECs, which is inhibited by valsartan. The activation of PPARa and PPAR?can decrease the expression of MCP-1 in hUVECs.
RESUMO
Objective To investigate the clinical value of the plasma N-terminal pro-brain natriuretic peptide levels in patients with acute cardiac dyspnea or noncardiac dyspnea.Methods 93 patients with acute dyspnea were divided into two groups:cardiac dyspnea group(61 cases)and noncardiac dyspnea group(32 cases).In cardiac dyspnea group,according to the results of echocardiography,the cases were divided into three groups:pure diastolic heart failure(25 cases),pure systolic heart failure(21 cases)as well as diastolic and systolic heart failure(15 cases).At the same time,heart function was classified according to NYHA.The plasma NT-proBNP level was measured by ELISA.The data was analyzed by SPSS12.0.Results The plasma NT-proBNP level of the cases with cardiac dyspnea and noncardiac dyspnea was(3.591 ?2.943)ng/L and(0.429 ?0.119)ng/L respectively.There was a significant difference between cardiac dyspnea cases and noncardiac dyspnea cases(P